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- Dot_blot abstract "A dot blot (or slot blot) is a technique in molecular biology used to detect biomolecules, and for detecting, analyzing, and identifying proteins. It represents a simplification of the northern blot, Southern blot, or western blot methods. In a dot blot the biomolecules to be detected are not first separated by electrophoresis. Instead, a mixture containing the molecule to be detected is applied directly on a membrane as a dot, and then is spotted through circular templates directly onto the membrane or paper substrate. This differs from the western blot because protein samples are not separated electrophoretically. This is then followed by detection by either nucleotide probes (for a northern blot and southern blot) or antibodies (for a western blot).The technique offers significant savings in time, as chromatography or gel electrophoresis, and the complex blotting procedures for the gel are not required. However, it offers no information on the size of the target biomolecule. Furthermore, if two molecules of different sizes are detected, they will still appear as a single dot. Dot blots therefore can only confirm the presence or absence of a biomolecule or biomolecules which can be detected by the DNA probes or the antibody.Because dot blot does not require complicated instrument to operate, lots of dot blot assays are developed using antibodies with high specificity to detect different protein targets. Dot blot is also used to evaluate or screen the effectiveness of the antibodies.".
- Dot_blot thumbnail DotBlotDemo.jpg?width=300.
- Dot_blot wikiPageExternalLink 312.full.
- Dot_blot wikiPageExternalLink www.Vitrozm.com.
- Dot_blot wikiPageExternalLink Dot%20blot%20protocol.pdf.
- Dot_blot wikiPageExternalLink M1706542C.pdf.
- Dot_blot wikiPageExternalLink index.asp?topic_id=144.
- Dot_blot wikiPageExternalLink index.php?option=com_content&task=view&id=16&Itemid=9.
- Dot_blot wikiPageExternalLink PMC95755.
- Dot_blot wikiPageExternalLink zoom-blot-fast-and-easy-96-well-dot-blot.
- Dot_blot wikiPageID "4128491".
- Dot_blot wikiPageLength "5753".
- Dot_blot wikiPageOutDegree "34".
- Dot_blot wikiPageRevisionID "697140482".
- Dot_blot wikiPageWikiLink Antibody.
- Dot_blot wikiPageWikiLink Biomolecule.
- Dot_blot wikiPageWikiLink Category:Molecular_biology.
- Dot_blot wikiPageWikiLink Chlamydia_trachomatis.
- Dot_blot wikiPageWikiLink Chromatography.
- Dot_blot wikiPageWikiLink Electrophoresis.
- Dot_blot wikiPageWikiLink File:DotBlotDemo.jpg.
- Dot_blot wikiPageWikiLink Gel_electrophoresis.
- Dot_blot wikiPageWikiLink Hybridization_probe.
- Dot_blot wikiPageWikiLink Molecular_biology.
- Dot_blot wikiPageWikiLink Molecule.
- Dot_blot wikiPageWikiLink Northern_blot.
- Dot_blot wikiPageWikiLink Nucleotide.
- Dot_blot wikiPageWikiLink Sexually_transmitted_infection.
- Dot_blot wikiPageWikiLink Southern_blot.
- Dot_blot wikiPageWikiLink Southwestern_blot.
- Dot_blot wikiPageWikiLink Tuberculosis.
- Dot_blot wikiPageWikiLink Typhoid_fever.
- Dot_blot wikiPageWikiLink Western_blot.
- Dot_blot wikiPageWikiLinkText "Dot blot".
- Dot_blot wikiPageWikiLinkText "dot blot".
- Dot_blot wikiPageWikiLinkText "dot-blot".
- Dot_blot class "noprint Template-Fact".
- Dot_blot link "Wikipedia:Citation needed".
- Dot_blot text "citation needed".
- Dot_blot title "This claim needs references to reliable sources".
- Dot_blot wikiPageUsesTemplate Template:Fix.
- Dot_blot wikiPageUsesTemplate Template:No_footnotes.
- Dot_blot subject Category:Molecular_biology.
- Dot_blot hypernym Technique.
- Dot_blot type TopicalConcept.
- Dot_blot comment "A dot blot (or slot blot) is a technique in molecular biology used to detect biomolecules, and for detecting, analyzing, and identifying proteins. It represents a simplification of the northern blot, Southern blot, or western blot methods. In a dot blot the biomolecules to be detected are not first separated by electrophoresis.".
- Dot_blot label "Dot blot".
- Dot_blot sameAs Q2344000.
- Dot_blot sameAs Dot_Blot.
- Dot_blot sameAs Dot_Blot.
- Dot_blot sameAs Dot_blot.
- Dot_blot sameAs Dot_blot.
- Dot_blot sameAs Dot_blot.
- Dot_blot sameAs m.0bkm26.
- Dot_blot sameAs Dot_blot.
- Dot_blot sameAs Q2344000.
- Dot_blot wasDerivedFrom Dot_blot?oldid=697140482.
- Dot_blot depiction DotBlotDemo.jpg.
- Dot_blot isPrimaryTopicOf Dot_blot.