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- HNCA_experiment abstract "HNCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. The name derives from the experiment's magnetization transfer pathway: The magnetization of the amide proton of an amino acid residue is transferred to the amide nitrogen, and then to the alpha carbons of both the starting residue and the previous residue in the protein's amino acid sequence. In contrast, the complementary HNCOCA experimenttransfers magnetization only to the alpha carbon of the previous residue. The HNCA experiment is used, often in tandem with HNCOCA, to assign alpha carbon resonance signals to specific residues in the protein. This experiment requires a purified sample of protein prepared with 13C and 15N isotopic labelling, at a concentration greater than 0.1 mM, and is thus generally only applied to recombinant proteins.The spectrum produced by this experiment has 3 dimensions: A proton axis, a 15N axis and a 13C axis. For residue i peaks will appear at {HN(i), N(i), Calpha (i)} and {HN(i), N(i), Calpha(i-1)}, while for the complementary HNCOCA experiment peaks appear only at {HN(i), N(i), Calpha(i-1)}. Together, these two experiments reveal the alpha carbon chemical shift for each amino acid residue in a protein, and provide information linking adjacent residues in the protein's sequence.".
- HNCA_experiment thumbnail Hncahncoca.jpg?width=300.
- HNCA_experiment wikiPageID "3663969".
- HNCA_experiment wikiPageLength "2729".
- HNCA_experiment wikiPageOutDegree "16".
- HNCA_experiment wikiPageRevisionID "662684947".
- HNCA_experiment wikiPageWikiLink Amino_acid.
- HNCA_experiment wikiPageWikiLink Carbon-13.
- HNCA_experiment wikiPageWikiLink Category:Biophysics.
- HNCA_experiment wikiPageWikiLink Category:Nuclear_magnetic_resonance_experiments.
- HNCA_experiment wikiPageWikiLink Category:Protein_methods.
- HNCA_experiment wikiPageWikiLink Category:Protein_structure.
- HNCA_experiment wikiPageWikiLink Concentration.
- HNCA_experiment wikiPageWikiLink HNCOCA_experiment.
- HNCA_experiment wikiPageWikiLink Isotopes_of_nitrogen.
- HNCA_experiment wikiPageWikiLink Isotopic_labeling.
- HNCA_experiment wikiPageWikiLink Nuclear_magnetic_resonance.
- HNCA_experiment wikiPageWikiLink Nuclear_magnetic_resonance_spectroscopy_of_proteins.
- HNCA_experiment wikiPageWikiLink Recombinant_DNA.
- HNCA_experiment wikiPageWikiLink Triple-resonance_nuclear_magnetic_resonance_spectroscopy.
- HNCA_experiment wikiPageWikiLink File:Hncahncoca.jpg.
- HNCA_experiment wikiPageWikiLinkText "HNCA experiment".
- HNCA_experiment wikiPageWikiLinkText "HNCA".
- HNCA_experiment wikiPageUsesTemplate Template:Cite_journal.
- HNCA_experiment wikiPageUsesTemplate Template:Note.
- HNCA_experiment wikiPageUsesTemplate Template:Ref.
- HNCA_experiment subject Category:Biophysics.
- HNCA_experiment subject Category:Nuclear_magnetic_resonance_experiments.
- HNCA_experiment subject Category:Protein_methods.
- HNCA_experiment subject Category:Protein_structure.
- HNCA_experiment type Biophysic.
- HNCA_experiment type Method.
- HNCA_experiment type Physic.
- HNCA_experiment comment "HNCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. The name derives from the experiment's magnetization transfer pathway: The magnetization of the amide proton of an amino acid residue is transferred to the amide nitrogen, and then to the alpha carbons of both the starting residue and the previous residue in the protein's amino acid sequence.".
- HNCA_experiment label "HNCA experiment".
- HNCA_experiment sameAs Q5635300.
- HNCA_experiment sameAs m.09t3cy.
- HNCA_experiment sameAs Q5635300.
- HNCA_experiment wasDerivedFrom HNCA_experiment?oldid=662684947.
- HNCA_experiment depiction Hncahncoca.jpg.
- HNCA_experiment isPrimaryTopicOf HNCA_experiment.