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- LacUV5 abstract "The lacUV5 promoter is a variant of the wild type Escherichia coli lac core promoter. It contains a 2 base pair mutation in the -10 hexamer region, compared to the lac promoter. By conforming closer to the sigma 70 consensus sequence, RNA Polymerase(RNAP) is recruited to the promoter more effectively, even in the absence of additional RNAP interactions via the C-terminal domain (CTD) of the alpha subunit of RNAP. Thus, transcription from lacUV5 will occur at higher rates than seen with Plac. Additionally, unlike Plac, PlacUV5 works independently of activator proteins or other cis regulatory elements (apart from the -10 and -35 promoter regions ). The lacUV5 promoter is commonly found on expression plasmids and is used when controllable but high levels of a product are desired.The lacUV5 promoter expression is regulated by the LacI repressor and can be induced with isopropyl thiogalactoside (IPTG). IPTG is an effective inducer of protein expression when used in the concentration range of 100μM to 1.5mM.The lacUV5 promoter is often found on plasmids used to express protein fusions for further study.".
- LacUV5 wikiPageID "42560314".
- LacUV5 wikiPageLength "2576".
- LacUV5 wikiPageOutDegree "10".
- LacUV5 wikiPageRevisionID "651301182".
- LacUV5 wikiPageWikiLink Activator_(genetics).
- LacUV5 wikiPageWikiLink Category:Escherichia_coli.
- LacUV5 wikiPageWikiLink Category:Gene_expression.
- LacUV5 wikiPageWikiLink Cis-Regulatory_element.
- LacUV5 wikiPageWikiLink Cis-regulatory_element.
- LacUV5 wikiPageWikiLink Consensus_sequence.
- LacUV5 wikiPageWikiLink Escherichia_coli.
- LacUV5 wikiPageWikiLink Lac_operon.
- LacUV5 wikiPageWikiLink Lac_repressor.
- LacUV5 wikiPageWikiLink Plasmid.
- LacUV5 wikiPageWikiLink RNA_polymerase.
- LacUV5 wikiPageWikiLinkText "''lac''UV5".
- LacUV5 hasPhotoCollection LacUV5.
- LacUV5 wikiPageUsesTemplate Template:Multiple_issues.
- LacUV5 subject Category:Escherichia_coli.
- LacUV5 subject Category:Gene_expression.
- LacUV5 hypernym Variant.
- LacUV5 comment "The lacUV5 promoter is a variant of the wild type Escherichia coli lac core promoter. It contains a 2 base pair mutation in the -10 hexamer region, compared to the lac promoter. By conforming closer to the sigma 70 consensus sequence, RNA Polymerase(RNAP) is recruited to the promoter more effectively, even in the absence of additional RNAP interactions via the C-terminal domain (CTD) of the alpha subunit of RNAP. Thus, transcription from lacUV5 will occur at higher rates than seen with Plac.".
- LacUV5 label "LacUV5".
- LacUV5 sameAs m.010fbk1t.
- LacUV5 sameAs Q17141819.
- LacUV5 sameAs Q17141819.
- LacUV5 wasDerivedFrom LacUV5?oldid=651301182.
- LacUV5 isPrimaryTopicOf LacUV5.