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- Inverse_polymerase_chain_reaction abstract "Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is available from which primers may be designed.Inverse PCR is especially useful for the determination of insert locations. For example, various retroviruses and transposons randomly integrate into genomic DNA. To identify the sites where they have entered, the known, \"internal\" viral or transposon sequences can be used to design primers that will amplify a small portion of the flanking, \"external\" genomic DNA. The amplified product can then be sequenced and compared with DNA databases to locate the sequence which has been disrupted.The inverse PCR method involves a series of restriction digests and ligation, resulting in a looped fragment that can be primed for PCR from a single section of known sequence. Then, like other polymerase chain reaction processes, the DNA is amplified by the temperature-sensitive DNA polymerase: A target region with an internal section of known sequence and unknown flanking regions is identified Genomic DNA is digested into fragments of a few kilobases by a usually low-moderate frequency (6-8 base) cutting restriction enzyme. Under low DNA concentrations, self-ligation is induced to give a circular DNA product. PCR is carried out as usual, with primers complementary to sections of the known internal sequence.*Finally the sequence is compared with the sequence available in the data base.Note: although the figure suggests that the circularized ligation product is digested prior to PCR, this is not the case. PCR does not require linear products and the use of another restriction enzyme to cut the known sequence could also cut within the unknown region, resulting in a failed PCR.".
- Inverse_polymerase_chain_reaction thumbnail Inverse_PCR.png?width=300.
- Inverse_polymerase_chain_reaction wikiPageID "3567878".
- Inverse_polymerase_chain_reaction wikiPageLength "2662".
- Inverse_polymerase_chain_reaction wikiPageOutDegree "18".
- Inverse_polymerase_chain_reaction wikiPageRevisionID "568254929".
- Inverse_polymerase_chain_reaction wikiPageWikiLink BLAST.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Base_pair.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Category:Laboratory_techniques.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Category:Molecular_biology.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Category:Polymerase_chain_reaction.
- Inverse_polymerase_chain_reaction wikiPageWikiLink DNA.
- Inverse_polymerase_chain_reaction wikiPageWikiLink DNA_ligase.
- Inverse_polymerase_chain_reaction wikiPageWikiLink DNA_polymerase.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Directionality_(molecular_biology).
- Inverse_polymerase_chain_reaction wikiPageWikiLink File:Inverse_PCR.png.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Genomic_DNA.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Insert_(molecular_biology).
- Inverse_polymerase_chain_reaction wikiPageWikiLink Polymerase_chain_reaction.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Primer_(molecular_biology).
- Inverse_polymerase_chain_reaction wikiPageWikiLink Restriction_digest.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Restriction_enzyme.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Retrovirus.
- Inverse_polymerase_chain_reaction wikiPageWikiLink Transposable_element.
- Inverse_polymerase_chain_reaction wikiPageWikiLinkText "Inverse PCR".
- Inverse_polymerase_chain_reaction wikiPageWikiLinkText "Inverse polymerase chain reaction".
- Inverse_polymerase_chain_reaction wikiPageUsesTemplate Template:Cite_web.
- Inverse_polymerase_chain_reaction wikiPageUsesTemplate Template:No_footnotes.
- Inverse_polymerase_chain_reaction wikiPageUsesTemplate Template:PCR.
- Inverse_polymerase_chain_reaction wikiPageUsesTemplate Template:Portal_bar.
- Inverse_polymerase_chain_reaction subject Category:Laboratory_techniques.
- Inverse_polymerase_chain_reaction subject Category:Molecular_biology.
- Inverse_polymerase_chain_reaction subject Category:Polymerase_chain_reaction.
- Inverse_polymerase_chain_reaction hypernym Variant.
- Inverse_polymerase_chain_reaction type Laboratory.
- Inverse_polymerase_chain_reaction type Method.
- Inverse_polymerase_chain_reaction type Technique.
- Inverse_polymerase_chain_reaction comment "Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is available from which primers may be designed.Inverse PCR is especially useful for the determination of insert locations.".
- Inverse_polymerase_chain_reaction label "Inverse polymerase chain reaction".
- Inverse_polymerase_chain_reaction sameAs Q475229.
- Inverse_polymerase_chain_reaction sameAs Inverse_Polymerase-Kettenreaktion.
- Inverse_polymerase_chain_reaction sameAs IPCR.
- Inverse_polymerase_chain_reaction sameAs m.09m1vc.
- Inverse_polymerase_chain_reaction sameAs معکوس_پیسیار.
- Inverse_polymerase_chain_reaction sameAs Q475229.
- Inverse_polymerase_chain_reaction wasDerivedFrom Inverse_polymerase_chain_reaction?oldid=568254929.
- Inverse_polymerase_chain_reaction depiction Inverse_PCR.png.
- Inverse_polymerase_chain_reaction isPrimaryTopicOf Inverse_polymerase_chain_reaction.