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- BglII abstract "BglII (pronounced \"begel two\") is a type II restriction endonuclease enzyme isolated from certain strains of Bacillus globigii.The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition.Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix. Each monomer is 223 amino acids and symmetrically bind both sides of the unique palindromic nucleotide sequence AGATCT, cleaving the scissile phosphodiester bond between the first Adenine and Guanine nucleotides on both strands of the DNA molecule, creating sticky ends with 5' end overhangs.Being a type II restriction enzyme, BglII does not require ATP (adenosine triphosphate) for its enzymatic function, but only requires association with a divalent metal cation, most likely Mg2+. Unlike other restriction enzymes of its class, BglII has been show to possess some unique structural characteristics, such as a β-sandwich subdomain, and appears to undergo a unique conformational change upon dimerization, but its overall structure and mechanism of catalysis remain consistent with other type II restriction enzymes.Restriction Endonuclease enzymes play a very important role in modern molecular cloning techniques. Because of their unique recognition/cut sites, restriction enzymes can be used to precisely cut DNA in specific locations in a controllable manner. Once cut, scientist can then insert the desired DNA fragment possessing \"sticky ends\" into a circular DNA molecule, and ligate them together to create an engineered cloning vector.".
- BglII symbol "Endonuc-BglII".
- BglII thumbnail BglII_cut_sites.JPG?width=300.
- BglII wikiPageExternalLink rebase.html.
- BglII wikiPageExternalLink index.html.
- BglII wikiPageExternalLink result_flat.php4?ecno=3.1.21.4.
- BglII wikiPageExternalLink mmdbsrv.cgi?uid=48647.
- BglII wikiPageExternalLink viewer.fcgi?db=protein&id=20178143.
- BglII wikiPageExternalLink entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6173168&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum.
- BglII wikiPageExternalLink entrez?db=protein&cmd=search&term=Q45488.
- BglII wikiPageExternalLink explore.do?structureId=1DFM.
- BglII wikiPageID "12678511".
- BglII wikiPageLength "9578".
- BglII wikiPageOutDegree "30".
- BglII wikiPageRevisionID "708129463".
- BglII wikiPageWikiLink Adenosine_triphosphate.
- BglII wikiPageWikiLink Bacillus_atrophaeus.
- BglII wikiPageWikiLink BamHI.
- BglII wikiPageWikiLink Beta_sheet.
- BglII wikiPageWikiLink Category:Bacterial_enzymes.
- BglII wikiPageWikiLink Category:Molecular_biology.
- BglII wikiPageWikiLink Category:Restriction_enzymes.
- BglII wikiPageWikiLink Cloning_vector.
- BglII wikiPageWikiLink Conformational_change.
- BglII wikiPageWikiLink DNA.
- BglII wikiPageWikiLink Divalent.
- BglII wikiPageWikiLink EcoRI.
- BglII wikiPageWikiLink Endonuclease.
- BglII wikiPageWikiLink Enzyme.
- BglII wikiPageWikiLink File:BglII_cut_sites.JPG.
- BglII wikiPageWikiLink FokI.
- BglII wikiPageWikiLink Genetic_recombination.
- BglII wikiPageWikiLink Hydroxide.
- BglII wikiPageWikiLink Molecular_cloning.
- BglII wikiPageWikiLink Nucleic_acid_double_helix.
- BglII wikiPageWikiLink Nucleophile.
- BglII wikiPageWikiLink Palindrome.
- BglII wikiPageWikiLink Phosphodiester_bond.
- BglII wikiPageWikiLink Protein_dimer.
- BglII wikiPageWikiLink Transition_state.
- BglII wikiPageWikiLink Transposable_element.
- BglII wikiPageWikiLink Α-helicies.
- BglII wikiPageWikiLink File:BglIIactivesite.png.
- BglII wikiPageWikiLink File:BglIIboundactivesite.png.
- BglII wikiPageWikiLink File:Proposedmechanismforbglii.gif.
- BglII wikiPageWikiLinkText "''Bgl''II".
- BglII wikiPageWikiLinkText "BglII".
- BglII caption "structure of restriction endonuclease BstYI bound to non-cognate DNA".
- BglII interpro "IPR015278".
- BglII name "Restriction endonuclease BglII".
- BglII pfam "PF09195".
- BglII pfamClan "CL0236".
- BglII scop "1".
- BglII symbol "Endonuc-BglII".
- BglII wikiPageUsesTemplate Template:Infobox_protein_family.
- BglII wikiPageUsesTemplate Template:Reflist.
- BglII wikiPageUsesTemplate Template:Technical.
- BglII subject Category:Bacterial_enzymes.
- BglII subject Category:Molecular_biology.
- BglII subject Category:Restriction_enzymes.
- BglII hypernym Enzyme.
- BglII type Biomolecule.
- BglII type Protein.
- BglII type Chemical.
- BglII type Thing.
- BglII type Q206229.
- BglII type Q8054.
- BglII comment "BglII (pronounced \"begel two\") is a type II restriction endonuclease enzyme isolated from certain strains of Bacillus globigii.The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition.Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix.".
- BglII label "BglII".
- BglII sameAs Q4900470.
- BglII sameAs BglII.
- BglII sameAs BglII.
- BglII sameAs m.02x05w7.
- BglII sameAs BglII.
- BglII sameAs Q4900470.
- BglII wasDerivedFrom BglII?oldid=708129463.
- BglII depiction BglII_cut_sites.JPG.
- BglII isPrimaryTopicOf BglII.