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- Deletion_mapping abstract "In genetics and especially genetic engineering, deletion mapping is a technique used to ascertain the location of mutation sites within a gene. The principle of deletion mapping involves crossing a strain which has a point mutation in a gene, with multiple strains who each carry a deletion in a different region of the same gene. Wherever recombination occurs between the two strains to produce a wild-type (+) gene (regardless of frequency), the point mutation cannot lie within the region of the deletion. If recombination cannot produce any wild-type genes, then it is reasonable to conclude that the point mutation and deletion are found within the same stretch of DNA.This example should demonstrate how the principle works:Suppose you have a gene X, which in wild-type (+) form can be shown linearly like so:5'-------------------------------------------------------------------------------------------------------------------3' gene X, +Suppose a strain of organisms has a point mutation in the gene (now called gene X, – to denote that it is no longer wild-type):5'----------------------------------------------------X--------------------------------------------------------------3' gene X, –Now suppose you have two strains of organisms, each with deletions in gene X at different sites, called del-1 and del-2, respectively (the dotted line indicates the site of deletion):5'-------------------(..............................................)------------------------------------------------3' del-15'------------------------------------------------------------(............................................)---------3' del-2Because the point mutation lies within the deletion of del-1, there will be no wild-type (+) recombinants between the point mutant and the del-1 mutant. However, in a cross between the point mutant and the del-2 mutant, there could be a successful wild-type (+) recombinant produced.In genetic recombination, if a mutant allele in the donor is within the sequence corresponding to the region deleted in the recipient, then no (+) recombinants will be obtained (as in the cross with del-1). To repair a deletion by recombination, the donor must have wild-type DNA sequence in the region corresponding to the DNA deleted in the recipient (as in the cross against del-2). In other words, there is a feasible recombination possibility between the point mutant and del-2 in which a length of DNA could be made that contained neither the point mutation, nor the deletion, indicating that the mutations in these two strains cannot be in the same region.Note that not all crossovers between the point mutant and del-2 will yield (+) recombinants; in this case only those crossover events that occur between the point mutant and the 5' end of the deletion would inherit the wild-type sequence.".
- Deletion_mapping wikiPageID "28785745".
- Deletion_mapping wikiPageLength "3040".
- Deletion_mapping wikiPageOutDegree "10".
- Deletion_mapping wikiPageRevisionID "643813472".
- Deletion_mapping wikiPageWikiLink Category:Genetic_engineering.
- Deletion_mapping wikiPageWikiLink DNA.
- Deletion_mapping wikiPageWikiLink Deletion_(genetics).
- Deletion_mapping wikiPageWikiLink Gene.
- Deletion_mapping wikiPageWikiLink Genetic_engineering.
- Deletion_mapping wikiPageWikiLink Genetic_recombination.
- Deletion_mapping wikiPageWikiLink Genetics.
- Deletion_mapping wikiPageWikiLink Mutation.
- Deletion_mapping wikiPageWikiLink Point_mutation.
- Deletion_mapping wikiPageWikiLink Wild-type.
- Deletion_mapping wikiPageWikiLink Wild_type.
- Deletion_mapping wikiPageWikiLinkText "Deletion map".
- Deletion_mapping wikiPageWikiLinkText "Deletion mapping".
- Deletion_mapping wikiPageWikiLinkText "deletion mapping".
- Deletion_mapping hasPhotoCollection Deletion_mapping.
- Deletion_mapping subject Category:Genetic_engineering.
- Deletion_mapping hypernym Technique.
- Deletion_mapping type Software.
- Deletion_mapping type Discipline.
- Deletion_mapping comment "In genetics and especially genetic engineering, deletion mapping is a technique used to ascertain the location of mutation sites within a gene. The principle of deletion mapping involves crossing a strain which has a point mutation in a gene, with multiple strains who each carry a deletion in a different region of the same gene.".
- Deletion_mapping label "Deletion mapping".
- Deletion_mapping sameAs m.0ddf4h_.
- Deletion_mapping sameAs Q5253648.
- Deletion_mapping sameAs Q5253648.
- Deletion_mapping wasDerivedFrom Deletion_mapping?oldid=643813472.
- Deletion_mapping isPrimaryTopicOf Deletion_mapping.