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- Lipofectamine abstract "Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. Lipofectamine reagent contains lipid subunits that can form liposomes in an aqueous environment, which entrap the transfection materials, i.e. DNA plasmids. Lipofectamine is a cationic liposome formulation, which complexes with negatively charged nucleic acid molecules to allow them to overcome the electrostatic repulsion of the cell membrane. Lipofectamine's cationic lipid molecules are formulated with a neutral co-lipid (helper lipid). The DNA-containing liposomes (with positive charge on their surfaces) can fuse with the negatively charged plasma membrane of living cells, due to the neutral co-lipid mediating fusion of the liposome with the cell membrane, allowing nucleic acid to cross into the cytoplasm and contents to be available to the cell for replication or expression.In order for a cell to express this transgene, the nucleic acid must reach the nucleus of the cell to begin transcription. This process involves many risks. The transfected genetic material may never reach the nucleus in the first place, instead being disrupted somewhere along the delivery process. In dividing cells, the material may reach the nucleus by being trapped in the reassembling nuclear envelope following mitosis. But also in non-dividing cells, research has shown that Lipofectamine improves the efficiency of transfection, which suggests that it additionally helps the transfected genetic material penetrate the intact nuclear envelope.This method of transfection was invented by Dr. Yongliang Chu at Life Technologies, Inc.".
- Lipofectamine wikiPageID "9361398".
- Lipofectamine wikiPageLength "2974".
- Lipofectamine wikiPageOutDegree "26".
- Lipofectamine wikiPageRevisionID "662964953".
- Lipofectamine wikiPageWikiLink Category:Gene_delivery.
- Lipofectamine wikiPageWikiLink Category:Molecular_biology.
- Lipofectamine wikiPageWikiLink Cationic_liposome.
- Lipofectamine wikiPageWikiLink Cell_biology.
- Lipofectamine wikiPageWikiLink Cell_culture.
- Lipofectamine wikiPageWikiLink Cell_membrane.
- Lipofectamine wikiPageWikiLink Cellular_biology.
- Lipofectamine wikiPageWikiLink DNA.
- Lipofectamine wikiPageWikiLink Gene_delivery.
- Lipofectamine wikiPageWikiLink In_vitro.
- Lipofectamine wikiPageWikiLink Invitrogen.
- Lipofectamine wikiPageWikiLink Life_Technologies_(Thermo_Fisher_Scientific).
- Lipofectamine wikiPageWikiLink Lipofection.
- Lipofectamine wikiPageWikiLink Liposome.
- Lipofectamine wikiPageWikiLink Liposomes.
- Lipofectamine wikiPageWikiLink MRNA.
- Lipofectamine wikiPageWikiLink Messenger_RNA.
- Lipofectamine wikiPageWikiLink Molecular_biology.
- Lipofectamine wikiPageWikiLink Nucleic_acid.
- Lipofectamine wikiPageWikiLink Plasma_membrane.
- Lipofectamine wikiPageWikiLink Plasmid.
- Lipofectamine wikiPageWikiLink RNA.
- Lipofectamine wikiPageWikiLink Reagent.
- Lipofectamine wikiPageWikiLink SiRNA.
- Lipofectamine wikiPageWikiLink Small_interfering_RNA.
- Lipofectamine wikiPageWikiLink Transcription_(genetics).
- Lipofectamine wikiPageWikiLink Transfection.
- Lipofectamine wikiPageWikiLink Transgene.
- Lipofectamine wikiPageWikiLink Vectors_in_gene_therapy.
- Lipofectamine wikiPageWikiLinkText "Lipofectamine".
- Lipofectamine wikiPageWikiLinkText "Lipofectamine®2000".
- Lipofectamine wikiPageWikiLinkText "lipofectamine".
- Lipofectamine hasPhotoCollection Lipofectamine.
- Lipofectamine wikiPageUsesTemplate Template:Reflist.
- Lipofectamine subject Category:Gene_delivery.
- Lipofectamine subject Category:Molecular_biology.
- Lipofectamine hypernym Reagent.
- Lipofectamine type ChemicalCompound.
- Lipofectamine type Technique.
- Lipofectamine comment "Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. Lipofectamine reagent contains lipid subunits that can form liposomes in an aqueous environment, which entrap the transfection materials, i.e. DNA plasmids.".
- Lipofectamine label "Lipofectamine".
- Lipofectamine sameAs m.0285xmd.
- Lipofectamine sameAs Q17139621.
- Lipofectamine sameAs Q17139621.
- Lipofectamine wasDerivedFrom Lipofectamine?oldid=662964953.
- Lipofectamine isPrimaryTopicOf Lipofectamine.