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- Okazaki_fragments abstract "Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication. They are complementary to the lagging template strand, together forming short double-stranded DNA sections. Okazaki fragments are between 1000 and 2000 nucleotides long in Escherichia coli and are approximately 150 nucleotides long in eukaryotes. They are separated by ~10-nucleotide RNA primers and are unligated until RNA primers are removed, followed by enzyme ligase connecting (ligating) the two Okazaki fragments into one continuous newly synthesized complementary strand. On the leading strand DNA replication proceeds continuously along the DNA molecule as the parent double-stranded DNA is unwound, but on the lagging strand the new DNA is made in installments, which are later joined together by a DNA ligase enzyme. This is because the enzymes that synthesise the new DNA can only work in one direction along the parent DNA molecule. On the leading strand this route is continuous, but on the lagging strand it is discontinuous.DNA is synthesised from 5' to 3', so when copying the 3' to 5' strand, replication is continuous. Phosphodiester links form between the 3' to 5' and nucleotides can be added with the aid of the enzyme DNA polymerase for the continuous leading strand. However, in order to synthesise the lagging strand (the replication fork which is travelling in the opposite direction) synthesis occurs in small sections (100-200 nucleotides at a time in eukaryotes). These new stretches of DNA are called Okazaki fragments and each one requires its own RNA primer. A series of experiments eventually lead to the discovery of Okazaki fragments. The experiments (see below) were conducted during the 1960s with Reiji Okazaki, Tsuneko Okazaki, Kiwako Sakabe and their colleagues during their research on DNA replication of Escherichia coli. In 1966, Kiwako Sakabe and Reiji Okazaki first showed that DNA replication was a discontinuous process involving fragments. The fragments were further investigated by the researchers and their colleagues through their research including the study on bacteriophage DNA replication in Escherichia coli.".
- Okazaki_fragments thumbnail DNA_replication.svg?width=300.
- Okazaki_fragments wikiPageExternalLink 10-lab.pdf.
- Okazaki_fragments wikiPageID "506315".
- Okazaki_fragments wikiPageLength "24140".
- Okazaki_fragments wikiPageOutDegree "59".
- Okazaki_fragments wikiPageRevisionID "683034273".
- Okazaki_fragments wikiPageWikiLink Autonomously_replicating_sequence.
- Okazaki_fragments wikiPageWikiLink Autonomously_replicating_sequences.
- Okazaki_fragments wikiPageWikiLink Bacteriophage.
- Okazaki_fragments wikiPageWikiLink Bacteriophage_T4.
- Okazaki_fragments wikiPageWikiLink Category:DNA_replication.
- Okazaki_fragments wikiPageWikiLink DNA.
- Okazaki_fragments wikiPageWikiLink DNA_ligase.
- Okazaki_fragments wikiPageWikiLink DNA_polymerase.
- Okazaki_fragments wikiPageWikiLink DNA_replication.
- Okazaki_fragments wikiPageWikiLink Directionality_(molecular_biology).
- Okazaki_fragments wikiPageWikiLink E._Coli.
- Okazaki_fragments wikiPageWikiLink Enterobacteria_phage_T4.
- Okazaki_fragments wikiPageWikiLink Enzyme.
- Okazaki_fragments wikiPageWikiLink Escherichia_coli.
- Okazaki_fragments wikiPageWikiLink Eukaryote.
- Okazaki_fragments wikiPageWikiLink Eukaryotes.
- Okazaki_fragments wikiPageWikiLink Exonuclease.
- Okazaki_fragments wikiPageWikiLink FEN1.
- Okazaki_fragments wikiPageWikiLink Flap_structure-specific_endonuclease_1.
- Okazaki_fragments wikiPageWikiLink Hypoplasia.
- Okazaki_fragments wikiPageWikiLink Leading_strand.
- Okazaki_fragments wikiPageWikiLink Ligase.
- Okazaki_fragments wikiPageWikiLink Nucleotide.
- Okazaki_fragments wikiPageWikiLink Nucleotides.
- Okazaki_fragments wikiPageWikiLink Pancytopenia.
- Okazaki_fragments wikiPageWikiLink Primase.
- Okazaki_fragments wikiPageWikiLink Primer_(molecular_biology).
- Okazaki_fragments wikiPageWikiLink Prokaryote.
- Okazaki_fragments wikiPageWikiLink Prokaryotes.
- Okazaki_fragments wikiPageWikiLink Proliferating_cell_nuclear_antigen.
- Okazaki_fragments wikiPageWikiLink Pulse_labelling.
- Okazaki_fragments wikiPageWikiLink RNA_primer.
- Okazaki_fragments wikiPageWikiLink Reiji_Okazaki.
- Okazaki_fragments wikiPageWikiLink Replication_fork.
- Okazaki_fragments wikiPageWikiLink Tsuneko_Okazaki.
- Okazaki_fragments wikiPageWikiLink File:DNA-Okazaki-Fragment-prelim.PNG.
- Okazaki_fragments wikiPageWikiLink File:DNA_replication.svg.
- Okazaki_fragments wikiPageWikiLink File:Dna-split.png.
- Okazaki_fragments wikiPageWikiLink File:RNA_primer.png.
- Okazaki_fragments wikiPageWikiLinkText "Okazaki fragment".
- Okazaki_fragments wikiPageWikiLinkText "Okazaki fragments".
- Okazaki_fragments hasPhotoCollection Okazaki_fragments.
- Okazaki_fragments wikiPageUsesTemplate Template:DNA_replication.
- Okazaki_fragments wikiPageUsesTemplate Template:Educational_assignment.
- Okazaki_fragments wikiPageUsesTemplate Template:Main.
- Okazaki_fragments wikiPageUsesTemplate Template:MeshName.
- Okazaki_fragments wikiPageUsesTemplate Template:Refbegin.
- Okazaki_fragments wikiPageUsesTemplate Template:Refend.
- Okazaki_fragments wikiPageUsesTemplate Template:Reflist.
- Okazaki_fragments subject Category:DNA_replication.
- Okazaki_fragments type Article.
- Okazaki_fragments type Article.
- Okazaki_fragments comment "Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication. They are complementary to the lagging template strand, together forming short double-stranded DNA sections. Okazaki fragments are between 1000 and 2000 nucleotides long in Escherichia coli and are approximately 150 nucleotides long in eukaryotes.".
- Okazaki_fragments label "Okazaki fragments".
- Okazaki_fragments sameAs شظايا_أوكازاكي.
- Okazaki_fragments sameAs Fragment_dOkazaki.
- Okazaki_fragments sameAs Okazakiho_fragment.
- Okazaki_fragments sameAs Okazaki-Fragment.
- Okazaki_fragments sameAs Θραύσματα_του_Οκαζάκι.
- Okazaki_fragments sameAs Fragmento_de_Okazaki.
- Okazaki_fragments sameAs Okazaki_fragment.
- Okazaki_fragments sameAs Fragment_dOkazaki.
- Okazaki_fragments sameAs Fragmento_de_Okazaki.
- Okazaki_fragments sameAs מקטעי_אוקזקי.
- Okazaki_fragments sameAs Fragmen_Okazaki.
- Okazaki_fragments sameAs Frammento_di_Okazaki.
- Okazaki_fragments sameAs 岡崎フラグメント.
- Okazaki_fragments sameAs 오카자키_절편.
- Okazaki_fragments sameAs Okazaki-fragment.
- Okazaki_fragments sameAs Fragment_dOkazaki.
- Okazaki_fragments sameAs Fragmenty_Okazaki.
- Okazaki_fragments sameAs Fragmento_de_Okazaki.
- Okazaki_fragments sameAs m.02jl4z.
- Okazaki_fragments sameAs Фрагменты_Оказаки.
- Okazaki_fragments sameAs Okazakijev_fragment.
- Okazaki_fragments sameAs Okazakiho_fragment.
- Okazaki_fragments sameAs Okazakijev_fragment.
- Okazaki_fragments sameAs Okazaki_parçaları.
- Okazaki_fragments sameAs Фрагменти_Окадзакі.
- Okazaki_fragments sameAs Q845661.
- Okazaki_fragments sameAs Q845661.
- Okazaki_fragments sameAs 岡崎片段.
- Okazaki_fragments wasDerivedFrom Okazaki_fragments?oldid=683034273.
- Okazaki_fragments depiction DNA_replication.svg.
- Okazaki_fragments isPrimaryTopicOf Okazaki_fragments.