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- Synchronous_culture abstract "A synchronous or synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage.Since numerous factors influence the cell cycle, some of them stochastic (random), normal, non-synchronous cultures have cells in all stages of the cell cycle. Obtaining a culture with a unified cell-cycle stage is very useful for biological research. Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be easily estimated, and quantitative experimental results can simply be divided in the number of cells to obtain values that apply to a single cell. Synchronous cultures have been extensively used to address questions regarding cell cycle and growth, and the effects of various factors on these.Synchronous cultures can be obtained in several ways: External conditions can be changed, so as to arrest growth of all cells in the culture, and then changed again to resume growth. The newly growing cells are now all starting to grow at the same stage, and they are synchronized. For example, for photosynthetic cells light can be eliminated for several hours and then re-introduced. Another method is to eliminate an essential nutrient from the growth medium and later to re-introduce it. Cell growth can also be arrested using chemical growth inhibitors. After growth has completely stopped for all cells, the inhibitor can be easily removed from the culture and the cells then begin to grow synchronously. Nocodazole, for example, is often used in biological research for this purpose. Cells in different growth stages have different physical properties. Cells in a culture can thus be physically separated based on their density or size, for instance. This can be achieved using centrifugation (for density) or filtration (for size). In the Helmstetter-Cummings technique, a bacterial culture is filtered through a membrane. Most bacteria pass through, but some remain bound to the membrane. Fresh medium is then applied to the membrane and the bound bacteria start to grow. Newborn bacteria that detach from the membrane are now all at the same stage of growth; they are collected in a flask that now harbors a synchronous culture.".
- Synchronous_culture wikiPageExternalLink synch.htm.
- Synchronous_culture wikiPageID "26446678".
- Synchronous_culture wikiPageLength "2744".
- Synchronous_culture wikiPageOutDegree "19".
- Synchronous_culture wikiPageRevisionID "632603470".
- Synchronous_culture wikiPageWikiLink Category:Cell_culture.
- Synchronous_culture wikiPageWikiLink Category:Microbiology_techniques.
- Synchronous_culture wikiPageWikiLink Category:Molecular_biology_techniques.
- Synchronous_culture wikiPageWikiLink Cell_(biology).
- Synchronous_culture wikiPageWikiLink Cell_culture.
- Synchronous_culture wikiPageWikiLink Cell_cycle.
- Synchronous_culture wikiPageWikiLink Cell_growth.
- Synchronous_culture wikiPageWikiLink Centrifugation.
- Synchronous_culture wikiPageWikiLink Density.
- Synchronous_culture wikiPageWikiLink Filtration.
- Synchronous_culture wikiPageWikiLink Growth_inhibition.
- Synchronous_culture wikiPageWikiLink Growth_medium.
- Synchronous_culture wikiPageWikiLink Light.
- Synchronous_culture wikiPageWikiLink Microbiological_culture.
- Synchronous_culture wikiPageWikiLink Nocodazole.
- Synchronous_culture wikiPageWikiLink Nutrient.
- Synchronous_culture wikiPageWikiLink Photosynthesis.
- Synchronous_culture wikiPageWikiLink Random.
- Synchronous_culture wikiPageWikiLink Randomness.
- Synchronous_culture wikiPageWikiLink Stochastic.
- Synchronous_culture wikiPageWikiLinkText "Synchronous culture".
- Synchronous_culture wikiPageWikiLinkText "synchronous culture".
- Synchronous_culture hasPhotoCollection Synchronous_culture.
- Synchronous_culture wikiPageUsesTemplate Template:Multiple_issues.
- Synchronous_culture subject Category:Cell_culture.
- Synchronous_culture subject Category:Microbiology_techniques.
- Synchronous_culture subject Category:Molecular_biology_techniques.
- Synchronous_culture hypernym Culture.
- Synchronous_culture type Article.
- Synchronous_culture type Magazine.
- Synchronous_culture type Article.
- Synchronous_culture type Organism.
- Synchronous_culture type Technique.
- Synchronous_culture type Organism.
- Synchronous_culture comment "A synchronous or synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage.Since numerous factors influence the cell cycle, some of them stochastic (random), normal, non-synchronous cultures have cells in all stages of the cell cycle. Obtaining a culture with a unified cell-cycle stage is very useful for biological research.".
- Synchronous_culture label "Synchronous culture".
- Synchronous_culture sameAs m.0bbwlvp.
- Synchronous_culture sameAs Q7662213.
- Synchronous_culture sameAs Q7662213.
- Synchronous_culture wasDerivedFrom Synchronous_culture?oldid=632603470.
- Synchronous_culture isPrimaryTopicOf Synchronous_culture.