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- Direct_fluorescent_antibody abstract "A direct fluorescent antibody (DFA or dFA) (also known as "Direct immunofluorescence") is an antibody that has been tagged in a direct fluorescent antibody test. Its name derives from the fact that it directly tests the presence of an antigen with the tagged antibody, unlike western blotting, which uses an indirect method of detection, where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached to the secondary antibody. Commercial DFA testing kits are available, which contain fluorescently labelled antibodies, designed to specifically target unique antigens present in the bacteria or virus, but not present in mammals (Eukaryotes). This technique can be used to quickly determine if a subject has a specific viral or bacterial infection. In the case of respiratory viruses, many of which have similar broad symptoms, detection can be carried out using nasal wash samples from the subject with the suspected infection. Although shedding cells in the respiratory tract can be obtained, it is often in low numbers, and so an alternative method can be adopted where compatible cell culture can be exposed to infected nasal wash samples, so if the virus is present it can be grown up to a larger quantity, which can then give a clearer positive or negative reading. As with all types of fluorescence microscopy, the correct absorption wavelength needs to be determined in order to excite the fluorophore tag attached to the antibody, and detect the fluorescence given off, which indicates which cells are positive for the presence of the virus or bacteria being detected. Direct immunofluorescence can be used to detect deposits of immunoglobulins and complement proteins in biopsies of skin, kidney and other organs. Their presence is indicative of an autoimmune disease. When skin not exposed to the sun is tested, a positive direct IF (the so-called Lupus band test) is an evidence of systemic lupus erythematosus. Direct fluorescent antibody can also be used to detect parasitic infections, as was pioneered by Sadun, et al. (1960).".
- Direct_fluorescent_antibody thumbnail Chlamydophila_psittaci_FA_stain.jpg?width=300.
- Direct_fluorescent_antibody wikiPageID "2206783".
- Direct_fluorescent_antibody wikiPageLength "3335".
- Direct_fluorescent_antibody wikiPageOutDegree "20".
- Direct_fluorescent_antibody wikiPageRevisionID "660241943".
- Direct_fluorescent_antibody wikiPageWikiLink Absorption_band.
- Direct_fluorescent_antibody wikiPageWikiLink Antibody.
- Direct_fluorescent_antibody wikiPageWikiLink Antigen.
- Direct_fluorescent_antibody wikiPageWikiLink Autoimmune_disease.
- Direct_fluorescent_antibody wikiPageWikiLink Category:Clinical_pathology.
- Direct_fluorescent_antibody wikiPageWikiLink Category:Immunologic_tests.
- Direct_fluorescent_antibody wikiPageWikiLink Category:Laboratory_techniques.
- Direct_fluorescent_antibody wikiPageWikiLink Complementarity_(molecular_biology).
- Direct_fluorescent_antibody wikiPageWikiLink Elvio_Sadun.
- Direct_fluorescent_antibody wikiPageWikiLink Eukaryote.
- Direct_fluorescent_antibody wikiPageWikiLink Eukaryotes.
- Direct_fluorescent_antibody wikiPageWikiLink Fluorescence_microscope.
- Direct_fluorescent_antibody wikiPageWikiLink Fluorescence_microscopy.
- Direct_fluorescent_antibody wikiPageWikiLink Fluorophore.
- Direct_fluorescent_antibody wikiPageWikiLink Immunofluorescence.
- Direct_fluorescent_antibody wikiPageWikiLink Immunoglobulins.
- Direct_fluorescent_antibody wikiPageWikiLink Immunolabeling.
- Direct_fluorescent_antibody wikiPageWikiLink Indirect_antibody.
- Direct_fluorescent_antibody wikiPageWikiLink Lupus_band_test.
- Direct_fluorescent_antibody wikiPageWikiLink Primary_and_secondary_antibodies.
- Direct_fluorescent_antibody wikiPageWikiLink Systemic_lupus_erythematosus.
- Direct_fluorescent_antibody wikiPageWikiLink Western_blot.
- Direct_fluorescent_antibody wikiPageWikiLink Western_blotting.
- Direct_fluorescent_antibody wikiPageWikiLink File:Chlamydophila_psittaci_FA_stain.jpg.
- Direct_fluorescent_antibody wikiPageWikiLinkText "DFA".
- Direct_fluorescent_antibody wikiPageWikiLinkText "Direct fluorescent antibody".
- Direct_fluorescent_antibody wikiPageWikiLinkText "direct fluorescent antibody detection (DFA)".
- Direct_fluorescent_antibody wikiPageWikiLinkText "direct fluorescent antibody".
- Direct_fluorescent_antibody wikiPageWikiLinkText "direct immunofluorescence".
- Direct_fluorescent_antibody wikiPageWikiLinkText "direct".
- Direct_fluorescent_antibody wikiPageWikiLinkText "fluorescent antibodies".
- Direct_fluorescent_antibody wikiPageWikiLinkText "fluorescent antibody imaging".
- Direct_fluorescent_antibody wikiPageWikiLinkText "fluorescent antibody testing".
- Direct_fluorescent_antibody wikiPageWikiLinkText "fluorescent antibody tests".
- Direct_fluorescent_antibody wikiPageWikiLinkText "fluorescent antibody".
- Direct_fluorescent_antibody hasPhotoCollection Direct_fluorescent_antibody.
- Direct_fluorescent_antibody wikiPageUsesTemplate Template:Commons_category.
- Direct_fluorescent_antibody wikiPageUsesTemplate Template:Immunologic_techniques_and_tests.
- Direct_fluorescent_antibody wikiPageUsesTemplate Template:MeshName.
- Direct_fluorescent_antibody wikiPageUsesTemplate Template:Refimprove.
- Direct_fluorescent_antibody wikiPageUsesTemplate Template:Reflist.
- Direct_fluorescent_antibody subject Category:Clinical_pathology.
- Direct_fluorescent_antibody subject Category:Immunologic_tests.
- Direct_fluorescent_antibody subject Category:Laboratory_techniques.
- Direct_fluorescent_antibody hypernym Antibody.
- Direct_fluorescent_antibody type Article.
- Direct_fluorescent_antibody type Drug.
- Direct_fluorescent_antibody type Article.
- Direct_fluorescent_antibody type Laboratory.
- Direct_fluorescent_antibody type Technique.
- Direct_fluorescent_antibody comment "A direct fluorescent antibody (DFA or dFA) (also known as "Direct immunofluorescence") is an antibody that has been tagged in a direct fluorescent antibody test. Its name derives from the fact that it directly tests the presence of an antigen with the tagged antibody, unlike western blotting, which uses an indirect method of detection, where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached to the secondary antibody.".
- Direct_fluorescent_antibody label "Direct fluorescent antibody".
- Direct_fluorescent_antibody sameAs پادتن_فلورسانت.
- Direct_fluorescent_antibody sameAs m.06vxz8.
- Direct_fluorescent_antibody sameAs อิมมูโนฟลูออเรสเซนต์โดยตรง.
- Direct_fluorescent_antibody sameAs Q5280334.
- Direct_fluorescent_antibody sameAs Q5280334.
- Direct_fluorescent_antibody wasDerivedFrom Direct_fluorescent_antibody?oldid=660241943.
- Direct_fluorescent_antibody depiction Chlamydophila_psittaci_FA_stain.jpg.
- Direct_fluorescent_antibody isPrimaryTopicOf Direct_fluorescent_antibody.