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- Protein_L abstract "Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind immunoglobulins through L chain interaction, from which the name was suggested. It consists of 719 amino acid residues. The molecular weight of Protein L purified from the cell walls of Peptostreptoccus magnus was first estimated as 95kD by SDS-PAGE in the presence of reducing agent 2-mercaptoethanol, while the molecular weight was determined to 76kD by gel chromotography in the presence of 6 M guanidine HCl. Protein L does not contain any interchain disulfide loops, nor does it consist of disulfide-linked subunits. It was an acidic molecule with a PI of 4.0. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains. In humans and mice, most antibody molecules contain kappa (κ) light chains and the remainder have lambda (λ) light chains. Protein L is only effective in binding certain subtypes of kappa light chains. For example, it binds human VκI, VκIII and VκIV subtypes but does not bind the VκII subtype. Binding of mouse immunoglobulins is restricted to those having VκI light chains.Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLκ-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.".
- Protein_L symbol "PpL".
- Protein_L thumbnail Protein_L_1MHH.png?width=300.
- Protein_L wikiPageID "10919113".
- Protein_L wikiPageLength "5875".
- Protein_L wikiPageOutDegree "26".
- Protein_L wikiPageRevisionID "633314728".
- Protein_L wikiPageWikiLink Antibodies.
- Protein_L wikiPageWikiLink Antibody.
- Protein_L wikiPageWikiLink Ascites.
- Protein_L wikiPageWikiLink Blood_serum.
- Protein_L wikiPageWikiLink Bovinae.
- Protein_L wikiPageWikiLink Bovine.
- Protein_L wikiPageWikiLink Category:Proteins.
- Protein_L wikiPageWikiLink Cell_culture.
- Protein_L wikiPageWikiLink IgA.
- Protein_L wikiPageWikiLink IgD.
- Protein_L wikiPageWikiLink IgE.
- Protein_L wikiPageWikiLink IgG.
- Protein_L wikiPageWikiLink IgM.
- Protein_L wikiPageWikiLink Immunoglobulin_A.
- Protein_L wikiPageWikiLink Immunoglobulin_D.
- Protein_L wikiPageWikiLink Immunoglobulin_E.
- Protein_L wikiPageWikiLink Immunoglobulin_G.
- Protein_L wikiPageWikiLink Immunoglobulin_M.
- Protein_L wikiPageWikiLink Immunoglobulin_light_chain.
- Protein_L wikiPageWikiLink Immunoprecipitation.
- Protein_L wikiPageWikiLink Monoclonal_antibodies.
- Protein_L wikiPageWikiLink Monoclonal_antibody.
- Protein_L wikiPageWikiLink Peptostreptococcus.
- Protein_L wikiPageWikiLink Peptostreptococcus_magnus.
- Protein_L wikiPageWikiLink Precipitation_(chemistry).
- Protein_L wikiPageWikiLink Protein.
- Protein_L wikiPageWikiLink Protein_A.
- Protein_L wikiPageWikiLink G.
- Protein_L wikiPageWikiLink Protein_G.
- Protein_L wikiPageWikiLink ScFv.
- Protein_L wikiPageWikiLink Serum_(blood).
- Protein_L wikiPageWikiLink Single-chain_variable_fragment.
- Protein_L wikiPageWikiLink Supernatant.
- Protein_L wikiPageWikiLinkText "Protein L".
- Protein_L wikiPageWikiLinkText "Protein L".
- Protein_L wikiPageWikiLinkText "protein L".
- Protein_L caption "Structure of protein L binding to the light light chain of a murine Fab.".
- Protein_L hasPhotoCollection Protein_L.
- Protein_L interpro "IPR003147".
- Protein_L name "Protein L b1 domain".
- Protein_L pdb ", , , , , , , , , , , , , , , , ,".
- Protein_L pfam "PF02246".
- Protein_L scop "1".
- Protein_L symbol "PpL".
- Protein_L wikiPageUsesTemplate Template:Pfam_box.
- Protein_L wikiPageUsesTemplate Template:Reflist.
- Protein_L subject Category:Proteins.
- Protein_L type Biomolecule.
- Protein_L type Protein.
- Protein_L type Biomolecule.
- Protein_L type Chemical.
- Protein_L type Nutrient.
- Protein_L type Thing.
- Protein_L type Q206229.
- Protein_L type Q8054.
- Protein_L comment "Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind immunoglobulins through L chain interaction, from which the name was suggested. It consists of 719 amino acid residues.".
- Protein_L label "Protein L".
- Protein_L sameAs Protein_L.
- Protein_L sameAs m.02qv03z.
- Protein_L sameAs Q7251440.
- Protein_L sameAs Q7251440.
- Protein_L wasDerivedFrom Protein_L?oldid=633314728.
- Protein_L depiction Protein_L_1MHH.png.
- Protein_L isPrimaryTopicOf Protein_L.