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- QMCF_Technology abstract "QMCF Technology is an episomal protein expression system that uses genetically modified mammalian cells and specially designed plasmids. QMCF plasmids carry a combination of regulatory sequences from mouse polyomavirus (Py) DNA replication origin which in combination with Epstein-Barr virus (EBV) EBNA-1 protein binding site as nuclear retention elements ensure stable propagation of plasmids in mammalian cells. In addition the vetors carry the selection marker operational for selection of plasmid carrying bacteria and QMCF cells, bacterial ColE1 origin of replication, and cassette for expression of protein of interest. QMCF cell lines express Large-T antigen and EBNA-1 proteins which bind the viral sequnces on the QMCF plasmid and hence support plasmid replication and maintenance in the cells. QMCF Technology has several important differences compared to commonly known transient expression and stable cell line expression systems. Unlike in transient expression system, QMCF Technology enables to maintain episomally replicating QMCF plasmids inside the cells for up to 50 days thus providing an option for production phase of 2–3 weeks. Therefore the production levels of QMCF Technology are higher (up to 1g/L). Another difference is the option of establishing expression cell banks within one week, which is not feasible with transient system. Compared to usage of stable cell line, QMCF technology is a rapid method leaving out time-consuming clone selection step during cell line development.".
- QMCF_Technology wikiPageExternalLink www.icosagen.com.
- QMCF_Technology wikiPageExternalLink qmcf-technology-licensing.
- QMCF_Technology wikiPageExternalLink application?number=EP06706887.
- QMCF_Technology wikiPageID "27975505".
- QMCF_Technology wikiPageLength "3717".
- QMCF_Technology wikiPageOutDegree "3".
- QMCF_Technology wikiPageRevisionID "678218159".
- QMCF_Technology wikiPageWikiLink Category:Biotechnology.
- QMCF_Technology wikiPageWikiLink EBNA-1.
- QMCF_Technology wikiPageWikiLink Epstein–Barr_virus_nuclear_antigen_1.
- QMCF_Technology wikiPageWikiLink Protein_expression.
- QMCF_Technology wikiPageWikiLink Protein_production_(biotechnology).
- QMCF_Technology wikiPageWikiLinkText "QMCF Technology".
- QMCF_Technology hasPhotoCollection QMCF_Technology.
- QMCF_Technology wikiPageUsesTemplate Template:Cite_journal.
- QMCF_Technology wikiPageUsesTemplate Template:Reflist.
- QMCF_Technology wikiPageUsesTemplate Template:Underlinked.
- QMCF_Technology subject Category:Biotechnology.
- QMCF_Technology hypernym System.
- QMCF_Technology type Article.
- QMCF_Technology type Article.
- QMCF_Technology comment "QMCF Technology is an episomal protein expression system that uses genetically modified mammalian cells and specially designed plasmids. QMCF plasmids carry a combination of regulatory sequences from mouse polyomavirus (Py) DNA replication origin which in combination with Epstein-Barr virus (EBV) EBNA-1 protein binding site as nuclear retention elements ensure stable propagation of plasmids in mammalian cells.".
- QMCF_Technology label "QMCF Technology".
- QMCF_Technology sameAs تقنية_QMCF.
- QMCF_Technology sameAs m.0ch2qjj.
- QMCF_Technology sameAs Q7265488.
- QMCF_Technology sameAs Q7265488.
- QMCF_Technology wasDerivedFrom QMCF_Technology?oldid=678218159.
- QMCF_Technology isPrimaryTopicOf QMCF_Technology.