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- Microtubule_nucleation abstract "In cell anatomy, Microtubule nucleation is the event that initiates de novo formation of microtubules. These filaments of the cytoskeleton typically form through polymerization of α- and β-tubulin dimers, the basic building blocks of the microtubule, which initially interact to nucleate a seed from which the filament elongates.Microtubule nucleation occurs spontaneously in vitro, with solutions of purified tubulin giving rise to full-length polymers. The tubulin dimers that make up the polymers have an intrinsic capacity to self-aggregate and assemble into cylindrical tubes, provided there is an adequate supply of GTP. The kinetics barriers of such a process however mean that the rate at which microtubules spontaneously nucleate is relatively low.In vivo, cells get around this kinetic barrier by using various proteins to aid microtubule nucleation. The primary pathway by which microtubule nucleation is assisted requires the action of a third type of tubulin, γ-tubulin, which is distinct from the α and β subunits that compose the microtubules themselves. The γ-tubulin combines with several other associated proteins to form a conical structure known as the γ-tubulin ring complex (γ-TuRC). This complex, with its 13-fold symmetry, acts as a scaffold or template for α/β tubulin dimers during the nucleation process—speeding up the assembly of the ring of 13 protofilaments that make up the growing microtubule. The γ-TuRC also acts as a cap of the (−) end while the microtubule continues growth from its (+) end. This cap provides both stability and protection to the microtubule (-) end from enzymes that could lead to its depolymerization, while also inhibiting (-) end growth.The γ-TuRC is typically found as the core functional unit in a microtubule organizing center (MTOC), such as the centrosome in animal cells or the spindle pole bodies in fungi and algae. The γ-TuRCs in the centrosome nucleate an array of microtubules in interphase, which extend their (+)-ends radially outwards into the cytoplasm towards the periphery of the cell. Among its other functions, this radial array is used by microtubule-based motor proteins to transport various cargoes, such as vesicles, to the plasma membrane. In animal cells undergoing mitosis, a similar radial array is generated from two MTOCs called the spindle poles, which produce the bipolar mitotic spindle. Some cells however, such as those of higher plants and oocytes, lack distinct MTOCs and microtubules are nucleated via a non-centrosomal pathway. Other cells, such as neurons, skeletal muscle cells, and epithelial cells, which do have MTOCs, possess arrays of microtubules not associated with a centrosome. These non-centrosomal microtubule arrays can take on various geometries—such as those lead to the long, slender shape of myotubes, the fine protrusions of an axon, or the strongly polarized domains of an epithelial cell. Researchers think that the microtubules in these arrays are generated first by the γ-TuRCs, then transported via motor proteins or treadmilling to their desired location, and finally stabilized in the needed configuration through the action of various anchoring and cross-linking proteins.In the cortical array of plants, as well as in the axons of neurons, scientists believe that microtubules nucleate from existing microtubules via the action of severing enzymes such as katanin. Akin to the action of cofilin in generating actin filament arrays, the severing of microtubules by MAPs creates new (+) ends from which microtubules can grow. In this fashion dynamic arrays of microtubules can be generated without the aid of the γ-TuRC.Recent studies using Xenopus egg extracts have identified a novel form of microtubule nucleation that generates fan-like branching arrays, in which new microtubules grow at an angle off of older microtubules. Researchers suspect that this process involves non-centrosomal γ-TuRCs that bind to the sides of existing microtubules through the augmin complex. This method of microtubule-dependent microtubule nucleation leads to rapid amplification in microtubule number, and creates daughter microtubules with the same polarity as the mother microtubules they branch from. Researchers postulate that such a method could be important in the generation of the mitotic spindle.{Citation needed|date=May 2014}Though the γ-TuRC is the primary protein cells turn to when faced with the task of nucleating microtubules, it is not the only protein postulated to act as a nucleation factor. Several other MAPs assist the γ-TuRC with the nucleation process, while others nucleate microtubules independently of γ-TuRC. In the branching nucleation described above, the addition of TPX2 to the egg extracts led to a dramatic increase in nucleation events—while in other studies, the protein XMAP215, in vitro, nucleated microtubule asters with its depletion in vivo reducing nucleation potential of centrosomes. The microtubule-binding protein doublecortin, in vitro, nucleates microtubules—acting by binding to the side rather than the end of growing microtubules. Thus a family of proteins acting as nucleation factors may be present in cells, lowering, through various mechanisms, the energetic cost of nucleating microtubules.Several proteins are involved in formatting the γ-TuRC and temporal and spatial control of microtubule nucleation. These include, for example, coiled-coil proteins with structural functions and regulatory proteins, such as components of the Ran cycle. NEDD1 recruits the γ-TuRC to the centrosome by binding to γ-tubulin.".
- Microtubule_nucleation wikiPageExternalLink go-0007021.
- Microtubule_nucleation wikiPageExternalLink go-0005874.
- Microtubule_nucleation wikiPageID "9485508".
- Microtubule_nucleation wikiPageLength "9222".
- Microtubule_nucleation wikiPageOutDegree "39".
- Microtubule_nucleation wikiPageRevisionID "675785976".
- Microtubule_nucleation wikiPageWikiLink Algae.
- Microtubule_nucleation wikiPageWikiLink Animal.
- Microtubule_nucleation wikiPageWikiLink Axon.
- Microtubule_nucleation wikiPageWikiLink CKAP5.
- Microtubule_nucleation wikiPageWikiLink Category:Cell_anatomy.
- Microtubule_nucleation wikiPageWikiLink Category:Cytoskeleton.
- Microtubule_nucleation wikiPageWikiLink Cell_anatomy.
- Microtubule_nucleation wikiPageWikiLink Centrosome.
- Microtubule_nucleation wikiPageWikiLink Cofilin.
- Microtubule_nucleation wikiPageWikiLink Coiled-coil.
- Microtubule_nucleation wikiPageWikiLink Coiled_coil.
- Microtubule_nucleation wikiPageWikiLink Cytoskeleton.
- Microtubule_nucleation wikiPageWikiLink De_novo_synthesis.
- Microtubule_nucleation wikiPageWikiLink Doublecortin.
- Microtubule_nucleation wikiPageWikiLink Epithelium.
- Microtubule_nucleation wikiPageWikiLink Fungus.
- Microtubule_nucleation wikiPageWikiLink HAUS3.
- Microtubule_nucleation wikiPageWikiLink In_vitro.
- Microtubule_nucleation wikiPageWikiLink In_vivo.
- Microtubule_nucleation wikiPageWikiLink Interphase.
- Microtubule_nucleation wikiPageWikiLink Katanin.
- Microtubule_nucleation wikiPageWikiLink Microtubule.
- Microtubule_nucleation wikiPageWikiLink Microtubule-associated_protein.
- Microtubule_nucleation wikiPageWikiLink Microtubule_organizing_center.
- Microtubule_nucleation wikiPageWikiLink Microtubules.
- Microtubule_nucleation wikiPageWikiLink Mitosis.
- Microtubule_nucleation wikiPageWikiLink Motor_protein.
- Microtubule_nucleation wikiPageWikiLink Motor_proteins.
- Microtubule_nucleation wikiPageWikiLink Muscle.
- Microtubule_nucleation wikiPageWikiLink NEDD1.
- Microtubule_nucleation wikiPageWikiLink Plant.
- Microtubule_nucleation wikiPageWikiLink Polymerization.
- Microtubule_nucleation wikiPageWikiLink Ran_(biology).
- Microtubule_nucleation wikiPageWikiLink Ran_(gene).
- Microtubule_nucleation wikiPageWikiLink Spindle_apparatus.
- Microtubule_nucleation wikiPageWikiLink Spindle_pole.
- Microtubule_nucleation wikiPageWikiLink Spindle_pole_body.
- Microtubule_nucleation wikiPageWikiLink TPX2.
- Microtubule_nucleation wikiPageWikiLink TUBG1.
- Microtubule_nucleation wikiPageWikiLink Treadmilling.
- Microtubule_nucleation wikiPageWikiLink Tubulin.
- Microtubule_nucleation wikiPageWikiLink XMAP215.
- Microtubule_nucleation wikiPageWikiLinkText "Microtubule nucleation".
- Microtubule_nucleation wikiPageWikiLinkText "Nucleation".
- Microtubule_nucleation wikiPageWikiLinkText "TUBG1".
- Microtubule_nucleation wikiPageWikiLinkText "gamma-tubulin ring complex".
- Microtubule_nucleation wikiPageWikiLinkText "microtubule nucleation".
- Microtubule_nucleation wikiPageWikiLinkText "nucleated".
- Microtubule_nucleation wikiPageWikiLinkText "nucleates".
- Microtubule_nucleation wikiPageWikiLinkText "nucleation".
- Microtubule_nucleation wikiPageWikiLinkText "γ-tubulin ring complexes".
- Microtubule_nucleation hasPhotoCollection Microtubule_nucleation.
- Microtubule_nucleation wikiPageUsesTemplate Template:Cytoskeletal_Proteins.
- Microtubule_nucleation wikiPageUsesTemplate Template:Reflist.
- Microtubule_nucleation subject Category:Cell_anatomy.
- Microtubule_nucleation subject Category:Cytoskeleton.
- Microtubule_nucleation hypernym Event.
- Microtubule_nucleation type Article.
- Microtubule_nucleation type SportsEvent.
- Microtubule_nucleation type Article.
- Microtubule_nucleation comment "In cell anatomy, Microtubule nucleation is the event that initiates de novo formation of microtubules. These filaments of the cytoskeleton typically form through polymerization of α- and β-tubulin dimers, the basic building blocks of the microtubule, which initially interact to nucleate a seed from which the filament elongates.Microtubule nucleation occurs spontaneously in vitro, with solutions of purified tubulin giving rise to full-length polymers.".
- Microtubule_nucleation label "Microtubule nucleation".
- Microtubule_nucleation sameAs m.028bsmk.
- Microtubule_nucleation sameAs Q17152528.
- Microtubule_nucleation sameAs Q17152528.
- Microtubule_nucleation wasDerivedFrom Microtubule_nucleation?oldid=675785976.
- Microtubule_nucleation isPrimaryTopicOf Microtubule_nucleation.