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- Gel_electrophoresis_of_nucleic_acids abstract "Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in "nucleotides", "base pairs" or "kb" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a "slab" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.".
- Gel_electrophoresis_of_nucleic_acids thumbnail DNA_Agarose_Gel_Electrophor.jpg?width=300.
- Gel_electrophoresis_of_nucleic_acids wikiPageID "238301".
- Gel_electrophoresis_of_nucleic_acids wikiPageLength "23050".
- Gel_electrophoresis_of_nucleic_acids wikiPageOutDegree "63".
- Gel_electrophoresis_of_nucleic_acids wikiPageRevisionID "681639827".
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Agarose_gel_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Alkaline_gel_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Anode.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Biotium.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Bromophenol_blue.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Capillary_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Category:DNA.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Category:Electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Category:Molecular_biology.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Chemical_structure.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Cresol_Red.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Cross-link.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink DNA.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink DNA_damage.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink DNA_repair.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink DNA_sequencing.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink DNA_supercoil.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Electric_field.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Electropherogram.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Electrophoretic_mobility_shift_assay.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Ethidium_bromide.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:Electropherogram_trace.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Fluorescence.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Fluorescent_dye.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Fluorophore.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Gel.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink GelGreen.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink GelRed.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Hybridization_probe.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink ImageJ.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Invitrogen.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Logarithm.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Molecular-weight_size_marker.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Molecular_weight_size_marker.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Mutagen.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Orange_G.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink PAGE.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink PCR.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Phosphate.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Polyacrylamide_gel.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Polymerase_chain_reaction.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Pulse_field_gel_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Pulsed-field_gel_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Pulsed_field_gel_electrophoresis.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink RNA.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Random_coil.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Reptation.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Restriction_digest.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Restriction_endonuclease.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Restriction_enzyme.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink SDS-PAGE.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink SYBR_Green.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink SYBR_Green_I.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink SYBR_Safe.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Southern_blot.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Supercoiled_DNA.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Ultraviolet.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink Xylene_cyanol.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:Agarosegel.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:AgarosegelUV.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:Agarosegelphoto.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:DNA_Agarose_Gel_Electrophor.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:Gel_electrophoresis_2.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLink File:Plasmid_miniprep.jpg.
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLinkText "Effect of circular DNA on gel electrophoresis".
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLinkText "Electrophoretic separation".
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLinkText "Gel electrophoresis of nucleic acids".
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLinkText "Gel electrophoresis".
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLinkText "digital electrophoresis gel image".
- Gel_electrophoresis_of_nucleic_acids wikiPageWikiLinkText "gel electrophoresis of nucleic acids".
- Gel_electrophoresis_of_nucleic_acids hasPhotoCollection Gel_electrophoresis_of_nucleic_acids.
- Gel_electrophoresis_of_nucleic_acids wikiPageUsesTemplate Template:Reflist.
- Gel_electrophoresis_of_nucleic_acids subject Category:DNA.
- Gel_electrophoresis_of_nucleic_acids subject Category:Electrophoresis.
- Gel_electrophoresis_of_nucleic_acids subject Category:Molecular_biology.
- Gel_electrophoresis_of_nucleic_acids hypernym Technique.
- Gel_electrophoresis_of_nucleic_acids type Software.
- Gel_electrophoresis_of_nucleic_acids type Method.
- Gel_electrophoresis_of_nucleic_acids type Process.
- Gel_electrophoresis_of_nucleic_acids type Technique.
- Gel_electrophoresis_of_nucleic_acids comment "Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain.".
- Gel_electrophoresis_of_nucleic_acids label "Gel electrophoresis of nucleic acids".
- Gel_electrophoresis_of_nucleic_acids sameAs m.01jklq.
- Gel_electrophoresis_of_nucleic_acids sameAs Электрофорез_ДНК.
- Gel_electrophoresis_of_nucleic_acids sameAs Elektroforeza_DNK.
- Gel_electrophoresis_of_nucleic_acids sameAs Q4531118.
- Gel_electrophoresis_of_nucleic_acids sameAs Q4531118.
- Gel_electrophoresis_of_nucleic_acids wasDerivedFrom Gel_electrophoresis_of_nucleic_acids?oldid=681639827.
- Gel_electrophoresis_of_nucleic_acids depiction DNA_Agarose_Gel_Electrophor.jpg.
- Gel_electrophoresis_of_nucleic_acids isPrimaryTopicOf Gel_electrophoresis_of_nucleic_acids.