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- Immunolabeling abstract "Immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. Antigens are organic molecules, usually proteins, capable of binding to an antibody. These antigens can be visualized using a combination of antigen-specific antibody as well as a means of detection, called a tag, that is covalently linked to the antibody. If the immunolabeling process is meant to reveal information about a cell or its substructures, the process is called immunocytochemistry. Immunolabeling of larger structures is called immunohistochemistry.There are two complex steps in the manufacture of antibody for immunolabeling. The first is producing the antibody that binds specifically to the antigen of interest and the second is fusing the tag to the antibody. Since it is impractical to fuse a tag to every conceivable antigen-specific antibody, most immunolabeling processes use an indirect method of detection. This indirect method employs a primary antibody that is antigen-specific and a secondary antibody fused to a tag that specifically binds the primary antibody. This indirect approach permits mass production of secondary antibody that can be bought off the shelf. Pursuant to this indirect method, the primary antibody is added to the test system. The primary antibody seeks out and binds to the target antigen. The tagged secondary antibody, designed to attach exclusively to the primary antibody, is subsequently added.Typical tags include: a fluorescent compound, gold beads, a particular epitope tag, or an enzyme that produces a colored compound. The association of the tags to the target via the antibodies provides for the identification and visualization of the antigen of interest in its native location in the tissue, such as the cell membrane, cytoplasm, or nuclear membrane. Under certain conditions the method can be adapted to provide quantitative information.Immunolabeling can be used in pharmacology, molecular biology, biochemistry and any other field where it is important to know of the precise location of an antibody-bindable molecule.".
- Immunolabeling thumbnail Immunolabeling_process_image.png?width=300.
- Immunolabeling wikiPageExternalLink 899.
- Immunolabeling wikiPageExternalLink g001912.gif.
- Immunolabeling wikiPageExternalLink succinyl.
- Immunolabeling wikiPageExternalLink Vol7_Iss7_Fig2.jpg.
- Immunolabeling wikiPageExternalLink TechIEM.html.
- Immunolabeling wikiPageExternalLink Session%203%20-%20Haigler.pdf.
- Immunolabeling wikiPageExternalLink temtl6.gif.
- Immunolabeling wikiPageID "11503447".
- Immunolabeling wikiPageLength "23169".
- Immunolabeling wikiPageOutDegree "54".
- Immunolabeling wikiPageRevisionID "678927202".
- Immunolabeling wikiPageWikiLink Affinity_chromatography.
- Immunolabeling wikiPageWikiLink Amino_acid.
- Immunolabeling wikiPageWikiLink Antibody.
- Immunolabeling wikiPageWikiLink Antigen.
- Immunolabeling wikiPageWikiLink Antigens.
- Immunolabeling wikiPageWikiLink Biochemistry.
- Immunolabeling wikiPageWikiLink Category:Immunologic_tests.
- Immunolabeling wikiPageWikiLink Category:Medical_diagnosis.
- Immunolabeling wikiPageWikiLink Cell_membrane.
- Immunolabeling wikiPageWikiLink Chromosome.
- Immunolabeling wikiPageWikiLink Condensin.
- Immunolabeling wikiPageWikiLink Cross-reactivity.
- Immunolabeling wikiPageWikiLink Cytoplasm.
- Immunolabeling wikiPageWikiLink Denaturation_(biochemistry).
- Immunolabeling wikiPageWikiLink Electron_Microscopy.
- Immunolabeling wikiPageWikiLink Electron_microscope.
- Immunolabeling wikiPageWikiLink Enzyme.
- Immunolabeling wikiPageWikiLink Epitope.
- Immunolabeling wikiPageWikiLink Fimbria_(bacteriology).
- Immunolabeling wikiPageWikiLink Fluorescence.
- Immunolabeling wikiPageWikiLink Fluorescent.
- Immunolabeling wikiPageWikiLink Fragment_antigen-binding.
- Immunolabeling wikiPageWikiLink Fragment_crystallizable_region.
- Immunolabeling wikiPageWikiLink Hapten.
- Immunolabeling wikiPageWikiLink Immunoblotting.
- Immunolabeling wikiPageWikiLink Immunocytochemistry.
- Immunolabeling wikiPageWikiLink Immunoglobulin_G.
- Immunolabeling wikiPageWikiLink Immunogold_labelling.
- Immunolabeling wikiPageWikiLink Immunohistochemistry.
- Immunolabeling wikiPageWikiLink Immunoprecipitation.
- Immunolabeling wikiPageWikiLink Isotype_(immunology).
- Immunolabeling wikiPageWikiLink Light_microscope.
- Immunolabeling wikiPageWikiLink Microscopy.
- Immunolabeling wikiPageWikiLink Mitosis.
- Immunolabeling wikiPageWikiLink Molecular_biology.
- Immunolabeling wikiPageWikiLink Monoclonal_antibodies.
- Immunolabeling wikiPageWikiLink Monoclonal_antibody.
- Immunolabeling wikiPageWikiLink Nuclear_membrane.
- Immunolabeling wikiPageWikiLink Objective_(optics).
- Immunolabeling wikiPageWikiLink Optical_microscope.
- Immunolabeling wikiPageWikiLink Pharmacology.
- Immunolabeling wikiPageWikiLink Polyclonal_antibodies.
- Immunolabeling wikiPageWikiLink Post-translational_modification.
- Immunolabeling wikiPageWikiLink Posttranslational_modification.
- Immunolabeling wikiPageWikiLink Primary_and_secondary_antibodies.
- Immunolabeling wikiPageWikiLink Protein.
- Immunolabeling wikiPageWikiLink Proteins.
- Immunolabeling wikiPageWikiLink Scanning_Electron_Microscopy.
- Immunolabeling wikiPageWikiLink Scanning_electron_microscope.
- Immunolabeling wikiPageWikiLink TOP2A.
- Immunolabeling wikiPageWikiLink Transmission_Electron_Microscopy.
- Immunolabeling wikiPageWikiLink Transmission_electron_microscope.
- Immunolabeling wikiPageWikiLink Transmission_electron_microscopy.
- Immunolabeling wikiPageWikiLink Western_blot.
- Immunolabeling wikiPageWikiLink File:Immunolabeling_process_image.png.
- Immunolabeling wikiPageWikiLinkText "Immunolabeling".
- Immunolabeling wikiPageWikiLinkText "immunolabeling".
- Immunolabeling wikiPageWikiLinkText "tagged antibody".
- Immunolabeling by "no".
- Immunolabeling hasPhotoCollection Immunolabeling.
- Immunolabeling label "Immunolabelling".
- Immunolabeling onlinebooks "no".
- Immunolabeling wikiPageUsesTemplate Template:Library_resources_box.
- Immunolabeling wikiPageUsesTemplate Template:Reflist.
- Immunolabeling wikititle "Immunolabelling".
- Immunolabeling subject Category:Immunologic_tests.
- Immunolabeling subject Category:Medical_diagnosis.
- Immunolabeling hypernym Process.
- Immunolabeling type Election.
- Immunolabeling comment "Immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. Antigens are organic molecules, usually proteins, capable of binding to an antibody. These antigens can be visualized using a combination of antigen-specific antibody as well as a means of detection, called a tag, that is covalently linked to the antibody.".
- Immunolabeling label "Immunolabeling".
- Immunolabeling sameAs m.02rg1hv.
- Immunolabeling sameAs Q6005532.
- Immunolabeling sameAs Q6005532.
- Immunolabeling wasDerivedFrom Immunolabeling?oldid=678927202.
- Immunolabeling depiction Immunolabeling_process_image.png.
- Immunolabeling isPrimaryTopicOf Immunolabeling.