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• Flow_cytometry_bioinformatics abstract "Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing flow cytometry data using extensive computational resources and tools.Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning.Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results.Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. For preprocessing, this includes compensating for spectral overlap, transforming data onto scales conducive to visualization and analysis, assessing data for quality, and normalizing data across samples and experiments.For population identification, tools are available to aid traditional manual identification of populations in two-dimensional scatter plots (gating), to use dimensionality reduction to aid gating, and to find populations automatically in higher-dimensional space in a variety of ways.It is also possible to characterize data in more comprehensive ways, such as the density-guided binary space partitioning technique known as probability binning, or by combinatorial gating.Finally, diagnosis using flow cytometry data can be aided by supervised learning techniques, and discovery of new cell types of biological importance by high-throughput statistical methods, as part of pipelines incorporating all of the aforementioned methods.Open standards, data and software are also key parts of flow cytometry bioinformatics.Data standards include the widely adopted Flow Cytometry Standard (FCS) defining how data from cytometers should be stored, but also several new standards under development by the International Society for Advancement of Cytometry (ISAC) to aid in storing more detailed information about experimental design and analytical steps.Open data is slowly growing with the opening of the CytoBank database in 2010, and FlowRepository in 2012, both of which allow users to freely distribute their data, and the latter of which has been recommended as the preferred repository for MIFlowCyt-compliant data by ISAC.Open software is most widely available in the form of a suite of Bioconductor packages, but is also available for web execution on the GenePattern platform.".
• Flow_cytometry_bioinformatics thumbnail Cytometer.svg?width=300.
• Flow_cytometry_bioinformatics wikiPageExternalLink www.facsanadu.org.
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• Flow_cytometry_bioinformatics wikiPageRevisionID "679070420".
• Flow_cytometry_bioinformatics wikiPageWikiLink Antibody.
• Flow_cytometry_bioinformatics wikiPageWikiLink Autofluorescence.
• Flow_cytometry_bioinformatics wikiPageWikiLink Binary_space_partitioning.
• Flow_cytometry_bioinformatics wikiPageWikiLink Bioconductor.
• Flow_cytometry_bioinformatics wikiPageWikiLink Bioinformatics.
• Flow_cytometry_bioinformatics wikiPageWikiLink Biomarker_(cell).
• Flow_cytometry_bioinformatics wikiPageWikiLink CD4.
• Flow_cytometry_bioinformatics wikiPageWikiLink Cancer.
• Flow_cytometry_bioinformatics wikiPageWikiLink Category:Bioinformatics.
• Flow_cytometry_bioinformatics wikiPageWikiLink Category:Flow_cytometry.
• Flow_cytometry_bioinformatics wikiPageWikiLink Cell_(biology).
• Flow_cytometry_bioinformatics wikiPageWikiLink Chlorophyll.
• Flow_cytometry_bioinformatics wikiPageWikiLink Cloud_computing.
• Flow_cytometry_bioinformatics wikiPageWikiLink Cluster_analysis.
• Flow_cytometry_bioinformatics wikiPageWikiLink Comma-separated_values.
• Flow_cytometry_bioinformatics wikiPageWikiLink Computational_statistics.
• Flow_cytometry_bioinformatics wikiPageWikiLink Covalent_bond.
• Flow_cytometry_bioinformatics wikiPageWikiLink DNA.
• Flow_cytometry_bioinformatics wikiPageWikiLink Data_transformation_(statistics).
• Flow_cytometry_bioinformatics wikiPageWikiLink Dimension.
• Flow_cytometry_bioinformatics wikiPageWikiLink Dimension_reduction.
• Flow_cytometry_bioinformatics wikiPageWikiLink Dimensionality_reduction.
• Flow_cytometry_bioinformatics wikiPageWikiLink Emission_spectrum.
• Flow_cytometry_bioinformatics wikiPageWikiLink Empirical_distribution_function.
• Flow_cytometry_bioinformatics wikiPageWikiLink File_format.
• Flow_cytometry_bioinformatics wikiPageWikiLink Filter_(optics).
• Flow_cytometry_bioinformatics wikiPageWikiLink Flow_Cytometry_Standard.
• Flow_cytometry_bioinformatics wikiPageWikiLink Flow_cytometry.
• Flow_cytometry_bioinformatics wikiPageWikiLink Fluorescence.
• Flow_cytometry_bioinformatics wikiPageWikiLink Fluorophore.
• Flow_cytometry_bioinformatics wikiPageWikiLink Free_and_open-source_software.
• Flow_cytometry_bioinformatics wikiPageWikiLink Free_and_open_source_software.
• Flow_cytometry_bioinformatics wikiPageWikiLink Gate_(cytometry).
• Flow_cytometry_bioinformatics wikiPageWikiLink GenePattern.
• Flow_cytometry_bioinformatics wikiPageWikiLink Gene_expression.
• Flow_cytometry_bioinformatics wikiPageWikiLink Genetic_variation.
• Flow_cytometry_bioinformatics wikiPageWikiLink Green_fluorescent_protein.
• Flow_cytometry_bioinformatics wikiPageWikiLink Grubbs_test_for_outliers.
• Flow_cytometry_bioinformatics wikiPageWikiLink HIV.
• Flow_cytometry_bioinformatics wikiPageWikiLink Histogram.
• Flow_cytometry_bioinformatics wikiPageWikiLink Hybridization_probe.
• Flow_cytometry_bioinformatics wikiPageWikiLink Hydrodynamic_focusing.
• Flow_cytometry_bioinformatics wikiPageWikiLink Image_registration.
• Flow_cytometry_bioinformatics wikiPageWikiLink Information_visualization.
• Flow_cytometry_bioinformatics wikiPageWikiLink Intensity_(physics).
• Flow_cytometry_bioinformatics wikiPageWikiLink Inverse_hyperbolic_function.
• Flow_cytometry_bioinformatics wikiPageWikiLink Invertible_matrix.
• Flow_cytometry_bioinformatics wikiPageWikiLink Kernel_density_estimate.
• Flow_cytometry_bioinformatics wikiPageWikiLink Kernel_density_estimation.
• Flow_cytometry_bioinformatics wikiPageWikiLink Kolmogorov–Smirnov_test.
• Flow_cytometry_bioinformatics wikiPageWikiLink Light_scattering.
• Flow_cytometry_bioinformatics wikiPageWikiLink Linear_equation.
• Flow_cytometry_bioinformatics wikiPageWikiLink Log-normal_distribution.
• Flow_cytometry_bioinformatics wikiPageWikiLink Log_amplifier.
• Flow_cytometry_bioinformatics wikiPageWikiLink Logarithmic_scale.
• Flow_cytometry_bioinformatics wikiPageWikiLink Machine_learning.
• Flow_cytometry_bioinformatics wikiPageWikiLink Metabolite.
• Flow_cytometry_bioinformatics wikiPageWikiLink Microbiome.
• Flow_cytometry_bioinformatics wikiPageWikiLink Microbiota.
• Flow_cytometry_bioinformatics wikiPageWikiLink Microfluidics.
• Flow_cytometry_bioinformatics wikiPageWikiLink Minimum_spanning_tree.
• Flow_cytometry_bioinformatics wikiPageWikiLink National_Institutes_of_Health.
• Flow_cytometry_bioinformatics wikiPageWikiLink Nature_Publishing_Group.
• Flow_cytometry_bioinformatics wikiPageWikiLink NetCDF.
• Flow_cytometry_bioinformatics wikiPageWikiLink Normalization_(statistics).
• Flow_cytometry_bioinformatics wikiPageWikiLink Open-source_software.
• Flow_cytometry_bioinformatics wikiPageWikiLink Open_data.
• Flow_cytometry_bioinformatics wikiPageWikiLink Open_science_data.
• Flow_cytometry_bioinformatics wikiPageWikiLink Open_standard.
• Flow_cytometry_bioinformatics wikiPageWikiLink Optical_filter.
• Flow_cytometry_bioinformatics wikiPageWikiLink Pacific_Biosciences.
• Flow_cytometry_bioinformatics wikiPageWikiLink Photomultiplier.
• Flow_cytometry_bioinformatics wikiPageWikiLink Power_transform.
• Flow_cytometry_bioinformatics wikiPageWikiLink Principal_component_analysis.
• Flow_cytometry_bioinformatics wikiPageWikiLink Protein.
• Flow_cytometry_bioinformatics wikiPageWikiLink Proteomics.
• Flow_cytometry_bioinformatics wikiPageWikiLink Quantile.
• Flow_cytometry_bioinformatics wikiPageWikiLink RNA.
• Flow_cytometry_bioinformatics wikiPageWikiLink R_(programming_language).
• Flow_cytometry_bioinformatics wikiPageWikiLink Rare_earth_element.
• Flow_cytometry_bioinformatics wikiPageWikiLink Real-time_polymerase_chain_reaction.
• Flow_cytometry_bioinformatics wikiPageWikiLink Scatter_plot.
• Flow_cytometry_bioinformatics wikiPageWikiLink Scientific_workflow_system.
• Flow_cytometry_bioinformatics wikiPageWikiLink Semi-supervised_learning.
• Flow_cytometry_bioinformatics wikiPageWikiLink Sperm_sorting.
• Flow_cytometry_bioinformatics wikiPageWikiLink Standard_deviation.
• Flow_cytometry_bioinformatics wikiPageWikiLink Stanford_University.
• Flow_cytometry_bioinformatics wikiPageWikiLink Supervised_learning.
• Flow_cytometry_bioinformatics wikiPageWikiLink Telomere.
• Flow_cytometry_bioinformatics wikiPageWikiLink Time-of-flight_mass_spectrometry.
• Flow_cytometry_bioinformatics wikiPageWikiLink Transgenesis.
• Flow_cytometry_bioinformatics wikiPageWikiLink Workflow.
• Flow_cytometry_bioinformatics wikiPageWikiLink World_Wide_Web_Consortium.

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