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- CAG_promoter abstract "The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors.CAG promoter was constructed in the lab of Dr Jun-ichi Miyazaki from the following sequences: (C) the cytomegalovirus (CMV) early enhancer element, (A) the promoter, the first exon and the first intron of chicken beta-actin gene,(G) the splice acceptor of the rabbit beta-globin geneThe resulting synthetic element was used in the pCAGGS expression vector.Although the whole construct is commonly referred to as the \"CAG promoter\", it is not a promoter in a strict sense, as it includes a part of the transcribed sequence (two exons and an intron) and enhancer elements. In addition to the CMV immediate early enhancer, the intron of the chicken beta actin gene contains an enhancer element, which is highly conserved among vertebrates. The 3' part of the promoter has high GC content and is thus refractory to PCR amplification.".
- CAG_promoter wikiPageID "26827604".
- CAG_promoter wikiPageLength "2328".
- CAG_promoter wikiPageOutDegree "5".
- CAG_promoter wikiPageRevisionID "631044766".
- CAG_promoter wikiPageWikiLink Beta-actin.
- CAG_promoter wikiPageWikiLink Category:Gene_expression.
- CAG_promoter wikiPageWikiLink Cytomegalovirus.
- CAG_promoter wikiPageWikiLink Enhancer_(genetics).
- CAG_promoter wikiPageWikiLink Expression_vector.
- CAG_promoter wikiPageWikiLinkText "CAG promoter".
- CAG_promoter wikiPageUsesTemplate Template:Molecular-cell-biology-stub.
- CAG_promoter wikiPageUsesTemplate Template:Orphan.
- CAG_promoter wikiPageUsesTemplate Template:Reflist.
- CAG_promoter subject Category:Gene_expression.
- CAG_promoter hypernym Promoter.
- CAG_promoter type Person.
- CAG_promoter type Process.
- CAG_promoter comment "The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors.CAG promoter was constructed in the lab of Dr Jun-ichi Miyazaki from the following sequences: (C) the cytomegalovirus (CMV) early enhancer element, (A) the promoter, the first exon and the first intron of chicken beta-actin gene,(G) the splice acceptor of the rabbit beta-globin geneThe resulting synthetic element was used in the pCAGGS expression vector.Although the whole construct is commonly referred to as the \"CAG promoter\", it is not a promoter in a strict sense, as it includes a part of the transcribed sequence (two exons and an intron) and enhancer elements. ".
- CAG_promoter label "CAG promoter".
- CAG_promoter sameAs Q5008599.
- CAG_promoter sameAs m.0bm9lb3.
- CAG_promoter sameAs Q5008599.
- CAG_promoter wasDerivedFrom CAG_promoter?oldid=631044766.
- CAG_promoter isPrimaryTopicOf CAG_promoter.