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- Supravital_staining abstract "Supravital staining is a method of staining used in microscopy to examine living cells that have been removed from an organism. It differs from intravital staining, which is done by injecting or otherwise introducing the stain into the body. Thus a supravital stain may have a greater toxicity, as only a few cells need to survive it a short while. The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive and unfixed, outside the body, supravital stains are temporary in nature.The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes it possible to see the reticulofilamentous pattern of ribosomes characteristically precipitated in these live immature red blood cells by the supravital stains. By counting the number of such cells the rate of red blood cell formation can be determined, providing an insight into bone marrow activity and anemia. In contrast to supravital staining, when dyes like trypan blue or propidium iodide, which are either too bulky or too charged to cross the cell membrane (or are actively rapidly pumped out by live cells), are used to count the number of living cells ("negatively" stained) in a suspension; in this case the dye employed is one that is excluded from the living cells so that only dead cells are stained positively and this staining method is called vital staining.Supravital staining can be combined with cell surface antibody staining (immunofluorescence) for applications such as FACS analysis. Immunofluorescence can also be done within the interior of live cells by reversible cell permeabilization using the detergent Triton X-100. Adjusted carefully to the appropriate concentration for the number of cells, the pretreatment can permit access of molecules between 1 and 150 kilodaltons to the interior of the cell. Although antibodies may be used in a similar way in this context, the term "supravital stain" is typically reserved for smaller chemicals which possess suitable properties intrinsically.".
- Supravital_staining thumbnail Reticulocytes_Human_Blood_Supravital_Stain.jpg?width=300.
- Supravital_staining wikiPageID "35215338".
- Supravital_staining wikiPageLength "4303".
- Supravital_staining wikiPageOutDegree "29".
- Supravital_staining wikiPageRevisionID "603923417".
- Supravital_staining wikiPageWikiLink Anemia.
- Supravital_staining wikiPageWikiLink Antibody.
- Supravital_staining wikiPageWikiLink Atomic_mass_unit.
- Supravital_staining wikiPageWikiLink Bone_marrow.
- Supravital_staining wikiPageWikiLink Brilliant_cresyl_blue.
- Supravital_staining wikiPageWikiLink Category:Staining.
- Supravital_staining wikiPageWikiLink Cell_permeabilization.
- Supravital_staining wikiPageWikiLink Crystal_violet.
- Supravital_staining wikiPageWikiLink Flow_cytometry.
- Supravital_staining wikiPageWikiLink Fluorescence-activated_cell_sorting.
- Supravital_staining wikiPageWikiLink Hoechst_stain.
- Supravital_staining wikiPageWikiLink Immunofluorescence.
- Supravital_staining wikiPageWikiLink Intravital_staining.
- Supravital_staining wikiPageWikiLink Kilodalton.
- Supravital_staining wikiPageWikiLink Methyl_violet.
- Supravital_staining wikiPageWikiLink Microscopy.
- Supravital_staining wikiPageWikiLink New_methylene_blue.
- Supravital_staining wikiPageWikiLink Nile_blue.
- Supravital_staining wikiPageWikiLink Propidium_iodide.
- Supravital_staining wikiPageWikiLink Red_blood_cell.
- Supravital_staining wikiPageWikiLink Reticulocyte.
- Supravital_staining wikiPageWikiLink Ribosome.
- Supravital_staining wikiPageWikiLink Staining.
- Supravital_staining wikiPageWikiLink Toxicity.
- Supravital_staining wikiPageWikiLink Triton_X-100.
- Supravital_staining wikiPageWikiLink Trypan_blue.
- Supravital_staining wikiPageWikiLink Vital_stain.
- Supravital_staining wikiPageWikiLink File:Reticulocytes_Human_Blood_Supravital_Stain.jpg.
- Supravital_staining wikiPageWikiLinkText "Supravital staining".
- Supravital_staining wikiPageWikiLinkText "supravital".
- Supravital_staining hasPhotoCollection Supravital_staining.
- Supravital_staining subject Category:Staining.
- Supravital_staining hypernym Method.
- Supravital_staining type Software.
- Supravital_staining type Polymer.
- Supravital_staining comment "Supravital staining is a method of staining used in microscopy to examine living cells that have been removed from an organism. It differs from intravital staining, which is done by injecting or otherwise introducing the stain into the body. Thus a supravital stain may have a greater toxicity, as only a few cells need to survive it a short while.".
- Supravital_staining label "Supravital staining".
- Supravital_staining sameAs m.0j7hqgg.
- Supravital_staining sameAs Q7644766.
- Supravital_staining sameAs Q7644766.
- Supravital_staining wasDerivedFrom Supravital_staining?oldid=603923417.
- Supravital_staining depiction Reticulocytes_Human_Blood_Supravital_Stain.jpg.
- Supravital_staining isPrimaryTopicOf Supravital_staining.