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- Job_plot abstract "A Job plot (also known as the method of continuous variation or Job's method; named after P. Job) is used to determine the stoichiometry of a binding event. This method is widely used in analytical chemistry, instrumental analysis, and advanced chemical equilibrium texts and research articles.In solutions where two species are present (i.e. species A and species B), one species (A) may bind to the other species (B). In some cases, more than one A will bind with a single B. One way to determine the amount of A binding to B is by using a Job plot.In this method, the total molar concentration of the two binding partners (e.g. a protein and ligand or a metal and a ligand) are held constant, but their mole fractions are varied. An observable that is proportional to complex formation (such as absorption signal or enzymatic activity) is plotted against the mole fractions of these two components. The maximum (or minimum) on the plot corresponds to the stoichiometry of the two species if sufficiently high concentrations are used. This method is named after P. Job, who introduced this methodology in 1928. An early work of I. Ostromisslensky describes essentially the same approach There are several conditions that must be met in order for Job's method to be applicable:The system must conform to Beer's lawOne complex must predominate under the conditions of the experimentThe Total concentration of the two binding partners must be maintained constantpH and ionic strength must be maintained constant↑ ↑ ↑ ↑".
- Job_plot wikiPageID "13126988".
- Job_plot wikiPageLength "2294".
- Job_plot wikiPageOutDegree "10".
- Job_plot wikiPageRevisionID "613415269".
- Job_plot wikiPageWikiLink Analytical_chemistry.
- Job_plot wikiPageWikiLink Beers_law.
- Job_plot wikiPageWikiLink Beer–Lambert_law.
- Job_plot wikiPageWikiLink Category:Scientific_techniques.
- Job_plot wikiPageWikiLink Chemical_equilibrium.
- Job_plot wikiPageWikiLink Instrumental_analysis.
- Job_plot wikiPageWikiLink Instrumental_chemistry.
- Job_plot wikiPageWikiLink Ligand.
- Job_plot wikiPageWikiLink Molar_concentration.
- Job_plot wikiPageWikiLink Mole_fraction.
- Job_plot wikiPageWikiLink Mole_fractions.
- Job_plot wikiPageWikiLink Protein.
- Job_plot wikiPageWikiLink Stoichiometry.
- Job_plot wikiPageWikiLinkText "Job plot".
- Job_plot hasPhotoCollection Job_plot.
- Job_plot wikiPageUsesTemplate Template:Analytical_chemistry.
- Job_plot wikiPageUsesTemplate Template:Context.
- Job_plot wikiPageUsesTemplate Template:Reflist.
- Job_plot subject Category:Scientific_techniques.
- Job_plot type Article.
- Job_plot type Article.
- Job_plot type Page.
- Job_plot type Technique.
- Job_plot comment "A Job plot (also known as the method of continuous variation or Job's method; named after P. Job) is used to determine the stoichiometry of a binding event. This method is widely used in analytical chemistry, instrumental analysis, and advanced chemical equilibrium texts and research articles.In solutions where two species are present (i.e. species A and species B), one species (A) may bind to the other species (B). In some cases, more than one A will bind with a single B.".
- Job_plot label "Job plot".
- Job_plot sameAs منحنی_جاب.
- Job_plot sameAs m.02z7q8g.
- Job_plot sameAs Q6206742.
- Job_plot sameAs Q6206742.
- Job_plot wasDerivedFrom Job_plot?oldid=613415269.
- Job_plot isPrimaryTopicOf Job_plot.