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- DyLight_Fluor abstract "The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific. DyLight dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology. Historically, fluorophores such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser, because they photobleach quickly (however, lifetimes can be increased at least 10 fold using oxygen scavenging). DyLight Fluors have comparable excitation and emission spectra and are claimed to be more photostable, brighter, and less pH-sensitive. The excitation and emission spectra of the DyLight Fluor series covers much of the visible spectrum and extends into the infrared region, allowing detection using most fluorescence microscopes, as well as infrared imaging systems.To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.DyLight Fluors are synthesized through sulfonate addition to coumarin, xanthene (such as fluorescein and rhodamine), and cyanine dyes. Sulfonation makes DyLight dyes negatively charged and hydrophilic. DyLight Fluors are commercially available as reactive succinimidyl-esters for labeling proteins through lysine residues, and as maleimide derivatives for labeling proteins through cysteine residues. Antibodies conjugated are also available from several companies. Similar lines of fluorescent dyes provide an alternative to the DyLight Dyes (see also the list in Category:Fluorescent dyes).".
- DyLight_Fluor wikiPageID "10801190".
- DyLight_Fluor wikiPageLength "4412".
- DyLight_Fluor wikiPageOutDegree "31".
- DyLight_Fluor wikiPageRevisionID "646852365".
- DyLight_Fluor wikiPageWikiLink Amino_acid.
- DyLight_Fluor wikiPageWikiLink Biomolecule.
- DyLight_Fluor wikiPageWikiLink Category:Dyes.
- DyLight_Fluor wikiPageWikiLink Category:Fluorescent_dyes.
- DyLight_Fluor wikiPageWikiLink Cell_biology.
- DyLight_Fluor wikiPageWikiLink Chemical_synthesis.
- DyLight_Fluor wikiPageWikiLink Coumarin.
- DyLight_Fluor wikiPageWikiLink Cyanine.
- DyLight_Fluor wikiPageWikiLink Cysteine.
- DyLight_Fluor wikiPageWikiLink Dyomics.
- DyLight_Fluor wikiPageWikiLink Electric_charge.
- DyLight_Fluor wikiPageWikiLink Emission_spectrum.
- DyLight_Fluor wikiPageWikiLink Excited_state.
- DyLight_Fluor wikiPageWikiLink Fluorescein.
- DyLight_Fluor wikiPageWikiLink Fluorescence.
- DyLight_Fluor wikiPageWikiLink Fluorescence_microscope.
- DyLight_Fluor wikiPageWikiLink Fluorophore.
- DyLight_Fluor wikiPageWikiLink Hydrophile.
- DyLight_Fluor wikiPageWikiLink Infrared.
- DyLight_Fluor wikiPageWikiLink Laser_diode.
- DyLight_Fluor wikiPageWikiLink Lysine.
- DyLight_Fluor wikiPageWikiLink Maleimide.
- DyLight_Fluor wikiPageWikiLink Molecular_biology.
- DyLight_Fluor wikiPageWikiLink PH.
- DyLight_Fluor wikiPageWikiLink Photobleaching.
- DyLight_Fluor wikiPageWikiLink Rhodamine.
- DyLight_Fluor wikiPageWikiLink Sulfonate.
- DyLight_Fluor wikiPageWikiLink Thermo_Fisher_Scientific.
- DyLight_Fluor wikiPageWikiLink Visible_spectrum.
- DyLight_Fluor wikiPageWikiLink Xanthene.
- DyLight_Fluor wikiPageWikiLinkText "DyLight 488".
- DyLight_Fluor wikiPageWikiLinkText "DyLight 550 and DyLight 633".
- DyLight_Fluor wikiPageWikiLinkText "DyLight 594".
- DyLight_Fluor wikiPageWikiLinkText "DyLight Fluor".
- DyLight_Fluor wikiPageWikiLinkText "DyLight".
- DyLight_Fluor wikiPageWikiLinkText "Dylight".
- DyLight_Fluor wikiPageUsesTemplate Template:Reflist.
- DyLight_Fluor subject Category:Dyes.
- DyLight_Fluor subject Category:Fluorescent_dyes.
- DyLight_Fluor type Redirect.
- DyLight_Fluor type Textile.
- DyLight_Fluor comment "The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific. DyLight dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology. Historically, fluorophores such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications.".
- DyLight_Fluor label "DyLight Fluor".
- DyLight_Fluor sameAs Q5318380.
- DyLight_Fluor sameAs DyLight_Fluor.
- DyLight_Fluor sameAs m.02qqg8m.
- DyLight_Fluor sameAs Q5318380.
- DyLight_Fluor sameAs DyLight_Fluor.
- DyLight_Fluor wasDerivedFrom DyLight_Fluor?oldid=646852365.
- DyLight_Fluor isPrimaryTopicOf DyLight_Fluor.