Matches in DBpedia 2015-10 for { <http://dbpedia.org/resource/Thermal_shift_assay> ?p ?o }
Showing triples 1 to 79 of
79
with 100 triples per page.
- Thermal_shift_assay abstract "A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.".
- Thermal_shift_assay thumbnail Thermal_Shift_Assay.png?width=300.
- Thermal_shift_assay wikiPageExternalLink SYPRO_Orange.
- Thermal_shift_assay wikiPageExternalLink Stargazer-2.
- Thermal_shift_assay wikiPageExternalLink protein.html.
- Thermal_shift_assay wikiPageExternalLink prometheus-series.
- Thermal_shift_assay wikiPageExternalLink www.unchainedlabs.com.
- Thermal_shift_assay wikiPageExternalLink 24570.
- Thermal_shift_assay wikiPageID "38876059".
- Thermal_shift_assay wikiPageLength "26309".
- Thermal_shift_assay wikiPageOutDegree "46".
- Thermal_shift_assay wikiPageRevisionID "681633824".
- Thermal_shift_assay wikiPageWikiLink 8-Anilinonaphthalene-1-sulfonic_acid.
- Thermal_shift_assay wikiPageWikiLink Assay.
- Thermal_shift_assay wikiPageWikiLink Biochemical_assays.
- Thermal_shift_assay wikiPageWikiLink Biomolecular_structure.
- Thermal_shift_assay wikiPageWikiLink Category:Microbiology_techniques.
- Thermal_shift_assay wikiPageWikiLink Centrifugation.
- Thermal_shift_assay wikiPageWikiLink Circular_dichroism.
- Thermal_shift_assay wikiPageWikiLink Cofactor_(biochemistry).
- Thermal_shift_assay wikiPageWikiLink Counterion.
- Thermal_shift_assay wikiPageWikiLink Cysteine.
- Thermal_shift_assay wikiPageWikiLink Differential_scanning_calorimetry.
- Thermal_shift_assay wikiPageWikiLink Dispersity.
- Thermal_shift_assay wikiPageWikiLink Drug_discovery.
- Thermal_shift_assay wikiPageWikiLink Evaporation.
- Thermal_shift_assay wikiPageWikiLink FAAH.
- Thermal_shift_assay wikiPageWikiLink Fast_parallel_proteolysis.
- Thermal_shift_assay wikiPageWikiLink Fast_protein_liquid_chromatography.
- Thermal_shift_assay wikiPageWikiLink Fatty_acid_amide_hydrolase.
- Thermal_shift_assay wikiPageWikiLink Fluorescence_spectroscopy.
- Thermal_shift_assay wikiPageWikiLink Fluorescent_tag.
- Thermal_shift_assay wikiPageWikiLink Fluorometer.
- Thermal_shift_assay wikiPageWikiLink GPCR.
- Thermal_shift_assay wikiPageWikiLink G_protein–coupled_receptor.
- Thermal_shift_assay wikiPageWikiLink Glutathione_S-transferase.
- Thermal_shift_assay wikiPageWikiLink Green_fluorescent_protein.
- Thermal_shift_assay wikiPageWikiLink His-tag.
- Thermal_shift_assay wikiPageWikiLink Intrinsically_disordered_proteins.
- Thermal_shift_assay wikiPageWikiLink Mannan-binding_lectin.
- Thermal_shift_assay wikiPageWikiLink Mannose-binding_protein.
- Thermal_shift_assay wikiPageWikiLink Molecular_mass.
- Thermal_shift_assay wikiPageWikiLink Molecular_weight.
- Thermal_shift_assay wikiPageWikiLink Monodispersity.
- Thermal_shift_assay wikiPageWikiLink NanoDSF.
- Thermal_shift_assay wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Thermal_shift_assay wikiPageWikiLink Polyhistidine-tag.
- Thermal_shift_assay wikiPageWikiLink Protein_engineering.
- Thermal_shift_assay wikiPageWikiLink QPCR.
- Thermal_shift_assay wikiPageWikiLink Ramp.
- Thermal_shift_assay wikiPageWikiLink Real-time_polymerase_chain_reaction.
- Thermal_shift_assay wikiPageWikiLink SDS-PAGE.
- Thermal_shift_assay wikiPageWikiLink Silicone_oil.
- Thermal_shift_assay wikiPageWikiLink Size-exclusion_chromatography.
- Thermal_shift_assay wikiPageWikiLink Size_exclusion_chromatography.
- Thermal_shift_assay wikiPageWikiLink Static_light_scattering.
- Thermal_shift_assay wikiPageWikiLink Tertiary_structure.
- Thermal_shift_assay wikiPageWikiLink Thermolysin.
- Thermal_shift_assay wikiPageWikiLink Transmembrane_protein.
- Thermal_shift_assay wikiPageWikiLink Transmembrane_proteins.
- Thermal_shift_assay wikiPageWikiLink Western_blot.
- Thermal_shift_assay wikiPageWikiLink X-ray_crystal_structure.
- Thermal_shift_assay wikiPageWikiLink X-ray_crystallography.
- Thermal_shift_assay wikiPageWikiLink File:Thermal_Shift_Assay.png.
- Thermal_shift_assay wikiPageWikiLinkText "Differential scanning Fluorimetry".
- Thermal_shift_assay wikiPageWikiLinkText "Thermal shift assay".
- Thermal_shift_assay wikiPageWikiLinkText "thermal shift assay".
- Thermal_shift_assay hasPhotoCollection Thermal_shift_assay.
- Thermal_shift_assay wikiPageUsesTemplate Template:Reflist.
- Thermal_shift_assay subject Category:Microbiology_techniques.
- Thermal_shift_assay comment "A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor.".
- Thermal_shift_assay label "Thermal shift assay".
- Thermal_shift_assay sameAs Thermal_Shift_Assay.
- Thermal_shift_assay sameAs m.0rysp4b.
- Thermal_shift_assay sameAs Q17098679.
- Thermal_shift_assay sameAs Q17098679.
- Thermal_shift_assay wasDerivedFrom Thermal_shift_assay?oldid=681633824.
- Thermal_shift_assay depiction Thermal_Shift_Assay.png.
- Thermal_shift_assay isPrimaryTopicOf Thermal_shift_assay.