Matches in DBpedia 2015-10 for { <http://dbpedia.org/resource/ELISA> ?p ?o }
- ELISA abstract "The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance.ELISA is a popular format of "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Of note, ELISA can perform other forms of ligand binding assays instead of strictly "immuno" assays, though the name carried the original "immuno" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or "immunosorbed" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, and so are not easily reusable.".
- ELISA thumbnail Microtiter_plate.JPG?width=300.
- ELISA wikiPageExternalLink main.html.
- ELISA wikiPageExternalLink ZWxpc2Ega2l0cw==.html.
- ELISA wikiPageExternalLink elisa-animation.html.
- ELISA wikiPageExternalLink elisaformats.
- ELISA wikiPageExternalLink ELISA.html.
- ELISA wikiPageExternalLink kb07an01.htm.
- ELISA wikiPageExternalLink elisa-assay-principle.
- ELISA wikiPageID "95162".
- ELISA wikiPageLength "25110".
- ELISA wikiPageOutDegree "80".
- ELISA wikiPageRevisionID "679518163".
- ELISA wikiPageWikiLink 3,3,5,5-Tetramethylbenzidine.
- ELISA wikiPageWikiLink 3,3’,5,5’-tetramethylbenzidine.
- ELISA wikiPageWikiLink ABTS.
- ELISA wikiPageWikiLink Adsorption.
- ELISA wikiPageWikiLink Almond.
- ELISA wikiPageWikiLink Antibody.
- ELISA wikiPageWikiLink Antigen.
- ELISA wikiPageWikiLink Antigen-antibody_interaction.
- ELISA wikiPageWikiLink Assay.
- ELISA wikiPageWikiLink Attogram.
- ELISA wikiPageWikiLink Biochemistry.
- ELISA wikiPageWikiLink Bioconjugation.
- ELISA wikiPageWikiLink Blood_plasma.
- ELISA wikiPageWikiLink Bovine_serum_albumin.
- ELISA wikiPageWikiLink Casein.
- ELISA wikiPageWikiLink Category:Immunologic_tests.
- ELISA wikiPageWikiLink Chagas_disease.
- ELISA wikiPageWikiLink Chromogenic.
- ELISA wikiPageWikiLink Coeliac_disease.
- ELISA wikiPageWikiLink Detergent.
- ELISA wikiPageWikiLink AIDS.
- ELISA wikiPageWikiLink Egg_(food).
- ELISA wikiPageWikiLink Electrochemiluminescence.
- ELISA wikiPageWikiLink Enterotoxin.
- ELISA wikiPageWikiLink Enzyme.
- ELISA wikiPageWikiLink Enzyme_substrate.
- ELISA wikiPageWikiLink Eva_Engvall.
- ELISA wikiPageWikiLink Fluorescence.
- ELISA wikiPageWikiLink Fluorogenic.
- ELISA wikiPageWikiLink Food.
- ELISA wikiPageWikiLink Food_allergy.
- ELISA wikiPageWikiLink HIV_test.
- ELISA wikiPageWikiLink Horseradish_peroxidase.
- ELISA wikiPageWikiLink Immunoassay.
- ELISA wikiPageWikiLink Immunoscreening.
- ELISA wikiPageWikiLink In_vitro_diagnostics.
- ELISA wikiPageWikiLink Jerker_Porath.
- ELISA wikiPageWikiLink Lateral_flow_test.
- ELISA wikiPageWikiLink Ligand_binding_assay.
- ELISA wikiPageWikiLink Ligand_binding_assays.
- ELISA wikiPageWikiLink Magnetic_immunoassay.
- ELISA wikiPageWikiLink Malaria.
- ELISA wikiPageWikiLink Medical_diagnosis.
- ELISA wikiPageWikiLink Medical_laboratories.
- ELISA wikiPageWikiLink Medical_laboratory.
- ELISA wikiPageWikiLink Medical_test.
- ELISA wikiPageWikiLink Microtiter_plate.
- ELISA wikiPageWikiLink Milk.
- ELISA wikiPageWikiLink Multiplex_(assay).
- ELISA wikiPageWikiLink Mycobacterium.
- ELISA wikiPageWikiLink Orders_of_magnitude_(mass).
- ELISA wikiPageWikiLink Paratuberculosis.
- ELISA wikiPageWikiLink Peanut.
- ELISA wikiPageWikiLink Plant_pathology.
- ELISA wikiPageWikiLink Plaque_reduction_neutralization_test.
- ELISA wikiPageWikiLink Plate_reader.
- ELISA wikiPageWikiLink Polystyrene.
- ELISA wikiPageWikiLink Primary_and_secondary_antibodies.
- ELISA wikiPageWikiLink Primary_antibody.
- ELISA wikiPageWikiLink Quality_control.
- ELISA wikiPageWikiLink Quantitative_PCR.
- ELISA wikiPageWikiLink Radioactive.
- ELISA wikiPageWikiLink Radioactive_decay.
- ELISA wikiPageWikiLink Radioimmunoassay.
- ELISA wikiPageWikiLink Real-time_polymerase_chain_reaction.
- ELISA wikiPageWikiLink Reflectometry.
- ELISA wikiPageWikiLink Rosalyn_Sussman_Yalow.
- ELISA wikiPageWikiLink Secondary_antibody.
- ELISA wikiPageWikiLink Secretion_assay.
- ELISA wikiPageWikiLink Sensitivity_and_specificity.
- ELISA wikiPageWikiLink Signal_(electrical_engineering).
- ELISA wikiPageWikiLink Signal_(information_theory).
- ELISA wikiPageWikiLink Solomon_Berson.
- ELISA wikiPageWikiLink Spectrophotometry.
- ELISA wikiPageWikiLink Substrate_(biochemistry).
- ELISA wikiPageWikiLink Substrate_(chemistry).
- ELISA wikiPageWikiLink University_of_Arizona.
- ELISA wikiPageWikiLink Walnut.
- ELISA wikiPageWikiLink West_Nile_virus.
- ELISA wikiPageWikiLink File:ELISA-sandwich.svg.
- ELISA wikiPageWikiLink File:ELISA.jpg.
- ELISA wikiPageWikiLink File:ELISA_diagram.png.
- ELISA wikiPageWikiLink File:Elisa10.jpg.
- ELISA wikiPageWikiLink File:Pn-lab-2001nov.jpg.
- ELISA wikiPageWikiLinkText "AIDS antibody testing".
- ELISA wikiPageWikiLinkText "EIA".
- ELISA wikiPageWikiLinkText "ELISA Inhibition ASTM D6499-07 test".