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- Henderson_limit abstract "The value of the Henderson limit is defined as 2 × 107 Gy (J/kg).Although generalizable, the limit is defined in the context of biomolecular X-ray crystallography, where a typical experiment consists of exposing a single frozen crystal of a macromolecule (generally protein, DNA or RNA) to an intense X-ray beam. The beams that are diffracted are then analyzed towards obtaining an atomically resolved model of the crystal.The limit is defined as the X-ray dose (energy per unit mass) a cryo-cooled crystal can absorb before the diffraction pattern decays to half of its original intensity. Such decay presents itself as a problem for crystallographers who require that the diffraction intensities decay as little as possible, to maximize the signal to noise ratio in order to determine accurate atomic models that describe the crystal.Although the process is still not fully understood, diffraction patterns of crystals typically decay with X-ray exposure due to a number of processes which non uniformly and irreversibly modify molecules that compose the crystal. These modifications induce disorder and thus decrease the intensity of Bragg diffraction. The processes behind these modifications include primary damage via the photo electric effect, covalent modification by free radicals, oxidation (methionine residues), reduction (disulfide bonds) and decarboxylation (glutamate, aspartate residues).".
- Henderson_limit wikiPageID "1943021".
- Henderson_limit wikiPageLength "1574".
- Henderson_limit wikiPageOutDegree "10".
- Henderson_limit wikiPageRevisionID "532035816".
- Henderson_limit wikiPageWikiLink Bragg_diffraction.
- Henderson_limit wikiPageWikiLink Braggs_law.
- Henderson_limit wikiPageWikiLink Category:Molecular_biology.
- Henderson_limit wikiPageWikiLink DNA.
- Henderson_limit wikiPageWikiLink Decarboxylation.
- Henderson_limit wikiPageWikiLink Disulfide.
- Henderson_limit wikiPageWikiLink Disulfide_bond.
- Henderson_limit wikiPageWikiLink Free_radicals.
- Henderson_limit wikiPageWikiLink Photo_electric_effect.
- Henderson_limit wikiPageWikiLink Photoelectric_effect.
- Henderson_limit wikiPageWikiLink Protein.
- Henderson_limit wikiPageWikiLink RNA.
- Henderson_limit wikiPageWikiLink Radical_(chemistry).
- Henderson_limit wikiPageWikiLink X-ray_crystallography.
- Henderson_limit wikiPageWikiLinkText "Henderson limit".
- Henderson_limit hasPhotoCollection Henderson_limit.
- Henderson_limit subject Category:Molecular_biology.
- Henderson_limit comment "The value of the Henderson limit is defined as 2 × 107 Gy (J/kg).Although generalizable, the limit is defined in the context of biomolecular X-ray crystallography, where a typical experiment consists of exposing a single frozen crystal of a macromolecule (generally protein, DNA or RNA) to an intense X-ray beam.".
- Henderson_limit label "Henderson limit".
- Henderson_limit sameAs Henderson-Grenze.
- Henderson_limit sameAs m.067w45.
- Henderson_limit sameAs Q5713467.
- Henderson_limit sameAs Q5713467.
- Henderson_limit wasDerivedFrom Henderson_limit?oldid=532035816.
- Henderson_limit isPrimaryTopicOf Henderson_limit.