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- Cas_Tools abstract "This page describes a list of software platforms and bioinformatics tools built to facilitate the design of guide RNAs (gRNAs) for use with the CRISPR/Cas system.The CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated nucleases) system was originally discovered to be an acquired immune response mechanism used by archaea and bacteria. It has since been adopted for use as a tool in the genetic engineering of higher organisms.Designing an appropriate gRNA is an important element of genome editing with the CRISPR/Cas system. A gRNA can and at times does have unintended interactions (\"off-targets\") with other locations of the genome of interest. For a given candidate gRNA, these tools report its list of potential off-targets in the genome thereby allowing the designer to evaluate its suitability prior to embarking on any experiments.Scientists have also begun exploring the mechanics of the CRISPR/Cas system and what governs how good, or active, a gRNA is at directing the Cas nuclease to a specific location of the genome of interest. As a result of this work, new methods of assessing a gRNA for its 'activity' have been published, and it is now best practice to consider both the unintended interactions of a gRNA as well as the predicted activity of a gRNA at the design stage.The below table lists available tools and their attributes, and includes links to the corresponding websites.".
- Cas_Tools wikiPageExternalLink crispr.mit.edu.
- Cas_Tools wikiPageExternalLink cas9.wicp.net.
- Cas_Tools wikiPageExternalLink crispr.cos.uni-heidelberg.de.
- Cas_Tools wikiPageExternalLink crispr.cos.uni-heidelberg.de.
- Cas_Tools wikiPageExternalLink crispr.mit.edu.
- Cas_Tools wikiPageExternalLink crispr-rna-configurator.
- Cas_Tools wikiPageExternalLink crispr-specificity-tool.
- Cas_Tools wikiPageExternalLink crispy.
- Cas_Tools wikiPageExternalLink doenchScore.py.
- Cas_Tools wikiPageExternalLink CRISPRseek.html.
- Cas_Tools wikiPageExternalLink sgrna-design.
- Cas_Tools wikiPageExternalLink cas-offinder.
- Cas_Tools wikiPageExternalLink portable.
- Cas_Tools wikiPageExternalLink ?utm_source=crispr-tools&utm_medium=nishant&utm_campaign=wiki.
- Cas_Tools wikiPageExternalLink crispr.
- Cas_Tools wikiPageExternalLink crispr.
- Cas_Tools wikiPageExternalLink chopchop.rc.fas.harvard.edu.
- Cas_Tools wikiPageExternalLink Off-Spotter.
- Cas_Tools wikiPageExternalLink Off-Spotter.
- Cas_Tools wikiPageExternalLink off-spotter-code.
- Cas_Tools wikiPageExternalLink remote.
- Cas_Tools wikiPageExternalLink www.deskgen.com.
- Cas_Tools wikiPageExternalLink input.
- Cas_Tools wikiPageExternalLink startCas9.
- Cas_Tools wikiPageID "45313845".
- Cas_Tools wikiPageLength "9268".
- Cas_Tools wikiPageOutDegree "23".
- Cas_Tools wikiPageRevisionID "705006836".
- Cas_Tools wikiPageWikiLink Archaea.
- Cas_Tools wikiPageWikiLink Bacteria.
- Cas_Tools wikiPageWikiLink Bioconductor.
- Cas_Tools wikiPageWikiLink Broad_Institute.
- Cas_Tools wikiPageWikiLink CRISPR.
- Cas_Tools wikiPageWikiLink Category:Genetic_engineering.
- Cas_Tools wikiPageWikiLink Coding_region.
- Cas_Tools wikiPageWikiLink DNA2.0.
- Cas_Tools wikiPageWikiLink Dharmacon,_Inc..
- Cas_Tools wikiPageWikiLink Exon.
- Cas_Tools wikiPageWikiLink Feng_Zhang.
- Cas_Tools wikiPageWikiLink Five_prime_untranslated_region.
- Cas_Tools wikiPageWikiLink Genetic_engineering.
- Cas_Tools wikiPageWikiLink Guide_RNA.
- Cas_Tools wikiPageWikiLink Harvard_University.
- Cas_Tools wikiPageWikiLink Heidelberg_University.
- Cas_Tools wikiPageWikiLink Long_non-coding_RNA.
- Cas_Tools wikiPageWikiLink Messenger_RNA.
- Cas_Tools wikiPageWikiLink Protospacer_adjacent_motif.
- Cas_Tools wikiPageWikiLink RNA_splicing.
- Cas_Tools wikiPageWikiLink Seoul_National_University.
- Cas_Tools wikiPageWikiLink Thomas_Jefferson_University.
- Cas_Tools wikiPageWikiLink Three_prime_untranslated_region.
- Cas_Tools wikiPageWikiLinkText "CRISPR/Cas Tools".
- Cas_Tools wikiPageWikiLinkText "gRNA designer".
- Cas_Tools wikiPageUsesTemplate Template:Reflist.
- Cas_Tools subject Category:Genetic_engineering.
- Cas_Tools comment "This page describes a list of software platforms and bioinformatics tools built to facilitate the design of guide RNAs (gRNAs) for use with the CRISPR/Cas system.The CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated nucleases) system was originally discovered to be an acquired immune response mechanism used by archaea and bacteria.".
- Cas_Tools label "CRISPR/Cas Tools".
- Cas_Tools sameAs m.012sp0nc.
- Cas_Tools wasDerivedFrom Cas_Tools?oldid=705006836.
- Cas_Tools isPrimaryTopicOf Cas_Tools.