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- Stopped_flow abstract "A stopped flow instrument is a rapid mixing device used to study the chemical kinetics of fast reactions in solution. In stopped-flow technique, the solutions are forced from syringes into a mixing chamber. After a very short period of flow, perhaps a few ms, the flow is stopped suddenly when the observation cell is filled by an opposing piston that is linked to a sensing switch that triggers the measuring device. Small volumes of solutions are used, and the kinetic equations for modeling the reactions are equivalent to those used in conventional methods in which concentration and time are measured. Stopped-flow is useful for studying fast reactions that have half-lives as short as a few milliseconds.After two or more solutions containing the reagents are mixed, they are studied by whatever experimental methods are deemed suitable. Different forms of spectroscopy and scattering of radiation are common methods used. The dead time is the time between the end of mixing the two solutions and the beginning of observation of the kinetics of the reaction. The usual dead time of a stopped flow apparatus is 1-2 milliseconds but some new devices have been developed that have dead times of 0.3-0.6 ms.A stopped flow instrument coupled to either a circular dichroism spectrometer or a fluorescence spectrometer is often used in the field of protein folding, to observe rapid unfolding and/or refolding of proteins. In a quenched flow instrument, the reaction is stopped after a certain amount of time has passed after mixing. The stopping of the reaction is called quenching and it can be achieved by various means, for example by mixing with another solution, which stops the reaction (chemical quenching), quickly lowering the temperature (freeze quenching) or even by exposing the sample to light of a certain wavelength (optical quenching).".
- Stopped_flow wikiPageID "5679766".
- Stopped_flow wikiPageLength "2168".
- Stopped_flow wikiPageOutDegree "11".
- Stopped_flow wikiPageRevisionID "662072469".
- Stopped_flow wikiPageWikiLink Category:Biophysics.
- Stopped_flow wikiPageWikiLink Category:Chemical_kinetics.
- Stopped_flow wikiPageWikiLink Chemical_kinetics.
- Stopped_flow wikiPageWikiLink Circular_dichroism.
- Stopped_flow wikiPageWikiLink Fluorescence_spectroscopy.
- Stopped_flow wikiPageWikiLink Protein_folding.
- Stopped_flow wikiPageWikiLink Quenching.
- Stopped_flow wikiPageWikiLink Reagent.
- Stopped_flow wikiPageWikiLink Scattering.
- Stopped_flow wikiPageWikiLink Spectroscopy.
- Stopped_flow wikiPageWikiLink Syringe_driver.
- Stopped_flow wikiPageWikiLinkText "Stopped flow".
- Stopped_flow wikiPageWikiLinkText "stopped flow".
- Stopped_flow wikiPageWikiLinkText "stopped-flow".
- Stopped_flow file "S06033".
- Stopped_flow hasPhotoCollection Stopped_flow.
- Stopped_flow title "stopped flow".
- Stopped_flow wikiPageUsesTemplate Template:Chemistry-stub.
- Stopped_flow wikiPageUsesTemplate Template:GoldBookRef.
- Stopped_flow subject Category:Biophysics.
- Stopped_flow subject Category:Chemical_kinetics.
- Stopped_flow hypernym Device.
- Stopped_flow type Device.
- Stopped_flow type Biophysic.
- Stopped_flow comment "A stopped flow instrument is a rapid mixing device used to study the chemical kinetics of fast reactions in solution. In stopped-flow technique, the solutions are forced from syringes into a mixing chamber. After a very short period of flow, perhaps a few ms, the flow is stopped suddenly when the observation cell is filled by an opposing piston that is linked to a sensing switch that triggers the measuring device.".
- Stopped_flow label "Stopped flow".
- Stopped_flow sameAs Stopped-Flow-Methode.
- Stopped_flow sameAs m.0dzs01.
- Stopped_flow sameAs Q2351880.
- Stopped_flow sameAs Q2351880.
- Stopped_flow wasDerivedFrom Stopped_flow?oldid=662072469.
- Stopped_flow isPrimaryTopicOf Stopped_flow.