Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/Gateway_Technology> ?p ?o }
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- Gateway_Technology abstract "The Gateway cloning System, invented and commercialized by Invitrogen since the late 1990s, is a molecular biology method that enables researchers to efficiently transfer DNA-fragments between plasmids using a proprietary set of recombination sequences, the \"Gateway att\" sites, and two proprietary enzyme mixes, called \"LR Clonase\", and \"BP Clonase\". Gateway Cloning Technique allows transfer of DNA fragments between different cloning vectors while maintaining the reading frame. Using Gateway, one can clone or subclone DNA segments for functional analysis. The system requires the initial insertion of a DNA fragment into a plasmid with two flanking recombination sequences called “att L 1” and “att L 2”, to develop a “Gateway Entry clone” (special Invitrogen nomenclature).Large archives of Gateway Entry clones, containing the vast majority of human, mouse and rat ORFs (open reading frames) have been cloned from human cDNA libraries or chemically synthesized to support the research community using NIH (National Institutes of Health) funding (e.g., Mammalian Gene Collection, http://mgc.nci.nih.gov/). The availability of these gene cassettes in a standard Gateway cloning plasmid helps researchers quickly transfer these cassettes into plasmids that facilitate the analysis of gene function. Gateway cloning does take more time for initial set-up, and is more expensive than traditional restriction enzyme and ligase-based cloning methods, but it saves time, and offers simpler and highly efficient cloning for down-stream applications.The technology has been widely adopted by the life science research community especially for applications that require the transfer of thousands of DNA fragments into one type of plasmid (e.g., one containing a CMV promoter for protein expression in mammalian cells), or for the transfer of one DNA fragment into many different types of plasmids (e.g., for bacterial, insect and mammalian protein expression).".
- Gateway_Technology wikiPageExternalLink pmidlookup?view=long&pmid=11076863.
- Gateway_Technology wikiPageExternalLink 17460441.2.4.571.
- Gateway_Technology wikiPageExternalLink S1046-5928(08)00047-8.
- Gateway_Technology wikiPageExternalLink )..
- Gateway_Technology wikiPageExternalLink Gateway-Cloning.html.
- Gateway_Technology wikiPageID "20579517".
- Gateway_Technology wikiPageLength "5755".
- Gateway_Technology wikiPageOutDegree "16".
- Gateway_Technology wikiPageRevisionID "700312451".
- Gateway_Technology wikiPageWikiLink Category:Molecular_biology.
- Gateway_Technology wikiPageWikiLink Cloning.
- Gateway_Technology wikiPageWikiLink Complementary_DNA.
- Gateway_Technology wikiPageWikiLink Gateway_cassette.
- Gateway_Technology wikiPageWikiLink Invitrogen.
- Gateway_Technology wikiPageWikiLink Merck_Group.
- Gateway_Technology wikiPageWikiLink National_Institutes_of_Health.
- Gateway_Technology wikiPageWikiLink Open_reading_frame.
- Gateway_Technology wikiPageWikiLink Plasmid.
- Gateway_Technology wikiPageWikiLink Polymerase_chain_reaction.
- Gateway_Technology wikiPageWikiLink Reading_frame.
- Gateway_Technology wikiPageWikiLink Subcloning.
- Gateway_Technology wikiPageWikiLink Vendor_lock-in.
- Gateway_Technology wikiPageWikiLinkText "Gateway Technology".
- Gateway_Technology wikiPageWikiLinkText "Gateway cloning system".
- Gateway_Technology wikiPageWikiLinkText "gateway method".
- Gateway_Technology wikiPageUsesTemplate Template:Cite_journal.
- Gateway_Technology wikiPageUsesTemplate Template:Reflist.
- Gateway_Technology subject Category:Molecular_biology.
- Gateway_Technology hypernym Method.
- Gateway_Technology type Software.
- Gateway_Technology comment "The Gateway cloning System, invented and commercialized by Invitrogen since the late 1990s, is a molecular biology method that enables researchers to efficiently transfer DNA-fragments between plasmids using a proprietary set of recombination sequences, the \"Gateway att\" sites, and two proprietary enzyme mixes, called \"LR Clonase\", and \"BP Clonase\". Gateway Cloning Technique allows transfer of DNA fragments between different cloning vectors while maintaining the reading frame.".
- Gateway_Technology label "Gateway Technology".
- Gateway_Technology sameAs Q5527296.
- Gateway_Technology sameAs Technologie_Gateway.
- Gateway_Technology sameAs m.052401m.
- Gateway_Technology sameAs Q5527296.
- Gateway_Technology sameAs 高通量基因克隆技术.
- Gateway_Technology wasDerivedFrom Gateway_Technology?oldid=700312451.
- Gateway_Technology isPrimaryTopicOf Gateway_Technology.