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- Diagnostic_electron_microscopy abstract "The transmission electron microscope (TEM) is used as an important diagnostic tool to screen human tissues at high magnification (the ultrastructural level), often in conjunction with other methods, particularly light microscopy and immunofluorescence techniques. The TEM was first used extensively for this purpose in the 1980s, especially for identifying the markers of cell differentiation to identify tumours, and in renal disease. Immunolabelling techniques are now generally used instead of the TEM for tumour diagnosis but the technique retains a critical role in the diagnosis of renal disease and a range of other conditions. Specifically, TEM should be used for diagnostic purposes when it: (1) provides useful (complementary) information in the context of a carefully considered differential diagnosis; (2) provides an ‘improved’ diagnosis that results in better treatment strategies and; (3) is time & cost effective in respect to alternative techniques. For diagnostic purposes solid tissues are prepared for TEM in the same way as other biological tissues, they are fixed in glutaraldehyde and osmium tetroxide then dehydrated and embedded in epoxy resin. The epoxy resin block is trimmed and the target tissue is selected using a light microscope by viewing semithin sections stained with toluidine blue. The block is then retrimmed and the specific area for observation is ultrathin sectioned, preferably using a diamond knife. The ultrathin sections are collected on 3mm copper (mesh) grids and stained with uranyl acetate and lead citrate to make the contents of the tissue electron dense (and thus visible in the electron microscope).".
- Diagnostic_electron_microscopy wikiPageExternalLink www.acem.org.uk.
- Diagnostic_electron_microscopy wikiPageID "20288037".
- Diagnostic_electron_microscopy wikiPageLength "2221".
- Diagnostic_electron_microscopy wikiPageOutDegree "13".
- Diagnostic_electron_microscopy wikiPageRevisionID "400643973".
- Diagnostic_electron_microscopy wikiPageWikiLink Category:Microscopy.
- Diagnostic_electron_microscopy wikiPageWikiLink Cellular_differentiation.
- Diagnostic_electron_microscopy wikiPageWikiLink Cost-effectiveness_analysis.
- Diagnostic_electron_microscopy wikiPageWikiLink Diagnosis.
- Diagnostic_electron_microscopy wikiPageWikiLink Differential_diagnosis.
- Diagnostic_electron_microscopy wikiPageWikiLink Electron_microscope.
- Diagnostic_electron_microscopy wikiPageWikiLink Epoxy.
- Diagnostic_electron_microscopy wikiPageWikiLink Kidney.
- Diagnostic_electron_microscopy wikiPageWikiLink Microscopy.
- Diagnostic_electron_microscopy wikiPageWikiLink Optical_microscope.
- Diagnostic_electron_microscopy wikiPageWikiLink Osmium_tetroxide.
- Diagnostic_electron_microscopy wikiPageWikiLink Transmission_electron_microscopy.
- Diagnostic_electron_microscopy wikiPageWikiLink Uranyl_acetate.
- Diagnostic_electron_microscopy wikiPageWikiLinkText "Diagnostic electron microscopy".
- Diagnostic_electron_microscopy wikiPageUsesTemplate Template:Reflist.
- Diagnostic_electron_microscopy subject Category:Microscopy.
- Diagnostic_electron_microscopy type Technique.
- Diagnostic_electron_microscopy comment "The transmission electron microscope (TEM) is used as an important diagnostic tool to screen human tissues at high magnification (the ultrastructural level), often in conjunction with other methods, particularly light microscopy and immunofluorescence techniques. The TEM was first used extensively for this purpose in the 1980s, especially for identifying the markers of cell differentiation to identify tumours, and in renal disease.".
- Diagnostic_electron_microscopy label "Diagnostic electron microscopy".
- Diagnostic_electron_microscopy sameAs Q5270347.
- Diagnostic_electron_microscopy sameAs m.04zy_p8.
- Diagnostic_electron_microscopy sameAs Q5270347.
- Diagnostic_electron_microscopy wasDerivedFrom Diagnostic_electron_microscopy?oldid=400643973.
- Diagnostic_electron_microscopy isPrimaryTopicOf Diagnostic_electron_microscopy.