Matches in DBpedia 2015-10 for { <http://dbpedia.org/resource/Protein_tag> ?p ?o }
- Protein_tag abstract "Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity technique. These include chitin binding protein (CBP), maltose binding protein (MBP), and glutathione-S-transferase (GST). The poly(His) tag is a widely used protein tag; it binds to metal matrices.Solubilization tags are used, especially for recombinant proteins expressed in chaperone-deficient species such as E. coli, to assist in the proper folding in proteins and keep them from precipitating. These include thioredoxin (TRX) and poly(NANP). Some affinity tags have a dual role as a solubilization agent, such as MBP, and GST.Chromatography tags are used to alter chromatographic properties of the protein to afford different resolution across a particular separation technique. Often, these consist of polyanionic amino acids, such as FLAG-tag.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, and HA-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.Fluorescence tags are used to give visual readout on a protein. GFP and its variants are the most commonly used fluorescence tags. More advanced applications of GFP include using it as a folding reporter (fluorescent if folded, colorless if not).Protein tags find many other usages, such as specific enzymatic modification (such as biotin ligase tags) and chemical modification (FlAsH) tag. Often tags are combined to produce multifunctional modifications of the protein. However, with the addition of each tag comes the risk that the native function of the protein may be abolished or compromised by interactions with the tag. Therefore, after purification, tags are commonly removed by specific proteolysis (e.g. by TEV protease, Thrombin, Factor Xa or Enteropeptidase)".
- Protein_tag wikiPageID "2520961".
- Protein_tag wikiPageLength "8514".
- Protein_tag wikiPageOutDegree "55".
- Protein_tag wikiPageRevisionID "682892293".
- Protein_tag wikiPageWikiLink Affinity_chromatography.
- Protein_tag wikiPageWikiLink Affinity_purification.
- Protein_tag wikiPageWikiLink Antibodies.
- Protein_tag wikiPageWikiLink Antibody.
- Protein_tag wikiPageWikiLink BCCP.
- Protein_tag wikiPageWikiLink Biotin_carboxyl_carrier_protein.
- Protein_tag wikiPageWikiLink Biotinylation.
- Protein_tag wikiPageWikiLink Calmodulin-tag.
- Protein_tag wikiPageWikiLink Category:Biochemistry_methods.
- Protein_tag wikiPageWikiLink Category:Laboratory_techniques.
- Protein_tag wikiPageWikiLink Category:Molecular_biology.
- Protein_tag wikiPageWikiLink Category:Proteins.
- Protein_tag wikiPageWikiLink E._coli.
- Protein_tag wikiPageWikiLink Enteropeptidase.
- Protein_tag wikiPageWikiLink Epitope.
- Protein_tag wikiPageWikiLink Escherichia_coli.
- Protein_tag wikiPageWikiLink FLAG-tag.
- Protein_tag wikiPageWikiLink Factor_X.
- Protein_tag wikiPageWikiLink Factor_Xa.
- Protein_tag wikiPageWikiLink Fragment_crystallizable_region.
- Protein_tag wikiPageWikiLink Glutathione-S-transferase.
- Protein_tag wikiPageWikiLink Glutathione_S-transferase.
- Protein_tag wikiPageWikiLink Green_fluorescent_protein.
- Protein_tag wikiPageWikiLink HA-tag.
- Protein_tag wikiPageWikiLink His-tag.
- Protein_tag wikiPageWikiLink Hydrolase.
- Protein_tag wikiPageWikiLink Immunofluorescence.
- Protein_tag wikiPageWikiLink Immunoprecipitation.
- Protein_tag wikiPageWikiLink Intein.
- Protein_tag wikiPageWikiLink Isopeptag.
- Protein_tag wikiPageWikiLink Maltose-binding_protein.
- Protein_tag wikiPageWikiLink Maltose_binding_protein.
- Protein_tag wikiPageWikiLink Myc-tag.
- Protein_tag wikiPageWikiLink Nus-tag.
- Protein_tag wikiPageWikiLink Polyhistidine-tag.
- Protein_tag wikiPageWikiLink Promega.
- Protein_tag wikiPageWikiLink Protein_array.
- Protein_tag wikiPageWikiLink Protein_microarray.
- Protein_tag wikiPageWikiLink Proteinogenic_amino_acid.
- Protein_tag wikiPageWikiLink Recombinant_DNA.
- Protein_tag wikiPageWikiLink Recombinant_protein.
- Protein_tag wikiPageWikiLink S-tag.
- Protein_tag wikiPageWikiLink SBP-tag.
- Protein_tag wikiPageWikiLink Softag_1.
- Protein_tag wikiPageWikiLink Softag_3.
- Protein_tag wikiPageWikiLink SpyTag.
- Protein_tag wikiPageWikiLink Strep-tag.
- Protein_tag wikiPageWikiLink Streptavidin.
- Protein_tag wikiPageWikiLink TC_tag.
- Protein_tag wikiPageWikiLink TEV_protease.
- Protein_tag wikiPageWikiLink Thioredoxin.
- Protein_tag wikiPageWikiLink Thrombin.
- Protein_tag wikiPageWikiLink Ty_tag.
- Protein_tag wikiPageWikiLink V5-tag.
- Protein_tag wikiPageWikiLink V5_tag.
- Protein_tag wikiPageWikiLink Western_blot.
- Protein_tag wikiPageWikiLink Western_blotting.
- Protein_tag wikiPageWikiLink Xpress_tag.
- Protein_tag wikiPageWikiLinkText "Protein purification tags".
- Protein_tag wikiPageWikiLinkText "Protein tag".
- Protein_tag wikiPageWikiLinkText "Protein-tag".
- Protein_tag wikiPageWikiLinkText "affinity tags".
- Protein_tag wikiPageWikiLinkText "biological tag".
- Protein_tag wikiPageWikiLinkText "protein labeling".
- Protein_tag wikiPageWikiLinkText "protein tag".
- Protein_tag wikiPageWikiLinkText "purification tag".
- Protein_tag wikiPageWikiLinkText "tag sequence".
- Protein_tag wikiPageWikiLinkText "tag".
- Protein_tag wikiPageWikiLinkText "tags".
- Protein_tag hasPhotoCollection Protein_tag.
- Protein_tag wikiPageUsesTemplate Template:Protein_methods.
- Protein_tag wikiPageUsesTemplate Template:Reflist.
- Protein_tag subject Category:Biochemistry_methods.
- Protein_tag subject Category:Laboratory_techniques.
- Protein_tag subject Category:Molecular_biology.
- Protein_tag subject Category:Proteins.
- Protein_tag hypernym Sequences.
- Protein_tag type Article.
- Protein_tag type Biomolecule.
- Protein_tag type Protein.
- Protein_tag type Article.
- Protein_tag type Biomolecule.
- Protein_tag type Laboratory.
- Protein_tag type Method.
- Protein_tag type Nutrient.
- Protein_tag type Technique.
- Protein_tag comment "Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity technique. These include chitin binding protein (CBP), maltose binding protein (MBP), and glutathione-S-transferase (GST).".
- Protein_tag label "Protein tag".
- Protein_tag sameAs Protein-Tag.
- Protein_tag sameAs Étiquette_(biologie).
- Protein_tag sameAs Etichetta_proteica.
- Protein_tag sameAs タンパク質タグ.
- Protein_tag sameAs 표지단백질.
- Protein_tag sameAs Znacznik_(inżynieria_genetyczna).
- Protein_tag sameAs m.07kmy7.