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- Cell-cycle_analysis abstract "Cell-cycle analysis is a method in cell biology that employs flow cytometry to distinguish cells in different phases of the cell cycle. Before analysis, the cells are permeabilised and treated with a fluorescent dye that stains DNA quantitatively, usually propidium iodide (PI). The fluorescence intensity of the stained cells at certain wavelengths will therefore correlate with the amount of DNA they contain. As the DNA content of cells duplicates during the S phase of the cell cycle, the relative amount of cells in the G0 phase and G1 phase (before S phase), in the S phase, and in the G2 phase and M phase (after S phase) can be determined, as the fluorescence of cells in the G2/M phase will be twice as high as that of cells in the G0/G1 phase.Cell-cycle anomalies can be symptoms for various kinds of cell damage, for example DNA damage, which cause the cell to interrupt the cell cycle at certain checkpoints to prevent transformation into a cancer cell (carcinogenesis). Other possible reasons for anomalies include lack of nutrients, for example after serum deprivation.Cell cycle analysis was first described in 1969 at Los Alamos Scientific Laboratory by a group from the University of California, using the Feulgen staining technique. The first protocol for cell-cycle analysis using propidium iodide staining was presented in 1975 by Awtar Krishan from Harvard Medical School and is still widely cited today.".
- Cell-cycle_analysis thumbnail 1D30_DNA_DAPI.png?width=300.
- Cell-cycle_analysis wikiPageExternalLink cell-division.
- Cell-cycle_analysis wikiPageExternalLink Introduction%20to%20Cell%20Cycle%20Analysis.pdf.
- Cell-cycle_analysis wikiPageExternalLink cellcyc.pdf.
- Cell-cycle_analysis wikiPageID "27382300".
- Cell-cycle_analysis wikiPageLength "8330".
- Cell-cycle_analysis wikiPageOutDegree "49".
- Cell-cycle_analysis wikiPageRevisionID "591455421".
- Cell-cycle_analysis wikiPageWikiLink 7-Aminoactinomycin_D.
- Cell-cycle_analysis wikiPageWikiLink Apoptosis.
- Cell-cycle_analysis wikiPageWikiLink Apoptotic_DNA_fragmentation.
- Cell-cycle_analysis wikiPageWikiLink Artifact_(error).
- Cell-cycle_analysis wikiPageWikiLink Awtar_Krishan.
- Cell-cycle_analysis wikiPageWikiLink Bisbenzimide.
- Cell-cycle_analysis wikiPageWikiLink Buffer_solution.
- Cell-cycle_analysis wikiPageWikiLink Carcinogenesis.
- Cell-cycle_analysis wikiPageWikiLink Category:Biological_techniques_and_tools.
- Cell-cycle_analysis wikiPageWikiLink Category:Cell_biology.
- Cell-cycle_analysis wikiPageWikiLink Category:Cell_cycle.
- Cell-cycle_analysis wikiPageWikiLink Category:Flow_cytometry.
- Cell-cycle_analysis wikiPageWikiLink Cell_biology.
- Cell-cycle_analysis wikiPageWikiLink Cell_cycle.
- Cell-cycle_analysis wikiPageWikiLink Cell_cycle_checkpoint.
- Cell-cycle_analysis wikiPageWikiLink Cell_membrane.
- Cell-cycle_analysis wikiPageWikiLink Cell_nucleus.
- Cell-cycle_analysis wikiPageWikiLink DAPI.
- Cell-cycle_analysis wikiPageWikiLink DNA.
- Cell-cycle_analysis wikiPageWikiLink DNA_repair.
- Cell-cycle_analysis wikiPageWikiLink Detergent.
- Cell-cycle_analysis wikiPageWikiLink Endonuclease.
- Cell-cycle_analysis wikiPageWikiLink Ethanol.
- Cell-cycle_analysis wikiPageWikiLink False_positives_and_false_negatives.
- Cell-cycle_analysis wikiPageWikiLink Feulgen_stain.
- Cell-cycle_analysis wikiPageWikiLink Fixation_(histology).
- Cell-cycle_analysis wikiPageWikiLink Flow_cytometry.
- Cell-cycle_analysis wikiPageWikiLink Fluorophore.
- Cell-cycle_analysis wikiPageWikiLink G0_phase.
- Cell-cycle_analysis wikiPageWikiLink G1_phase.
- Cell-cycle_analysis wikiPageWikiLink G2_phase.
- Cell-cycle_analysis wikiPageWikiLink Harvard_Medical_School.
- Cell-cycle_analysis wikiPageWikiLink Histogram.
- Cell-cycle_analysis wikiPageWikiLink Ildo_Nicoletti.
- Cell-cycle_analysis wikiPageWikiLink Laser.
- Cell-cycle_analysis wikiPageWikiLink Los_Alamos_National_Laboratory.
- Cell-cycle_analysis wikiPageWikiLink NP-40.
- Cell-cycle_analysis wikiPageWikiLink Pancreatic_ribonuclease.
- Cell-cycle_analysis wikiPageWikiLink Programmed_cell_death.
- Cell-cycle_analysis wikiPageWikiLink Propidium_iodide.
- Cell-cycle_analysis wikiPageWikiLink RNA.
- Cell-cycle_analysis wikiPageWikiLink S_phase.
- Cell-cycle_analysis wikiPageWikiLink Serum_(blood).
- Cell-cycle_analysis wikiPageWikiLink Triton_X-100.
- Cell-cycle_analysis wikiPageWikiLink University_of_California.
- Cell-cycle_analysis wikiPageWikiLink University_of_Perugia.
- Cell-cycle_analysis wikiPageWikiLink Wiktionary:Permeabilization.
- Cell-cycle_analysis wikiPageWikiLink File:1D30_DNA_DAPI.png.
- Cell-cycle_analysis wikiPageWikiLinkText "Cell-cycle analysis".
- Cell-cycle_analysis wikiPageWikiLinkText "Cell-cycle analysis#Nicoletti assay".
- Cell-cycle_analysis wikiPageUsesTemplate Template:Cite_web.
- Cell-cycle_analysis wikiPageUsesTemplate Template:Gallery.
- Cell-cycle_analysis wikiPageUsesTemplate Template:PDB.
- Cell-cycle_analysis wikiPageUsesTemplate Template:Reflist.
- Cell-cycle_analysis subject Category:Biological_techniques_and_tools.
- Cell-cycle_analysis subject Category:Cell_biology.
- Cell-cycle_analysis subject Category:Cell_cycle.
- Cell-cycle_analysis subject Category:Flow_cytometry.
- Cell-cycle_analysis hypernym Method.
- Cell-cycle_analysis type Software.
- Cell-cycle_analysis type Technique.
- Cell-cycle_analysis comment "Cell-cycle analysis is a method in cell biology that employs flow cytometry to distinguish cells in different phases of the cell cycle. Before analysis, the cells are permeabilised and treated with a fluorescent dye that stains DNA quantitatively, usually propidium iodide (PI). The fluorescence intensity of the stained cells at certain wavelengths will therefore correlate with the amount of DNA they contain.".
- Cell-cycle_analysis label "Cell-cycle analysis".
- Cell-cycle_analysis sameAs Q5058181.
- Cell-cycle_analysis sameAs Analýza_buněčného_cyklu.
- Cell-cycle_analysis sameAs m.0bxzylj.
- Cell-cycle_analysis sameAs Q5058181.
- Cell-cycle_analysis wasDerivedFrom Cell-cycle_analysis?oldid=591455421.
- Cell-cycle_analysis depiction 1D30_DNA_DAPI.png.
- Cell-cycle_analysis isPrimaryTopicOf Cell-cycle_analysis.