Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/High-performance_liquid_chromatography> ?p ?o }
- High-performance_liquid_chromatography abstract "High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.HPLC has been used for manufacturing (e.g. during the production process of pharmaceutical and biological products), legal (e.g. detecting performance enhancement drugs in urine), research (e.g. separating the components of a complex biological sample, or of similar synthetic chemicals from each other), and medical (e.g. detecting vitamin D levels in blood serum) purposes.Chromatography can be described as a mass transfer process involving adsorption. HPLC relies on pumps to pass a pressurized liquid and a sample mixture through a column filled with a sorbent, leading to the separation of the sample components. The active component of the column, the sorbent, is typically a granular material made of solid particles (e.g. silica, polymers, etc.), 2–50 micrometers in size. The components of the sample mixture are separated from each other due to their different degrees of interaction with the sorbent particles. The pressurized liquid is typically a mixture of solvents (e.g. water, acetonitrile and/or methanol) and is referred to as a \"mobile phase\". Its composition and temperature play a major role in the separation process by influencing the interactions taking place between sample components and sorbent. These interactions are physical in nature, such as hydrophobic (dispersive), dipole–dipole and ionic, most often a combination.HPLC is distinguished from traditional (\"low pressure\") liquid chromatography because operational pressures are significantly higher (50–350 bar), while ordinary liquid chromatography typically relies on the force of gravity to pass the mobile phase through the column. Due to the small sample amount separated in analytical HPLC, typical column dimensions are 2.1–4.6 mm diameter, and 30–250 mm length. Also HPLC columns are made with smaller sorbent particles (2–50 micrometer in average particle size). This gives HPLC superior resolving power (the ability to distinguish between compounds) when separating mixtures, which makes it a popular chromatographic technique.The schematic of an HPLC instrument typically includes a sampler, pumps, and a detector. The sampler brings the sample mixture into the mobile phase stream which carries it into the column. The pumps deliver the desired flow and composition of the mobile phase through the column. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor and user software control the HPLC instrument and provide data analysis. Some models of mechanical pumps in a HPLC instrument can mix multiple solvents together in ratios changing in time, generating a composition gradient in the mobile phase. Various detectors are in common use, such as UV/Vis, photodiode array (PDA) or based on mass spectrometry. Most HPLC instruments also have a column oven that allows for adjusting the temperature the separation is performed at.".
- High-performance_liquid_chromatography thumbnail Hplc.JPG?width=300.
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- High-performance_liquid_chromatography wikiPageWikiLink Acetic_acid.
- High-performance_liquid_chromatography wikiPageWikiLink Acetonitrile.
- High-performance_liquid_chromatography wikiPageWikiLink Acid.
- High-performance_liquid_chromatography wikiPageWikiLink Adduct.
- High-performance_liquid_chromatography wikiPageWikiLink Adsorption.
- High-performance_liquid_chromatography wikiPageWikiLink Aluminium_oxide.
- High-performance_liquid_chromatography wikiPageWikiLink Amino_acid.
- High-performance_liquid_chromatography wikiPageWikiLink Ammonium_formate.
- High-performance_liquid_chromatography wikiPageWikiLink Analytical_chemistry.
- High-performance_liquid_chromatography wikiPageWikiLink Anion-exchange_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Aqueous_normal-phase_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Archer_Martin.
- High-performance_liquid_chromatography wikiPageWikiLink Base_(chemistry).
- High-performance_liquid_chromatography wikiPageWikiLink Biomolecular_structure.
- High-performance_liquid_chromatography wikiPageWikiLink Biomolecule.
- High-performance_liquid_chromatography wikiPageWikiLink Biopolymer.
- High-performance_liquid_chromatography wikiPageWikiLink Bipyridine.
- High-performance_liquid_chromatography wikiPageWikiLink Buffering_agent.
- High-performance_liquid_chromatography wikiPageWikiLink Capillary_electrochromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Carboxylic_acid.
- High-performance_liquid_chromatography wikiPageWikiLink Category:Chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Category:Scientific_techniques.
- High-performance_liquid_chromatography wikiPageWikiLink Chelation.
- High-performance_liquid_chromatography wikiPageWikiLink Chloroform.
- High-performance_liquid_chromatography wikiPageWikiLink Chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Chromatography_detector.
- High-performance_liquid_chromatography wikiPageWikiLink Column_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Coulombs_law.
- High-performance_liquid_chromatography wikiPageWikiLink Countercurrent_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Counterion.
- High-performance_liquid_chromatography wikiPageWikiLink Csaba_Horváth_(chemical_engineer).
- High-performance_liquid_chromatography wikiPageWikiLink Dextran.
- High-performance_liquid_chromatography wikiPageWikiLink Displacement_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Elution.
- High-performance_liquid_chromatography wikiPageWikiLink Entropy.
- High-performance_liquid_chromatography wikiPageWikiLink Fluorescence.
- High-performance_liquid_chromatography wikiPageWikiLink Formic_acid.
- High-performance_liquid_chromatography wikiPageWikiLink Fused_quartz.
- High-performance_liquid_chromatography wikiPageWikiLink Gas_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Gradient.
- High-performance_liquid_chromatography wikiPageWikiLink Heparin.
- High-performance_liquid_chromatography wikiPageWikiLink High-performance_liquid_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink History_of_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Hydrogen_bond.
- High-performance_liquid_chromatography wikiPageWikiLink Hydrophilic_interaction_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Intermolecular_force.
- High-performance_liquid_chromatography wikiPageWikiLink Ion.
- High-performance_liquid_chromatography wikiPageWikiLink Ion_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Isopropyl_alcohol.
- High-performance_liquid_chromatography wikiPageWikiLink Joule.
- High-performance_liquid_chromatography wikiPageWikiLink Liquid_chromatography–mass_spectrometry.
- High-performance_liquid_chromatography wikiPageWikiLink Liquid–liquid_extraction.
- High-performance_liquid_chromatography wikiPageWikiLink Mass_spectrometry.
- High-performance_liquid_chromatography wikiPageWikiLink Mass_transfer.
- High-performance_liquid_chromatography wikiPageWikiLink Metal.
- High-performance_liquid_chromatography wikiPageWikiLink Methanol.
- High-performance_liquid_chromatography wikiPageWikiLink Micellar_liquid_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Microprocessor.
- High-performance_liquid_chromatography wikiPageWikiLink Mixture.
- High-performance_liquid_chromatography wikiPageWikiLink Nobel_Prize.
- High-performance_liquid_chromatography wikiPageWikiLink Organic_compound.
- High-performance_liquid_chromatography wikiPageWikiLink PH.
- High-performance_liquid_chromatography wikiPageWikiLink Paper_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Particle_technology.
- High-performance_liquid_chromatography wikiPageWikiLink Partition_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Partition_coefficient.
- High-performance_liquid_chromatography wikiPageWikiLink Phase_(matter).
- High-performance_liquid_chromatography wikiPageWikiLink Photodiode.
- High-performance_liquid_chromatography wikiPageWikiLink Polymer.
- High-performance_liquid_chromatography wikiPageWikiLink Pounds_per_square_inch.
- High-performance_liquid_chromatography wikiPageWikiLink Pressure.
- High-performance_liquid_chromatography wikiPageWikiLink Properties_of_water.
- High-performance_liquid_chromatography wikiPageWikiLink Pump.
- High-performance_liquid_chromatography wikiPageWikiLink Quantity.
- High-performance_liquid_chromatography wikiPageWikiLink Retardation_factor.
- High-performance_liquid_chromatography wikiPageWikiLink Reversed-phase_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Richard_Laurence_Millington_Synge.
- High-performance_liquid_chromatography wikiPageWikiLink Silicon_dioxide.
- High-performance_liquid_chromatography wikiPageWikiLink Size-exclusion_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Sodium_phosphates.
- High-performance_liquid_chromatography wikiPageWikiLink Solid_phase_extraction.
- High-performance_liquid_chromatography wikiPageWikiLink Solvent.
- High-performance_liquid_chromatography wikiPageWikiLink Solvophobic.
- High-performance_liquid_chromatography wikiPageWikiLink Spectrophotometry.
- High-performance_liquid_chromatography wikiPageWikiLink Steric_effects.
- High-performance_liquid_chromatography wikiPageWikiLink Stokes_radius.
- High-performance_liquid_chromatography wikiPageWikiLink Structural_isomer.
- High-performance_liquid_chromatography wikiPageWikiLink Surface_tension.
- High-performance_liquid_chromatography wikiPageWikiLink Temperature.
- High-performance_liquid_chromatography wikiPageWikiLink Tetrahydrofuran.
- High-performance_liquid_chromatography wikiPageWikiLink Thin-layer_chromatography.
- High-performance_liquid_chromatography wikiPageWikiLink Transfer_hydrogenation.
- High-performance_liquid_chromatography wikiPageWikiLink Trifluoroacetic_acid.