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- Protein_mass_spectrometry abstract "Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as "top-down" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called "bottom-up" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.".
- Protein_mass_spectrometry thumbnail Mass_Spectrometer.jpg?width=300.
- Protein_mass_spectrometry wikiPageID "13250438".
- Protein_mass_spectrometry wikiPageLength "20870".
- Protein_mass_spectrometry wikiPageOutDegree "64".
- Protein_mass_spectrometry wikiPageRevisionID "682250094".
- Protein_mass_spectrometry wikiPageWikiLink Alanine.
- Protein_mass_spectrometry wikiPageWikiLink Bottom-up_proteomics.
- Protein_mass_spectrometry wikiPageWikiLink Carbon.
- Protein_mass_spectrometry wikiPageWikiLink Category:Mass_spectrometry.
- Protein_mass_spectrometry wikiPageWikiLink Category:Proteomics.
- Protein_mass_spectrometry wikiPageWikiLink De_novo_peptide_sequencing.
- Protein_mass_spectrometry wikiPageWikiLink De_novo_sequencing.
- Protein_mass_spectrometry wikiPageWikiLink Deuterium.
- Protein_mass_spectrometry wikiPageWikiLink ESI.
- Protein_mass_spectrometry wikiPageWikiLink Electron-capture_dissociation.
- Protein_mass_spectrometry wikiPageWikiLink Electron_capture_dissociation.
- Protein_mass_spectrometry wikiPageWikiLink Electrospray.
- Protein_mass_spectrometry wikiPageWikiLink Electrospray_ionization.
- Protein_mass_spectrometry wikiPageWikiLink Fourier_transform_ion_cyclotron_resonance.
- Protein_mass_spectrometry wikiPageWikiLink Franz_Hillenkamp.
- Protein_mass_spectrometry wikiPageWikiLink High-performance_liquid_chromatography.
- Protein_mass_spectrometry wikiPageWikiLink High_performance_liquid_chromatography.
- Protein_mass_spectrometry wikiPageWikiLink Hydrogen-deuterium_exchange.
- Protein_mass_spectrometry wikiPageWikiLink Hydrogen–deuterium_exchange.
- Protein_mass_spectrometry wikiPageWikiLink ITRAQ.
- Protein_mass_spectrometry wikiPageWikiLink In-gel_digestion.
- Protein_mass_spectrometry wikiPageWikiLink Isobaric_tag_for_relative_and_absolute_quantitation.
- Protein_mass_spectrometry wikiPageWikiLink Isoelectric_focusing.
- Protein_mass_spectrometry wikiPageWikiLink Isoleucine.
- Protein_mass_spectrometry wikiPageWikiLink Isotope.
- Protein_mass_spectrometry wikiPageWikiLink Isotope-coded_affinity_tag.
- Protein_mass_spectrometry wikiPageWikiLink John_Bennett_Fenn.
- Protein_mass_spectrometry wikiPageWikiLink John_Fenn_(chemist).
- Protein_mass_spectrometry wikiPageWikiLink Koichi_Tanaka.
- Protein_mass_spectrometry wikiPageWikiLink Kurt_Wüthrich.
- Protein_mass_spectrometry wikiPageWikiLink Leucine.
- Protein_mass_spectrometry wikiPageWikiLink MALDI.
- Protein_mass_spectrometry wikiPageWikiLink Malcolm_Dole.
- Protein_mass_spectrometry wikiPageWikiLink Mass.
- Protein_mass_spectrometry wikiPageWikiLink Mass_spectrometry.
- Protein_mass_spectrometry wikiPageWikiLink ionization.
- Protein_mass_spectrometry wikiPageWikiLink Michael_Karas.
- Protein_mass_spectrometry wikiPageWikiLink Nitrogen.
- Protein_mass_spectrometry wikiPageWikiLink Nobel_Prize_in_Chemistry.
- Protein_mass_spectrometry wikiPageWikiLink Peptide.
- Protein_mass_spectrometry wikiPageWikiLink Peptide_mass_fingerprinting.
- Protein_mass_spectrometry wikiPageWikiLink Peptide_spectral_library.
- Protein_mass_spectrometry wikiPageWikiLink Peptides.
- Protein_mass_spectrometry wikiPageWikiLink Protease.
- Protein_mass_spectrometry wikiPageWikiLink Protein.
- Protein_mass_spectrometry wikiPageWikiLink Protein_structure.
- Protein_mass_spectrometry wikiPageWikiLink Proteins.
- Protein_mass_spectrometry wikiPageWikiLink Proteogenomics.
- Protein_mass_spectrometry wikiPageWikiLink Quadrupole_ion_trap.
- Protein_mass_spectrometry wikiPageWikiLink Quantitative_proteomics.
- Protein_mass_spectrometry wikiPageWikiLink SILAC.
- Protein_mass_spectrometry wikiPageWikiLink Stable_isotope_labeling_by_amino_acids_in_cell_culture.
- Protein_mass_spectrometry wikiPageWikiLink Tandem_mass_spectrometry.
- Protein_mass_spectrometry wikiPageWikiLink Time-of-flight.
- Protein_mass_spectrometry wikiPageWikiLink Time_of_flight.
- Protein_mass_spectrometry wikiPageWikiLink Top-down_proteomics.
- Protein_mass_spectrometry wikiPageWikiLink Trypsin.
- Protein_mass_spectrometry wikiPageWikiLink Tryptophan.
- Protein_mass_spectrometry wikiPageWikiLink Two-dimensional_gel_electrophoresis.
- Protein_mass_spectrometry wikiPageWikiLink File:Mass_Spectrometer.jpg.
- Protein_mass_spectrometry wikiPageWikiLink File:Mass_spectrometry_protocol.png.
- Protein_mass_spectrometry wikiPageWikiLink File:PeptideMSMS.jpg.
- Protein_mass_spectrometry wikiPageWikiLink File:Quantitative_mass_spectrometry.svg.
- Protein_mass_spectrometry wikiPageWikiLinkText "Protein mass spectrometry".
- Protein_mass_spectrometry wikiPageWikiLinkText "mass spectrometry".
- Protein_mass_spectrometry wikiPageWikiLinkText "protein mass spectrometry".
- Protein_mass_spectrometry wikiPageWikiLinkText "proteomics".
- Protein_mass_spectrometry date "May 2015".
- Protein_mass_spectrometry hasPhotoCollection Protein_mass_spectrometry.
- Protein_mass_spectrometry reason "Orthographic errors detract from credibility of factual information".
- Protein_mass_spectrometry wikiPageUsesTemplate Template:Analytical_chemistry.
- Protein_mass_spectrometry wikiPageUsesTemplate Template:Cleanup.
- Protein_mass_spectrometry wikiPageUsesTemplate Template:Main.
- Protein_mass_spectrometry wikiPageUsesTemplate Template:Reflist.
- Protein_mass_spectrometry subject Category:Mass_spectrometry.
- Protein_mass_spectrometry subject Category:Proteomics.
- Protein_mass_spectrometry type Article.
- Protein_mass_spectrometry type Article.
- Protein_mass_spectrometry type Omic.
- Protein_mass_spectrometry type Proteomic.
- Protein_mass_spectrometry comment "Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins.".
- Protein_mass_spectrometry label "Protein mass spectrometry".
- Protein_mass_spectrometry sameAs Massaspektrometria_proteiinianalytiikassa.
- Protein_mass_spectrometry sameAs m.03b_9ss.
- Protein_mass_spectrometry sameAs Q7251488.
- Protein_mass_spectrometry sameAs Q7251488.
- Protein_mass_spectrometry wasDerivedFrom Protein_mass_spectrometry?oldid=682250094.
- Protein_mass_spectrometry depiction Mass_Spectrometer.jpg.
- Protein_mass_spectrometry isPrimaryTopicOf Protein_mass_spectrometry.