Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/Cas9> ?p ?o }
Showing triples 1 to 81 of
81
with 100 triples per page.
- Cas9 abstract "Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorize and later interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking whether it is complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes.Apart from its original function in bacterial immunity, the Cas9 protein has been heavily utilized as a genome engineering tool to induce site-directed double strand breaks in DNA. These breaks can lead to gene inactivation or the introduction of heterologous genes through non-homologous end joining and homologous recombination respectively in many laboratory model organisms. Alongside zinc finger nucleases and TALEN proteins, Cas9 is becoming a prominent tool in the field of genome editing.Cas9 has gained traction in recent years because it can cleave nearly any sequence complementary to the guide RNA. Because the target specificity of Cas9 stems from the guide RNA:DNA complementarity and not modifications to the protein itself (like TALENs and Zinc-fingers), engineering Cas9 to target new DNA is straightforward. Versions of Cas9 that bind but do not cleave cognate DNA can be used to localize transcriptional activator or repressors to specific DNA sequences in order to control transcriptional activation and repression. While native Cas9 requires a guide RNA composed of two disparate RNAs that associate to make the guide - the CRISPR RNA (crRNA), and the trans-activating RNA (tracrRNA)., Cas9 targeting has been simplified through the engineering of a chimeric single guide RNA. Scientists have suggested that Cas9-based gene drives may be capable of editing the genomes of entire populations of organisms. In 2015, scientists used Cas9 to modify the genome of human embryos for the first time.".
- Cas9 thumbnail Crystal_Structure_of_Cas9_in_Complex_with_Guide_RNA_and_Target_DNA.jpg?width=300.
- Cas9 wikiPageExternalLink default.tpl?cart=14520192576517890&action=full&--eqskudatarq=1073.
- Cas9 wikiPageID "38570862".
- Cas9 wikiPageLength "27293".
- Cas9 wikiPageOutDegree "45".
- Cas9 wikiPageRevisionID "700778764".
- Cas9 wikiPageWikiLink Activator_(genetics).
- Cas9 wikiPageWikiLink Adaptation.
- Cas9 wikiPageWikiLink Alanine.
- Cas9 wikiPageWikiLink Bacteriophage.
- Cas9 wikiPageWikiLink BioBrick.
- Cas9 wikiPageWikiLink CRISPR.
- Cas9 wikiPageWikiLink Cpf1.
- Cas9 wikiPageWikiLink Catalysis.
- Cas9 wikiPageWikiLink Category:Enzymes.
- Cas9 wikiPageWikiLink Category:Genetic_engineering.
- Cas9 wikiPageWikiLink Category:Repetitive_DNA_sequences.
- Cas9 wikiPageWikiLink Chromatin.
- Cas9 wikiPageWikiLink DNA.
- Cas9 wikiPageWikiLink Endonuclease.
- Cas9 wikiPageWikiLink Enzyme.
- Cas9 wikiPageWikiLink FokI.
- Cas9 wikiPageWikiLink Gene_drive.
- Cas9 wikiPageWikiLink Genome.
- Cas9 wikiPageWikiLink Genome_editing.
- Cas9 wikiPageWikiLink Heterochromatin.
- Cas9 wikiPageWikiLink Induced_stem_cells.
- Cas9 wikiPageWikiLink Locus_(genetics).
- Cas9 wikiPageWikiLink Molar_concentration.
- Cas9 wikiPageWikiLink Molecular_machine.
- Cas9 wikiPageWikiLink Plasmid.
- Cas9 wikiPageWikiLink RNA.
- Cas9 wikiPageWikiLink RNA_interference.
- Cas9 wikiPageWikiLink Repressor.
- Cas9 wikiPageWikiLink Stoichiometry.
- Cas9 wikiPageWikiLink Streptococcus_pyogenes.
- Cas9 wikiPageWikiLink Transcription_(genetics).
- Cas9 wikiPageWikiLink Transcription_activator-like_effector_nuclease.
- Cas9 wikiPageWikiLink Urea.
- Cas9 wikiPageWikiLink Virus.
- Cas9 wikiPageWikiLink Zinc_finger_nuclease.
- Cas9 wikiPageWikiLink File:Cas9_Anders_DNA_bound_structure.png.
- Cas9 wikiPageWikiLink File:Cas9_Apo_Structure.png.
- Cas9 wikiPageWikiLink File:The_Stages_of_CRISPR_immunity.svg.
- Cas9 wikiPageWikiLinkText "Cas9".
- Cas9 wikiPageWikiLinkText "dCas9".
- Cas9 altsymbols "SPy_1046".
- Cas9 caption "Crystal structure of S pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 A ˚ resolution.".
- Cas9 chromosome "genomic".
- Cas9 ecnumber "3.1".
- Cas9 entrezchromosome "NC_002737.2".
- Cas9 genlocEnd "859472".
- Cas9 genlocStart "854134".
- Cas9 organism "Streptococcus pyogenes".
- Cas9 refseqprotein "NP_269215.1".
- Cas9 symbol "cas9".
- Cas9 taxid "301447".
- Cas9 uniprot "Q99ZW2".
- Cas9 wikiPageUsesTemplate Template:Cite_journal.
- Cas9 wikiPageUsesTemplate Template:Infobox_nonhuman_protein.
- Cas9 wikiPageUsesTemplate Template:Reflist.
- Cas9 subject Category:Enzymes.
- Cas9 subject Category:Genetic_engineering.
- Cas9 subject Category:Repetitive_DNA_sequences.
- Cas9 hypernym Enzyme.
- Cas9 type Protein.
- Cas9 comment "Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorize and later interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA.".
- Cas9 label "Cas9".
- Cas9 sameAs Q16965677.
- Cas9 sameAs Cas9.
- Cas9 sameAs Cas9.
- Cas9 sameAs Cas9.
- Cas9 sameAs Cas9.
- Cas9 sameAs m.0_v3mxm.
- Cas9 sameAs Cas9.
- Cas9 sameAs Cas9.
- Cas9 sameAs Q16965677.
- Cas9 wasDerivedFrom Cas9?oldid=700778764.
- Cas9 depiction Crystal_Structure_of_Cas9_in_Complex_with_Guide_RNA_and_Target_DNA.jpg.
- Cas9 isPrimaryTopicOf Cas9.