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- Ponceau_S abstract "Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot-sequencing. It is also easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.".
- Ponceau_S thumbnail Ponceau_S.svg?width=300.
- Ponceau_S wikiPageID "3208346".
- Ponceau_S wikiPageLength "2886".
- Ponceau_S wikiPageOutDegree "17".
- Ponceau_S wikiPageRevisionID "670394895".
- Ponceau_S wikiPageWikiLink 5-Sulfosalicylic_acid.
- Ponceau_S wikiPageWikiLink Acetic_acid.
- Ponceau_S wikiPageWikiLink Azo_compound.
- Ponceau_S wikiPageWikiLink Category:Azo_dyes.
- Ponceau_S wikiPageWikiLink Category:Protein_methods.
- Ponceau_S wikiPageWikiLink Cellulose_acetate.
- Ponceau_S wikiPageWikiLink Coomassie_Brilliant_Blue.
- Ponceau_S wikiPageWikiLink Diazo_dye.
- Ponceau_S wikiPageWikiLink Immunology.
- Ponceau_S wikiPageWikiLink Nitrocellulose.
- Ponceau_S wikiPageWikiLink Polyvinylidene_fluoride.
- Ponceau_S wikiPageWikiLink Ponceau.
- Ponceau_S wikiPageWikiLink Protein.
- Ponceau_S wikiPageWikiLink Staining.
- Ponceau_S wikiPageWikiLink Staining_(biology).
- Ponceau_S wikiPageWikiLink Sulfosalicylic_acid.
- Ponceau_S wikiPageWikiLink Trichloroacetic_acid.
- Ponceau_S wikiPageWikiLink Western_blot.
- Ponceau_S wikiPageWikiLink File:PonceauMembrane.png.
- Ponceau_S wikiPageWikiLinkText "Ponceau S".
- Ponceau_S hasPhotoCollection Ponceau_S.
- Ponceau_S imagefile "Ponceau S.svg".
- Ponceau_S imagesize "200".
- Ponceau_S wikiPageUsesTemplate Template:Chembox.
- Ponceau_S wikiPageUsesTemplate Template:Clear-left.
- Ponceau_S wikiPageUsesTemplate Template:Reflist.
- Ponceau_S subject Category:Azo_dyes.
- Ponceau_S subject Category:Protein_methods.
- Ponceau_S hypernym Salt.
- Ponceau_S type Article.
- Ponceau_S type ChemicalCompound.
- Ponceau_S type ChemicalSubstance.
- Ponceau_S type Article.
- Ponceau_S type Method.
- Ponceau_S type ChemicalObject.
- Ponceau_S type Thing.
- Ponceau_S type Q11173.
- Ponceau_S comment "Ponceau S, Acid Red 112, or C.I. 27195 is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting), as well as on cellulose acetate membranes.".
- Ponceau_S label "Ponceau S".
- Ponceau_S sameAs Ponceau_S.
- Ponceau_S sameAs m.08zdmh.
- Ponceau_S sameAs Q2103492.
- Ponceau_S sameAs Q2103492.
- Ponceau_S wasDerivedFrom Ponceau_S?oldid=670394895.
- Ponceau_S depiction Ponceau_S.svg.
- Ponceau_S isPrimaryTopicOf Ponceau_S.