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- Synapto-pHluorin abstract "Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release. It consists of a pH-sensitive form of green fluorescent protein (GFP) fused to the luminal side of a vesicle-associated membrane protein (VAMP). At the acidic pH inside transmitter vesicles, synapto-pHluorin is non-fluorescent. When vesicles get released, synapto-pHluorin is exposed to the neutral extracellular space and the presynaptic terminal becomes brightly fluorescent. Following endocytosis, vesicles become re-acidified and the cycle can start again. Chemical alkalinization of all vesicles is often used for normalization of the synapto-pHluorin signals. In the following video a typical Synapto-pHluorin experiment is shown (VGluT1-pHluorin in this case).REDIRECT [1]".
- Synapto-pHluorin wikiPageExternalLink watch?v=RrJE4gNI_ec.
- Synapto-pHluorin wikiPageID "21377466".
- Synapto-pHluorin wikiPageLength "1967".
- Synapto-pHluorin wikiPageOutDegree "13".
- Synapto-pHluorin wikiPageRevisionID "656783729".
- Synapto-pHluorin wikiPageWikiLink Alkalinity.
- Synapto-pHluorin wikiPageWikiLink Alkalinization.
- Synapto-pHluorin wikiPageWikiLink Beta_cell.
- Synapto-pHluorin wikiPageWikiLink Beta_cells.
- Synapto-pHluorin wikiPageWikiLink Category:Cell_imaging.
- Synapto-pHluorin wikiPageWikiLink Category:Fluorescent_dyes.
- Synapto-pHluorin wikiPageWikiLink Category:Protein_methods.
- Synapto-pHluorin wikiPageWikiLink Category:Recombinant_proteins.
- Synapto-pHluorin wikiPageWikiLink Endocytosis.
- Synapto-pHluorin wikiPageWikiLink Gero_Miesenböck.
- Synapto-pHluorin wikiPageWikiLink Green_fluorescent_protein.
- Synapto-pHluorin wikiPageWikiLink Insulin.
- Synapto-pHluorin wikiPageWikiLink Neuroscience.
- Synapto-pHluorin wikiPageWikiLink Pancreas.
- Synapto-pHluorin wikiPageWikiLink Vesicle-associated_membrane_protein.
- Synapto-pHluorin wikiPageWikiLinkText "Synapto-pHluorin".
- Synapto-pHluorin wikiPageWikiLinkText "synapto-pHluorin".
- Synapto-pHluorin hasPhotoCollection Synapto-pHluorin.
- Synapto-pHluorin wikiPageUsesTemplate Template:Reflist.
- Synapto-pHluorin subject Category:Cell_imaging.
- Synapto-pHluorin subject Category:Fluorescent_dyes.
- Synapto-pHluorin subject Category:Protein_methods.
- Synapto-pHluorin subject Category:Recombinant_proteins.
- Synapto-pHluorin type Method.
- Synapto-pHluorin type Product.
- Synapto-pHluorin type Technique.
- Synapto-pHluorin comment "Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release. It consists of a pH-sensitive form of green fluorescent protein (GFP) fused to the luminal side of a vesicle-associated membrane protein (VAMP). At the acidic pH inside transmitter vesicles, synapto-pHluorin is non-fluorescent.".
- Synapto-pHluorin label "Synapto-pHluorin".
- Synapto-pHluorin sameAs m.05f4ssb.
- Synapto-pHluorin sameAs Q7662044.
- Synapto-pHluorin sameAs Q7662044.
- Synapto-pHluorin wasDerivedFrom Synapto-pHluorin?oldid=656783729.
- Synapto-pHluorin isPrimaryTopicOf Synapto-pHluorin.