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- Chelex_100 abstract "Chelex® 100 is a chelating material from Bio-Rad used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions.It is a styrene-divinylbenzene co-polymer containing iminodiacetic acid groups.A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups.Chelex resin is often used for DNA extraction in preparation for PCR. The Chelex™ protects the sample from DNAases that might remain active after the boiling and could subsequently destroy the DNA. DNAases are enzymes, which naturally occur in all body tissues, they cut DNA into small fragments, rendering it unsuitable for PCR. Magnesium ions are essential cofactors for DNAases. Chelex™ resin binds with cations including Mg2+. By binding with magnesium ions, the Chelex™ resin renders DNAases inactive, thus protecting DNA from their action. After boiling the Chelex™ DNA preparation is stable and can be stored at 4oC for 3-4 months. Polar resin beads bind polar cellular components after breaking open cells, while DNA and RNA remain in water solution above chelex.It is also known that, after DNA is isolated from a cell, it is susceptible to DNases. However, the enzymes that degrade DNA require the cofactor Mg2+; based on this fact, Chelex tightly binds to all the Mg2+ in the extract to prevent DNA degradation. However, the heating steps do denature the double helix, and the resulting single-stranded DNA is less stable in storage.Chelex® is a registered trademark of Bio-Rad Laboratories, Inc.".
- Chelex_100 wikiPageExternalLink biorad?cmd=BRCatgProductDetail&productID=216601.
- Chelex_100 wikiPageID "13830976".
- Chelex_100 wikiPageLength "2735".
- Chelex_100 wikiPageOutDegree "16".
- Chelex_100 wikiPageRevisionID "698371360".
- Chelex_100 wikiPageWikiLink Bio-Rad_Laboratories.
- Chelex_100 wikiPageWikiLink Category:Chelating_agents.
- Chelex_100 wikiPageWikiLink Chelation.
- Chelex_100 wikiPageWikiLink Copolymer.
- Chelex_100 wikiPageWikiLink DNA.
- Chelex_100 wikiPageWikiLink DNA_extraction.
- Chelex_100 wikiPageWikiLink Deoxyribonuclease.
- Chelex_100 wikiPageWikiLink Iminodiacetic_acid.
- Chelex_100 wikiPageWikiLink Ion_exchange.
- Chelex_100 wikiPageWikiLink Magnesium.
- Chelex_100 wikiPageWikiLink Molar_concentration.
- Chelex_100 wikiPageWikiLink Nitric_acid.
- Chelex_100 wikiPageWikiLink Polymerase_chain_reaction.
- Chelex_100 wikiPageWikiLink Resin.
- Chelex_100 wikiPageWikiLink Styrene.
- Chelex_100 wikiPageWikiLink Transition_metal.
- Chelex_100 wikiPageWikiLinkText "Chelex 100".
- Chelex_100 wikiPageWikiLinkText "chelex 100".
- Chelex_100 wikiPageUsesTemplate Template:Chemistry-stub.
- Chelex_100 wikiPageUsesTemplate Template:Cite_journal.
- Chelex_100 wikiPageUsesTemplate Template:Reflist.
- Chelex_100 subject Category:Chelating_agents.
- Chelex_100 hypernym Material.
- Chelex_100 type Agent.
- Chelex_100 type ChemicalCompound.
- Chelex_100 type Agent.
- Chelex_100 type Chemical.
- Chelex_100 comment "Chelex® 100 is a chelating material from Bio-Rad used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions.It is a styrene-divinylbenzene co-polymer containing iminodiacetic acid groups.A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups.Chelex resin is often used for DNA extraction in preparation for PCR.".
- Chelex_100 label "Chelex 100".
- Chelex_100 sameAs Q5089854.
- Chelex_100 sameAs m.03ckl9g.
- Chelex_100 sameAs Chelex_100.
- Chelex_100 sameAs Q5089854.
- Chelex_100 wasDerivedFrom Chelex_100?oldid=698371360.
- Chelex_100 homepage biorad?cmd=BRCatgProductDetail&productID=216601.
- Chelex_100 isPrimaryTopicOf Chelex_100.