Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/Oligonucleotide_synthesis> ?p ?o }
- Oligonucleotide_synthesis abstract "Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not have this limitation, although it is, most often, carried out in the opposite, 3' to 5' direction. Currently, the process is implemented as solid-phase synthesis using phosphoramidite method and phosphoramidite building blocks derived from protected 2'-deoxynucleosides (dA, dC, dG, and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, e.g. LNA, BNA.To obtain the desired oligonucleotide, the building blocks are sequentially coupled to the growing oligonucleotide chain in the order required by the sequence of the product (see Synthetic cycle below). The process has been fully automated since the late 1970s. Upon the completion of the chain assembly, the product is released from the solid phase to solution, deprotected, and collected. The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity. Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15–25 bases in length.Oligonucleotides find a variety of applications in molecular biology and medicine. They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes.".
- Oligonucleotide_synthesis thumbnail Todd_synthesis.png?width=300.
- Oligonucleotide_synthesis wikiPageExternalLink OligoDesigner.aspx.
- Oligonucleotide_synthesis wikiPageExternalLink chitem_print.aspx?aid=2894&chid=0.
- Oligonucleotide_synthesis wikiPageID "10663351".
- Oligonucleotide_synthesis wikiPageLength "79046".
- Oligonucleotide_synthesis wikiPageOutDegree "159".
- Oligonucleotide_synthesis wikiPageRevisionID "706369790".
- Oligonucleotide_synthesis wikiPageWikiLink 1-Methylimidazole.
- Oligonucleotide_synthesis wikiPageWikiLink 2,6-Lutidine.
- Oligonucleotide_synthesis wikiPageWikiLink 4-Dimethylaminopyridine.
- Oligonucleotide_synthesis wikiPageWikiLink 4-Toluenesulfonyl_chloride.
- Oligonucleotide_synthesis wikiPageWikiLink AP_site.
- Oligonucleotide_synthesis wikiPageWikiLink Acetic_anhydride.
- Oligonucleotide_synthesis wikiPageWikiLink Acetonitrile.
- Oligonucleotide_synthesis wikiPageWikiLink Acrylonitrile.
- Oligonucleotide_synthesis wikiPageWikiLink Adenine.
- Oligonucleotide_synthesis wikiPageWikiLink Adenosine.
- Oligonucleotide_synthesis wikiPageWikiLink Alexander_R._Todd.
- Oligonucleotide_synthesis wikiPageWikiLink Ammonia.
- Oligonucleotide_synthesis wikiPageWikiLink Ammonium_hydroxide.
- Oligonucleotide_synthesis wikiPageWikiLink Artificial_gene_synthesis.
- Oligonucleotide_synthesis wikiPageWikiLink Atomic_mass.
- Oligonucleotide_synthesis wikiPageWikiLink Atomic_mass_unit.
- Oligonucleotide_synthesis wikiPageWikiLink Azole.
- Oligonucleotide_synthesis wikiPageWikiLink Base_(chemistry).
- Oligonucleotide_synthesis wikiPageWikiLink Biotin.
- Oligonucleotide_synthesis wikiPageWikiLink Bridged_nucleic_acid.
- Oligonucleotide_synthesis wikiPageWikiLink Capillary_electrophoresis–mass_spectrometry.
- Oligonucleotide_synthesis wikiPageWikiLink Carbon_tetrachloride.
- Oligonucleotide_synthesis wikiPageWikiLink Category:Laboratory_techniques.
- Oligonucleotide_synthesis wikiPageWikiLink Category:Molecular_biology.
- Oligonucleotide_synthesis wikiPageWikiLink Chirality_(chemistry).
- Oligonucleotide_synthesis wikiPageWikiLink Cholesterol.
- Oligonucleotide_synthesis wikiPageWikiLink Collidine.
- Oligonucleotide_synthesis wikiPageWikiLink Covalent_bond.
- Oligonucleotide_synthesis wikiPageWikiLink Cross-link.
- Oligonucleotide_synthesis wikiPageWikiLink Cytidine.
- Oligonucleotide_synthesis wikiPageWikiLink Cytosine.
- Oligonucleotide_synthesis wikiPageWikiLink DNA.
- Oligonucleotide_synthesis wikiPageWikiLink DNA_sequencing.
- Oligonucleotide_synthesis wikiPageWikiLink Deconvolution.
- Oligonucleotide_synthesis wikiPageWikiLink Deoxyadenosine.
- Oligonucleotide_synthesis wikiPageWikiLink Deoxycytidine.
- Oligonucleotide_synthesis wikiPageWikiLink Deoxyguanosine.
- Oligonucleotide_synthesis wikiPageWikiLink Depurination.
- Oligonucleotide_synthesis wikiPageWikiLink Diastereomer.
- Oligonucleotide_synthesis wikiPageWikiLink Dichloroacetic_acid.
- Oligonucleotide_synthesis wikiPageWikiLink Dichloromethane.
- Oligonucleotide_synthesis wikiPageWikiLink Diethylamine.
- Oligonucleotide_synthesis wikiPageWikiLink Disulfiram.
- Oligonucleotide_synthesis wikiPageWikiLink Divinylbenzene.
- Oligonucleotide_synthesis wikiPageWikiLink Electrospray_ionization.
- Oligonucleotide_synthesis wikiPageWikiLink Enzyme.
- Oligonucleotide_synthesis wikiPageWikiLink Ethanol_precipitation.
- Oligonucleotide_synthesis wikiPageWikiLink Fluorescein.
- Oligonucleotide_synthesis wikiPageWikiLink Fluorescein_amidite.
- Oligonucleotide_synthesis wikiPageWikiLink Gel_electrophoresis.
- Oligonucleotide_synthesis wikiPageWikiLink Genetic_code.
- Oligonucleotide_synthesis wikiPageWikiLink Guanosine.
- Oligonucleotide_synthesis wikiPageWikiLink Har_Gobind_Khorana.
- Oligonucleotide_synthesis wikiPageWikiLink High-performance_liquid_chromatography.
- Oligonucleotide_synthesis wikiPageWikiLink Hybridization_probe.
- Oligonucleotide_synthesis wikiPageWikiLink Hydrolysis.
- Oligonucleotide_synthesis wikiPageWikiLink Imidazole.
- Oligonucleotide_synthesis wikiPageWikiLink In_vitro.
- Oligonucleotide_synthesis wikiPageWikiLink In_vivo.
- Oligonucleotide_synthesis wikiPageWikiLink Iodine.
- Oligonucleotide_synthesis wikiPageWikiLink Ion_exchange.
- Oligonucleotide_synthesis wikiPageWikiLink Liquid_chromatography–mass_spectrometry.
- Oligonucleotide_synthesis wikiPageWikiLink Litre.
- Oligonucleotide_synthesis wikiPageWikiLink Locked_nucleic_acid.
- Oligonucleotide_synthesis wikiPageWikiLink Mass_spectrum.
- Oligonucleotide_synthesis wikiPageWikiLink ionization.
- Oligonucleotide_synthesis wikiPageWikiLink Methanol.
- Oligonucleotide_synthesis wikiPageWikiLink Methylamine.
- Oligonucleotide_synthesis wikiPageWikiLink Michael_reaction.
- Oligonucleotide_synthesis wikiPageWikiLink Mole_(unit).
- Oligonucleotide_synthesis wikiPageWikiLink Molecular_mass.
- Oligonucleotide_synthesis wikiPageWikiLink Mutation.
- Oligonucleotide_synthesis wikiPageWikiLink N,N-Dicyclohexylcarbodiimide.
- Oligonucleotide_synthesis wikiPageWikiLink Nuclease.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleic_acid.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleic_acid_analogue.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleic_acid_hybridization.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleic_acid_sequence.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleoside.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleoside_phosphoramidite.
- Oligonucleotide_synthesis wikiPageWikiLink Nucleotide.
- Oligonucleotide_synthesis wikiPageWikiLink Oligonucleotide.
- Oligonucleotide_synthesis wikiPageWikiLink Oligonucleotide_synthesis.
- Oligonucleotide_synthesis wikiPageWikiLink Peptide_nucleic_acid.
- Oligonucleotide_synthesis wikiPageWikiLink Peptide_synthesis.
- Oligonucleotide_synthesis wikiPageWikiLink Phosphate.
- Oligonucleotide_synthesis wikiPageWikiLink Phosphite.
- Oligonucleotide_synthesis wikiPageWikiLink Phosphodiester_bond.
- Oligonucleotide_synthesis wikiPageWikiLink Phosphorus.
- Oligonucleotide_synthesis wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Oligonucleotide_synthesis wikiPageWikiLink Polyethylene_glycol.
- Oligonucleotide_synthesis wikiPageWikiLink Polymerase_chain_reaction.