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- Alanine_scanning abstract "In molecular biology, alanine scanning is a technique used to determine the contribution of a specific residue to the stability or function of given protein. Alanine is used because of its non-bulky, chemically inert, methyl functional group that nevertheless mimics the secondary structure preferences that many of the other amino acids possess. Sometimes bulky amino acids such as valine or leucine are used in cases where conservation of the size of mutated residues is needed. This technique can also be used to determine whether the side chain of a specific residue plays a significant role in bioactivity. This is usually accomplished by site-directed mutagenesis or randomly by creating a PCR library. Furthermore, computational methods to estimate thermodynamic parameters based on a theoretical alanine substitutions have been developed.This technique is rapid, because many side chains are analyzed simultaneously and the need for protein purification and biophysical analysis is circumvented. The technology is very mature at this point and is widely used in biochemical fields. The data can be tested by IR, NMR Spectroscopy, mathematical methods, bioassays, etc.One good example of alanine scanning is the examination of the role of charged residues on the surface of proteins. In a systematic study on the roles of conserved charged residues on the surface of epithelial sodium channel (ENaC), alanine scanning was used to reveal the importance of charged residues for the process of transport of the proteins to the cell surface.".
- Alanine_scanning thumbnail Alanine_Scan.png?width=300.
- Alanine_scanning wikiPageID "24766064".
- Alanine_scanning wikiPageLength "6214".
- Alanine_scanning wikiPageOutDegree "22".
- Alanine_scanning wikiPageRevisionID "605820499".
- Alanine_scanning wikiPageWikiLink Alanine.
- Alanine_scanning wikiPageWikiLink Amino_acid.
- Alanine_scanning wikiPageWikiLink Bacillus_thuringiensis.
- Alanine_scanning wikiPageWikiLink Biological_activity.
- Alanine_scanning wikiPageWikiLink Biomolecular_structure.
- Alanine_scanning wikiPageWikiLink Category:Molecular_biology_techniques.
- Alanine_scanning wikiPageWikiLink Culex_pipiens.
- Alanine_scanning wikiPageWikiLink Cyclotide.
- Alanine_scanning wikiPageWikiLink Epithelial_sodium_channel.
- Alanine_scanning wikiPageWikiLink High-throughput_screening.
- Alanine_scanning wikiPageWikiLink Infrared.
- Alanine_scanning wikiPageWikiLink Leucine.
- Alanine_scanning wikiPageWikiLink Molecular_biology.
- Alanine_scanning wikiPageWikiLink Mutagenesis_(molecular_biology_technique).
- Alanine_scanning wikiPageWikiLink Nuclear_magnetic_resonance_spectroscopy.
- Alanine_scanning wikiPageWikiLink Polymerase_chain_reaction.
- Alanine_scanning wikiPageWikiLink Proteinogenic_amino_acid.
- Alanine_scanning wikiPageWikiLink Site-directed_mutagenesis.
- Alanine_scanning wikiPageWikiLink Valine.
- Alanine_scanning wikiPageWikiLink File:Alanine_Scan.png.
- Alanine_scanning wikiPageWikiLinkText "Alanine scanning".
- Alanine_scanning wikiPageWikiLinkText "alanine scanning".
- Alanine_scanning wikiPageUsesTemplate Template:Cite_book.
- Alanine_scanning wikiPageUsesTemplate Template:Refbegin.
- Alanine_scanning wikiPageUsesTemplate Template:Refend.
- Alanine_scanning wikiPageUsesTemplate Template:Reflist.
- Alanine_scanning subject Category:Molecular_biology_techniques.
- Alanine_scanning hypernym Technique.
- Alanine_scanning type TopicalConcept.
- Alanine_scanning type Technique.
- Alanine_scanning comment "In molecular biology, alanine scanning is a technique used to determine the contribution of a specific residue to the stability or function of given protein. Alanine is used because of its non-bulky, chemically inert, methyl functional group that nevertheless mimics the secondary structure preferences that many of the other amino acids possess. Sometimes bulky amino acids such as valine or leucine are used in cases where conservation of the size of mutated residues is needed.".
- Alanine_scanning label "Alanine scanning".
- Alanine_scanning sameAs Q4708243.
- Alanine_scanning sameAs m.080q8tt.
- Alanine_scanning sameAs Q4708243.
- Alanine_scanning wasDerivedFrom Alanine_scanning?oldid=605820499.
- Alanine_scanning depiction Alanine_Scan.png.
- Alanine_scanning isPrimaryTopicOf Alanine_scanning.