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- Lysogeny_broth abstract "Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The initialism is also commonly, albeit incorrectly, taken to mean Luria broth, Lennox broth, or Luria-Bertani medium. According to its creator Giuseppe Bertani, the abbreviation LB was actually intended to stand for lysogeny broth. The formula of the LB medium was published in 1951 in the first paper of Bertani on lysogeny. In this article he described the modified single-burst experiment and the isolation of the phages P1, P2, and P3. He had developed the LB medium to optimize Shigella growth and plaque formation.LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. It continues to be one of the most common media used for maintaining and cultivating laboratory recombinant strains of Escherichia coli. For physiological studies however, the use of LB medium is to be discouraged.There are several common formulations of LB. Although they are different, they generally share a somewhat similar composition of ingredients used to promote growth, including the following: Peptides and casein peptones Vitamins (including B vitamins) Trace elements (e.g. nitrogen, sulfur, magnesium) MineralsPeptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth.In his original 1951 paper, Bertani used 10 grams of NaCl and 1 gram of glucose per 1 L of solution; Luria in his \"L broth\" of 1957 copied Bertani's original recipe exactly. Recipes published later have typically left out the glucose.".
- Lysogeny_broth thumbnail LBmedium.JPG?width=300.
- Lysogeny_broth wikiPageID "2086023".
- Lysogeny_broth wikiPageLength "7553".
- Lysogeny_broth wikiPageOutDegree "41".
- Lysogeny_broth wikiPageRevisionID "704578606".
- Lysogeny_broth wikiPageWikiLink Agar_plate.
- Lysogeny_broth wikiPageWikiLink Amino_acid.
- Lysogeny_broth wikiPageWikiLink Autoclave.
- Lysogeny_broth wikiPageWikiLink Bacteria.
- Lysogeny_broth wikiPageWikiLink Bacterial_growth.
- Lysogeny_broth wikiPageWikiLink Bacteriophage_P2.
- Lysogeny_broth wikiPageWikiLink Broth.
- Lysogeny_broth wikiPageWikiLink Casein.
- Lysogeny_broth wikiPageWikiLink Category:Microbiological_media.
- Lysogeny_broth wikiPageWikiLink DNA.
- Lysogeny_broth wikiPageWikiLink Distilled_water.
- Lysogeny_broth wikiPageWikiLink Enterobacteria_phage_P3.
- Lysogeny_broth wikiPageWikiLink Escherichia_coli.
- Lysogeny_broth wikiPageWikiLink Escherichia_coli_(molecular_biology).
- Lysogeny_broth wikiPageWikiLink Giuseppe_Bertani.
- Lysogeny_broth wikiPageWikiLink Growth_medium.
- Lysogeny_broth wikiPageWikiLink Lysogenic_cycle.
- Lysogeny_broth wikiPageWikiLink Molecular_biology.
- Lysogeny_broth wikiPageWikiLink Molecular_microbiology.
- Lysogeny_broth wikiPageWikiLink Nutrient.
- Lysogeny_broth wikiPageWikiLink Organic_compound.
- Lysogeny_broth wikiPageWikiLink P1_phage.
- Lysogeny_broth wikiPageWikiLink Peptide.
- Lysogeny_broth wikiPageWikiLink Plasmid.
- Lysogeny_broth wikiPageWikiLink Purified_water.
- Lysogeny_broth wikiPageWikiLink Recombinant_DNA.
- Lysogeny_broth wikiPageWikiLink Salvador_Luria.
- Lysogeny_broth wikiPageWikiLink Shigella.
- Lysogeny_broth wikiPageWikiLink Single-burst_experiment.
- Lysogeny_broth wikiPageWikiLink Sodium_chloride.
- Lysogeny_broth wikiPageWikiLink Super_Optimal_Broth.
- Lysogeny_broth wikiPageWikiLink Tryptone.
- Lysogeny_broth wikiPageWikiLink Virus_quantification.
- Lysogeny_broth wikiPageWikiLink Vitamin.
- Lysogeny_broth wikiPageWikiLink Yeast_extract.
- Lysogeny_broth wikiPageWikiLink File:LBmedium.JPG.
- Lysogeny_broth wikiPageWikiLink File:Laboratoorne_sööde_%22LB%22_ehk_%22lysogeny_broth%22_on_sobiv_bakterite_kasvatamiseks..JPG.
- Lysogeny_broth wikiPageWikiLinkText "LB".
- Lysogeny_broth wikiPageWikiLinkText "Lysogeny broth".
- Lysogeny_broth wikiPageWikiLinkText "lysogeny broth".
- Lysogeny_broth wikiPageUsesTemplate Template:Reflist.
- Lysogeny_broth wikiPageUsesTemplate Template:Unreferenced_section.
- Lysogeny_broth subject Category:Microbiological_media.
- Lysogeny_broth type Redirect.
- Lysogeny_broth comment "Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The initialism is also commonly, albeit incorrectly, taken to mean Luria broth, Lennox broth, or Luria-Bertani medium. According to its creator Giuseppe Bertani, the abbreviation LB was actually intended to stand for lysogeny broth. The formula of the LB medium was published in 1951 in the first paper of Bertani on lysogeny.".
- Lysogeny_broth label "Lysogeny broth".
- Lysogeny_broth sameAs Q1797887.
- Lysogeny_broth sameAs LB-Medium.
- Lysogeny_broth sameAs Caldo_de_Lisogenia.
- Lysogeny_broth sameAs LB_(milieu_de_culture).
- Lysogeny_broth sameAs LB培地.
- Lysogeny_broth sameAs Pożywka_LB.
- Lysogeny_broth sameAs m.06l5_9.
- Lysogeny_broth sameAs Среда_LB.
- Lysogeny_broth sameAs Q1797887.
- Lysogeny_broth sameAs LB培养基.
- Lysogeny_broth wasDerivedFrom Lysogeny_broth?oldid=704578606.
- Lysogeny_broth depiction LBmedium.JPG.
- Lysogeny_broth isPrimaryTopicOf Lysogeny_broth.