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- Nick_translation abstract "Nick translation(or head translation), developed in 1977 by Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in Fluorescent in situ hybridization or blotting techniques. It can also be used for radiolabeling.This process is called nick translation because the DNA to be processed is treated with DNase to produce single-stranded \"nicks.\" This is followed by replacement in nicked sites by DNA polymerase I, which elongates the 3' hydroxyl terminus, removing nucleotides by 5'-3' exonuclease activity, replacing them with dNTPs. To radioactively label a DNA fragment for use as a probe in blotting procedures, one of the incorporated nucleotides provided in the reaction is radiolabeled in the alpha phosphate position. Similarly, a fluorophore can be attached instead for fluorescent labelling, or an antigen for immunodetection. When DNA polymerase I eventually detaches from the DNA, it leaves another nick in the phosphate backbone. The nick has \"translated\" some distance depending on the processivity of the polymerase. This nick could be sealed by DNA ligase, or its 3' hydroxyl group could serve as the template for further DNA polymerase I activity. Proprietary enzyme mixes are available commercially to perform all steps in the procedure in a single incubation.Nick translation could cause double-stranded DNA breaks, if DNA polymerase I encounters another nick on the opposite strand, resulting in two shorter fragments. This does not influence the performance of the labelled probe in in-situ hybridization.".
- Nick_translation wikiPageID "2173109".
- Nick_translation wikiPageLength "2621".
- Nick_translation wikiPageOutDegree "15".
- Nick_translation wikiPageRevisionID "609650070".
- Nick_translation wikiPageWikiLink Blot_(biology).
- Nick_translation wikiPageWikiLink Category:Genetics.
- Nick_translation wikiPageWikiLink Category:Laboratory_techniques.
- Nick_translation wikiPageWikiLink Category:Molecular_biology.
- Nick_translation wikiPageWikiLink DNA.
- Nick_translation wikiPageWikiLink DNA_ligase.
- Nick_translation wikiPageWikiLink DNA_polymerase_I.
- Nick_translation wikiPageWikiLink Deoxyribonucleotide.
- Nick_translation wikiPageWikiLink Directionality_(molecular_biology).
- Nick_translation wikiPageWikiLink Fluorescence_in_situ_hybridization.
- Nick_translation wikiPageWikiLink Fluorescent_tag.
- Nick_translation wikiPageWikiLink Molecular_biology.
- Nick_translation wikiPageWikiLink Processivity.
- Nick_translation wikiPageWikiLink Radioactive_tracer.
- Nick_translation wikiPageWikiLinkText "Nick Translation".
- Nick_translation wikiPageWikiLinkText "Nick translation".
- Nick_translation wikiPageWikiLinkText "nick translated".
- Nick_translation wikiPageWikiLinkText "nick translation".
- Nick_translation wikiPageUsesTemplate Template:Genetics-stub.
- Nick_translation wikiPageUsesTemplate Template:Refimprove.
- Nick_translation wikiPageUsesTemplate Template:Reflist.
- Nick_translation subject Category:Genetics.
- Nick_translation subject Category:Laboratory_techniques.
- Nick_translation subject Category:Molecular_biology.
- Nick_translation hypernym Technique.
- Nick_translation type TopicalConcept.
- Nick_translation type Laboratory.
- Nick_translation type Technique.
- Nick_translation comment "Nick translation(or head translation), developed in 1977 by Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in Fluorescent in situ hybridization or blotting techniques.".
- Nick_translation label "Nick translation".
- Nick_translation sameAs Q371037.
- Nick_translation sameAs Nick-translation.
- Nick_translation sameAs Nick_translation.
- Nick_translation sameAs Nick_translation.
- Nick_translation sameAs m.06s922.
- Nick_translation sameAs Q371037.
- Nick_translation wasDerivedFrom Nick_translation?oldid=609650070.
- Nick_translation isPrimaryTopicOf Nick_translation.