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- Duplex_sequencing abstract "Duplex sequencing is a library preparation and analysis method for next-generation sequencing (NGS) platforms that employs random tagging of double stranded DNA to detect mutations with higher accuracy and lower error rate. This method uses degenerate molecular tags in addition to sequencing adapters to recognize reads originating from each strand of DNA. The generated sequencing reads then will be analyzed using two methods: single strand consensus sequences (SSCSs) and Duplex consensus sequences (DCSs) assembly. Duplex sequencing theoretically can detect mutations with frequencies as low as 5 x 10−8 that is more than 10,000 fold higher in accuracy compared to the conventional next-generation sequencing methods.The estimated error rate of standard next-generation sequencing platforms is 10−2 - 10−3 per base call. With this error rate billions of base calls that are produced by NGS will results in millions of the errors. The errors are introduced during sample preparation and sequencing such as polymerase , sequencing and image analysis errors. While the NGS platforms error rate is admissible to some applications such as detection of clonal variants, it is a major limit for applications that require higher accuracy for detection of low frequency variants such as detection of intra-organismal mosaicism, subclonal variants in genetically heterogeneous cancers or circulating tumor DNA.Several techniques have been developed that increase accuracy of NGS platforms such as barcoding techniques and circle sequencing method. The data generated by these methods the same as NGS platforms originate from single strand of DNA and therefore the errors that are introduced during PCR amplification, tissue processing or DNA extraction can still be distinguished as a true variant. Duplex sequencing method solve this problem by taking advantage of complementary nature of two strands of DNA and confirming only variants that are present in both strands of DNA. Since the probability of two errors happening at the same exact base pair in both strands and resulting in a complementary basepair is very low, duplex sequencing increases the accuracy of sequencing significantly.".
- Duplex_sequencing thumbnail Duplex_sequencing_overview.svg?width=300.
- Duplex_sequencing wikiPageID "49528672".
- Duplex_sequencing wikiPageLength "18131".
- Duplex_sequencing wikiPageOutDegree "45".
- Duplex_sequencing wikiPageRevisionID "708319244".
- Duplex_sequencing wikiPageWikiLink Cancer_genome_sequencing.
- Duplex_sequencing wikiPageWikiLink Category:Bioinformatics.
- Duplex_sequencing wikiPageWikiLink Category:DNA.
- Duplex_sequencing wikiPageWikiLink Category:Emerging_technologies.
- Duplex_sequencing wikiPageWikiLink Category:Gene_tests.
- Duplex_sequencing wikiPageWikiLink Category:Genomics.
- Duplex_sequencing wikiPageWikiLink Category:Medical_genetics.
- Duplex_sequencing wikiPageWikiLink Category:Molecular_biology.
- Duplex_sequencing wikiPageWikiLink Category:Molecular_genetics.
- Duplex_sequencing wikiPageWikiLink Consensus_sequence.
- Duplex_sequencing wikiPageWikiLink Copy-number_variation.
- Duplex_sequencing wikiPageWikiLink DNA.
- Duplex_sequencing wikiPageWikiLink DNA_extraction.
- Duplex_sequencing wikiPageWikiLink DNA_fragmentation.
- Duplex_sequencing wikiPageWikiLink DNA_polymerase.
- Duplex_sequencing wikiPageWikiLink DNA_sequencing.
- Duplex_sequencing wikiPageWikiLink Gel_electrophoresis_of_nucleic_acids.
- Duplex_sequencing wikiPageWikiLink Histology.
- Duplex_sequencing wikiPageWikiLink Library_(biology).
- Duplex_sequencing wikiPageWikiLink Ligation_(molecular_biology).
- Duplex_sequencing wikiPageWikiLink List_of_restriction_enzyme_cutting_sites:_G–K.
- Duplex_sequencing wikiPageWikiLink List_of_sequence_alignment_software.
- Duplex_sequencing wikiPageWikiLink Mosaic_(genetics).
- Duplex_sequencing wikiPageWikiLink Nucleic_acid_notation.
- Duplex_sequencing wikiPageWikiLink Nucleic_acid_thermodynamics.
- Duplex_sequencing wikiPageWikiLink Oligonucleotide.
- Duplex_sequencing wikiPageWikiLink Personalized_medicine.
- Duplex_sequencing wikiPageWikiLink Point_mutation.
- Duplex_sequencing wikiPageWikiLink Polymerase.
- Duplex_sequencing wikiPageWikiLink Polymerase_chain_reaction.
- Duplex_sequencing wikiPageWikiLink Prenatal_diagnosis.
- Duplex_sequencing wikiPageWikiLink Reference_genome.
- Duplex_sequencing wikiPageWikiLink Restriction_enzyme.
- Duplex_sequencing wikiPageWikiLink Sequence_alignment.
- Duplex_sequencing wikiPageWikiLink Somatic_evolution_in_cancer.
- Duplex_sequencing wikiPageWikiLink Sonication.
- Duplex_sequencing wikiPageWikiLink Transversion.
- Duplex_sequencing wikiPageWikiLink Tumour_heterogeneity.
- Duplex_sequencing wikiPageWikiLink Upstream_and_downstream_(DNA).
- Duplex_sequencing wikiPageWikiLink File:Duplex_sequencing_library_preparation_procedure.png.
- Duplex_sequencing wikiPageWikiLink File:Duplex_sequencing_overview.svg.
- Duplex_sequencing wikiPageWikiLinkText "Duplex sequencing".
- Duplex_sequencing wikiPageUsesTemplate Template:Col-2.
- Duplex_sequencing wikiPageUsesTemplate Template:Col-begin.
- Duplex_sequencing wikiPageUsesTemplate Template:Col-end.
- Duplex_sequencing wikiPageUsesTemplate Template:Reflist.
- Duplex_sequencing subject Category:Bioinformatics.
- Duplex_sequencing subject Category:DNA.
- Duplex_sequencing subject Category:Emerging_technologies.
- Duplex_sequencing subject Category:Gene_tests.
- Duplex_sequencing subject Category:Genomics.
- Duplex_sequencing subject Category:Medical_genetics.
- Duplex_sequencing subject Category:Molecular_biology.
- Duplex_sequencing subject Category:Molecular_genetics.
- Duplex_sequencing hypernym Preparation.
- Duplex_sequencing type Food.
- Duplex_sequencing comment "Duplex sequencing is a library preparation and analysis method for next-generation sequencing (NGS) platforms that employs random tagging of double stranded DNA to detect mutations with higher accuracy and lower error rate. This method uses degenerate molecular tags in addition to sequencing adapters to recognize reads originating from each strand of DNA.".
- Duplex_sequencing label "Duplex sequencing".
- Duplex_sequencing wasDerivedFrom Duplex_sequencing?oldid=708319244.
- Duplex_sequencing depiction Duplex_sequencing_overview.svg.
- Duplex_sequencing isPrimaryTopicOf Duplex_sequencing.