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- ABTS abstract "In biochemistry, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone with blue multicopper oxidase enzymes (such as laccase or bilirubin oxidase). Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide-producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample. The formal reduction potentials for ABTS are high enough for it to act as an electron donor for the reduction of oxo species such as molecular oxygen and hydrogen peroxide, particularly at the less-extreme pH values encountered in biological catalysis. Under these conditions, the sulfonate groups are fully deprotonated and the mediator exists as a dianion..ABTS–· + e– → ABTS2– E°′ = 0.67 V vs SHEABTS + e– → ABTS–· E°′ = 1.08 V vs SHEThis compound is chosen because the enzyme facilitates the reaction with hydrogen peroxide, turning it into a green and soluble end-product. Its new absorbance maximum of 420 nm light (ε = 3.6 × 104 M–1 cm–1) can easily be followed with a spectrophotometer, a common laboratory instrument. It is sometimes used as part of a glucose estimating reagent when finding glucose concentrations of solutions such as blood serum.ABTS is also frequently used by the food industry and agricultural researchers to measure the antioxidant capacities of foods. In this assay, ABTS is converted to its radical cation by addition of sodium persulfate. This radical cation is blue in color and absorbs light at 734 nm. The ABTS radical cation is reactive towards most antioxidants including phenolics, thiols and Vitamin C. During this reaction, the blue ABTS radical cation is converted back to its colorless neutral form. The reaction may be monitored spectrophotometrically. This assay is often referred to as the Trolox equivalent antioxidant capacity (TEAC) assay. The reactivity of the various antioxidants tested are compared to that of Trolox, which is a water-soluble analog of vitamin E.".
- ABTS iupacName "2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)".
- ABTS thumbnail ABTS_structure.png?width=300.
- ABTS wikiPageID "2730088".
- ABTS wikiPageLength "8336".
- ABTS wikiPageOutDegree "36".
- ABTS wikiPageRevisionID "695718199".
- ABTS wikiPageWikiLink Absorbance.
- ABTS wikiPageWikiLink Antioxidant.
- ABTS wikiPageWikiLink Bilirubin_oxidase.
- ABTS wikiPageWikiLink Biochemistry.
- ABTS wikiPageWikiLink Category:Biochemistry_methods.
- ABTS wikiPageWikiLink Category:Enzyme_kinetics.
- ABTS wikiPageWikiLink Category:Sulfonic_acids.
- ABTS wikiPageWikiLink ELISA.
- ABTS wikiPageWikiLink Enzyme.
- ABTS wikiPageWikiLink Enzyme_kinetics.
- ABTS wikiPageWikiLink Glucose.
- ABTS wikiPageWikiLink Horseradish_peroxidase.
- ABTS wikiPageWikiLink Hydrogen_peroxide.
- ABTS wikiPageWikiLink Laccase.
- ABTS wikiPageWikiLink Light.
- ABTS wikiPageWikiLink Multicopper_oxidase.
- ABTS wikiPageWikiLink Peroxidase.
- ABTS wikiPageWikiLink Polyphenol.
- ABTS wikiPageWikiLink Reagent.
- ABTS wikiPageWikiLink Reduction_potential.
- ABTS wikiPageWikiLink Serum_(blood).
- ABTS wikiPageWikiLink Sodium_persulfate.
- ABTS wikiPageWikiLink Solubility.
- ABTS wikiPageWikiLink Spectrophotometry.
- ABTS wikiPageWikiLink Standard_hydrogen_electrode.
- ABTS wikiPageWikiLink Substrate_(chemistry).
- ABTS wikiPageWikiLink Thiol.
- ABTS wikiPageWikiLink Trolox.
- ABTS wikiPageWikiLink Trolox_equivalent_antioxidant_capacity.
- ABTS wikiPageWikiLink Vitamin_C.
- ABTS wikiPageWikiLink Vitamin_E.
- ABTS wikiPageWikiLinkText "ABTS".
- ABTS imagefile "ABTS structure.png".
- ABTS iupacname "22".
- ABTS verifiedfields "changed".
- ABTS verifiedrevid "477235168".
- ABTS watchedfields "changed".
- ABTS wikiPageUsesTemplate Template:Cascite.
- ABTS wikiPageUsesTemplate Template:Chembox.
- ABTS wikiPageUsesTemplate Template:Chembox_Hazards.
- ABTS wikiPageUsesTemplate Template:Chembox_Identifiers.
- ABTS wikiPageUsesTemplate Template:Chembox_Properties.
- ABTS wikiPageUsesTemplate Template:Chemspidercite.
- ABTS wikiPageUsesTemplate Template:R36.
- ABTS wikiPageUsesTemplate Template:R37.
- ABTS wikiPageUsesTemplate Template:R38.
- ABTS wikiPageUsesTemplate Template:Reflist.
- ABTS wikiPageUsesTemplate Template:S26.
- ABTS wikiPageUsesTemplate Template:S36.
- ABTS wikiPageUsesTemplate Template:Stdinchicite.
- ABTS subject Category:Biochemistry_methods.
- ABTS subject Category:Enzyme_kinetics.
- ABTS subject Category:Sulfonic_acids.
- ABTS hypernym Compound.
- ABTS type ChemicalCompound.
- ABTS type ChemicalSubstance.
- ABTS type Group.
- ABTS type Group.
- ABTS type Method.
- ABTS type Technique.
- ABTS type ChemicalObject.
- ABTS type Thing.
- ABTS type Q11173.
- ABTS comment "In biochemistry, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone with blue multicopper oxidase enzymes (such as laccase or bilirubin oxidase).".
- ABTS label "ABTS".
- ABTS sameAs Q287582.
- ABTS sameAs ABTS.
- ABTS sameAs ایبیتیاس.
- ABTS sameAs ABTS.
- ABTS sameAs m.07_hrx.
- ABTS sameAs ABTS.
- ABTS sameAs ABTS.
- ABTS sameAs ABTS.
- ABTS sameAs Q287582.
- ABTS wasDerivedFrom ABTS?oldid=695718199.
- ABTS depiction ABTS_structure.png.
- ABTS isPrimaryTopicOf ABTS.