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- Interferometric_scattering_microscopy abstract "Interferometric scattering microscopy (iSCAT) is an optical microscopy technique that relies on collecting light scattered by an object together with a reference light field, provided by the reflection at an interface. In the presence of the scattering object, the total intensity of light returning in the direction of the incident beam is different than in the absence of that object due to interference of the scattered and reflected light. Thus, the object appears as a small dip on top of a large background.An iSCAT microscope requires illumination of the sample and collection of reflected and scattered light, which interfere at the detector. The concept is different compared to dark field microscopy, in the sense that iSCAT doesn't reject the reflected (background) light. The signal has therefore three main components: (1) reflected light, which dominates the iSCAT image and which is reduced to a minimum in dark field microscopy; (2) scattered light, which is negligible in iSCAT but the main signal in a dark field image; (3) interference term, which modulates the signal originating from reflection proportional to the scattering amplitude. iSCAT signal scales linearly with the scattering amplitude and thus particle's volume (it is V^2 in case of dark field scattering detection). The basic design of an interferometric detection has been explored for decades in interference reflection microscopy and differential interference contrast microscopy. The major difference between those techniques and iSCAT is that the latter uses a coherent light source, which increases the interferometric contrast.Biological applications: label-free virus tracking GM1 tracking molecular motors tracking label-free imaging of lipid vesicles, lipid nanodomains, or myosin 5".
- Interferometric_scattering_microscopy thumbnail Principle_of_iSCAT_detection_and_basic_iSCAT_setup.png?width=300.
- Interferometric_scattering_microscopy wikiPageID "48622157".
- Interferometric_scattering_microscopy wikiPageLength "3906".
- Interferometric_scattering_microscopy wikiPageOutDegree "9".
- Interferometric_scattering_microscopy wikiPageRevisionID "694289913".
- Interferometric_scattering_microscopy wikiPageWikiLink Category:Microscopy.
- Interferometric_scattering_microscopy wikiPageWikiLink Dark_field_microscopy.
- Interferometric_scattering_microscopy wikiPageWikiLink Differential_interference_contrast_microscopy.
- Interferometric_scattering_microscopy wikiPageWikiLink Interface_(chemistry).
- Interferometric_scattering_microscopy wikiPageWikiLink Interference_reflection_microscopy.
- Interferometric_scattering_microscopy wikiPageWikiLink Reflection_(physics).
- Interferometric_scattering_microscopy wikiPageWikiLink Scattering.
- Interferometric_scattering_microscopy wikiPageWikiLink File:ISCAT_applications.gif.
- Interferometric_scattering_microscopy wikiPageWikiLink File:Principle_of_iSCAT_detection_and_basic_iSCAT_setup.png.
- Interferometric_scattering_microscopy wikiPageWikiLinkText "Interferometric scattering microscopy".
- Interferometric_scattering_microscopy wikiPageWikiLinkText "interferometric scattering microscopy".
- Interferometric_scattering_microscopy date "November 2015".
- Interferometric_scattering_microscopy source "ArticleWizard".
- Interferometric_scattering_microscopy wikiPageUsesTemplate Template:Orphan.
- Interferometric_scattering_microscopy wikiPageUsesTemplate Template:Reflist.
- Interferometric_scattering_microscopy wikiPageUsesTemplate Template:Userspace_draft.
- Interferometric_scattering_microscopy subject Category:Microscopy.
- Interferometric_scattering_microscopy hypernym Technique.
- Interferometric_scattering_microscopy type TopicalConcept.
- Interferometric_scattering_microscopy comment "Interferometric scattering microscopy (iSCAT) is an optical microscopy technique that relies on collecting light scattered by an object together with a reference light field, provided by the reflection at an interface. In the presence of the scattering object, the total intensity of light returning in the direction of the incident beam is different than in the absence of that object due to interference of the scattered and reflected light.".
- Interferometric_scattering_microscopy label "Interferometric scattering microscopy".
- Interferometric_scattering_microscopy wasDerivedFrom Interferometric_scattering_microscopy?oldid=694289913.
- Interferometric_scattering_microscopy depiction Principle_of_iSCAT_detection_and_basic_iSCAT_setup.png.
- Interferometric_scattering_microscopy isPrimaryTopicOf Interferometric_scattering_microscopy.