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- Diethylpyrocarbonate abstract "Diethylpyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues.DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases.Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC. Inactivation of DEPC in this manner yields CO2 and ethanol. Higher concentrations of DEPC are capable of deactivating larger amounts of RNase, but remaining traces or byproducts may inhibit further biochemical reactions such as in vitro translation. Furthermore, chemical modification of RNA such as carboxymethylation is possible when traces of DEPC or its byproducts are present, resulting in impaired recovery of intact RNA even after buffer exchange (after precipitation).DEPC is unstable in water and susceptible to hydrolysis to carbon dioxide and ethanol, especially in the presence of a nucleophile. For this reason, DEPC cannot be used with Tris or HEPES buffers. In contrast, it can be used with phosphate-buffered saline or MOPS. A handy rule is that enzymes or chemicals which have active -O:, -N: or -S: cannot be treated with DEPC to become RNase-free, as DEPC reacts with these species. Furthermore, DEPC degradation products can inhibit in vitro transcription.DEPC derivatization of histidines is also used to study the importance of histidyl residues in enzymes. Modification of histidine by DEPC results in carbethoxylated derivates at the N-omega-2 nitrogen of the imidazole ring. DEPC modification of histidines can be reversed by treatment with 0.5 M hydroxylamine at neutral pH.DEPC can also be used for probing the structure of double-stranded DNA.".
- Diethylpyrocarbonate iupacName "Diethyl dicarbonate".
- Diethylpyrocarbonate thumbnail Diethyl_pyrocarbonate.svg?width=300.
- Diethylpyrocarbonate wikiPageExternalLink DEPC.
- Diethylpyrocarbonate wikiPageExternalLink tb_178.html.
- Diethylpyrocarbonate wikiPageID "1426019".
- Diethylpyrocarbonate wikiPageLength "5065".
- Diethylpyrocarbonate wikiPageOutDegree "25".
- Diethylpyrocarbonate wikiPageRevisionID "671493334".
- Diethylpyrocarbonate wikiPageWikiLink Autoclave.
- Diethylpyrocarbonate wikiPageWikiLink Category:Carbonate_esters.
- Diethylpyrocarbonate wikiPageWikiLink Covalent_bond.
- Diethylpyrocarbonate wikiPageWikiLink Cysteine.
- Diethylpyrocarbonate wikiPageWikiLink Derivatization.
- Diethylpyrocarbonate wikiPageWikiLink Di-tert-butyl_dicarbonate.
- Diethylpyrocarbonate wikiPageWikiLink Dimethyl_dicarbonate.
- Diethylpyrocarbonate wikiPageWikiLink Enzyme.
- Diethylpyrocarbonate wikiPageWikiLink Ethanol.
- Diethylpyrocarbonate wikiPageWikiLink HEPES.
- Diethylpyrocarbonate wikiPageWikiLink Histidine.
- Diethylpyrocarbonate wikiPageWikiLink Hydroxylamine.
- Diethylpyrocarbonate wikiPageWikiLink IUPAC.
- Diethylpyrocarbonate wikiPageWikiLink Imidazole.
- Diethylpyrocarbonate wikiPageWikiLink International_Union_of_Pure_and_Applied_Chemistry.
- Diethylpyrocarbonate wikiPageWikiLink Laboratory.
- Diethylpyrocarbonate wikiPageWikiLink Lysine.
- Diethylpyrocarbonate wikiPageWikiLink MOPS.
- Diethylpyrocarbonate wikiPageWikiLink Nucleophile.
- Diethylpyrocarbonate wikiPageWikiLink Phosphate-buffered_saline.
- Diethylpyrocarbonate wikiPageWikiLink RNA.
- Diethylpyrocarbonate wikiPageWikiLink RNase.
- Diethylpyrocarbonate wikiPageWikiLink Ribonuclease.
- Diethylpyrocarbonate wikiPageWikiLink Tris.
- Diethylpyrocarbonate wikiPageWikiLink Tyrosine.
- Diethylpyrocarbonate wikiPageWikiLink File:Depc_treated_water.jpg.
- Diethylpyrocarbonate wikiPageWikiLinkText "Diethylpyrocarbonate".
- Diethylpyrocarbonate wikiPageWikiLinkText "diethylpyrocarbonate".
- Diethylpyrocarbonate hasPhotoCollection Diethylpyrocarbonate.
- Diethylpyrocarbonate imagefile "Diethyl pyrocarbonate.svg".
- Diethylpyrocarbonate imagefile "Diethyl pyrocarbonate3d.png".
- Diethylpyrocarbonate iupacname "Diethyl dicarbonate".
- Diethylpyrocarbonate othernames "Diethyl oxydiformateEthoxyformic anhydridePyrocarbonic acid diethyl esterDEPC".
- Diethylpyrocarbonate verifiedrevid "443637574".
- Diethylpyrocarbonate watchedfields "changed".
- Diethylpyrocarbonate wikiPageUsesTemplate Template:Chembox.
- Diethylpyrocarbonate wikiPageUsesTemplate Template:Reflist.
- Diethylpyrocarbonate subject Category:Carbonate_esters.
- Diethylpyrocarbonate type ChemicalCompound.
- Diethylpyrocarbonate type ChemicalSubstance.
- Diethylpyrocarbonate type ChemicalObject.
- Diethylpyrocarbonate type Thing.
- Diethylpyrocarbonate type Q11173.
- Diethylpyrocarbonate comment "Diethylpyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils.".
- Diethylpyrocarbonate label "Diethylpyrocarbonate".
- Diethylpyrocarbonate sameAs Diethyldicarbonat.
- Diethylpyrocarbonate sameAs دیاتیلپیروکربنات.
- Diethylpyrocarbonate sameAs Pyrocarbonate_dxc3xa9thyle.
- Diethylpyrocarbonate sameAs Dietilpirocarbonato.
- Diethylpyrocarbonate sameAs ジエチルピロカーボネート.
- Diethylpyrocarbonate sameAs Dicarbonato_de_dietila.
- Diethylpyrocarbonate sameAs m.050krb.
- Diethylpyrocarbonate sameAs Диэтилпирокарбонат.
- Diethylpyrocarbonate sameAs Dietilpirokarbonat.
- Diethylpyrocarbonate sameAs Dietilpirokarbonat.
- Diethylpyrocarbonate sameAs Диетилпірокарбонат.
- Diethylpyrocarbonate sameAs Q421385.
- Diethylpyrocarbonate sameAs Q421385.
- Diethylpyrocarbonate wasDerivedFrom Diethylpyrocarbonate?oldid=671493334.
- Diethylpyrocarbonate depiction Diethyl_pyrocarbonate.svg.
- Diethylpyrocarbonate isPrimaryTopicOf Diethylpyrocarbonate.