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- qualification abstract "Cycle of quantification/qualification (Cq) is a parameter used in real-time polymerase chain reaction techniques, indicating the cycle number where a PCR amplification curve meets a predefined mathematical criterion. A Cq may be used for quantification of the target sequence or to determine whether the target sequence is present or not.Two criteria to determine the Cq are used by different thermocyclers:Cycle of Threshold (CT) is the number of cycles required for the fluorescent signal to cross a given value threshold. Usually, the threshold is set above the baseline, about 10 times the standard deviation of the noise of the baseline, to avoid random effects on the CT. However, the threshold shouln't be set much higher than that to avoid reduced reproducibility due to uncontrolled factors. Crossing point (Cp) and Take off point (TOP) are the cycle value of the maximum second derivative of the amplification curve.".
- qualification wikiPageID "47887859".
- qualification wikiPageLength "1690".
- qualification wikiPageOutDegree "6".
- qualification wikiPageRevisionID "683610818".
- qualification wikiPageWikiLink Category:Laboratory_techniques.
- qualification wikiPageWikiLink Category:Molecular_biology.
- qualification wikiPageWikiLink Category:Polymerase_chain_reaction.
- qualification wikiPageWikiLink Category:Real-time_technology.
- qualification wikiPageWikiLink Real-time_polymerase_chain_reaction.
- qualification wikiPageWikiLink Standard_deviation.
- qualification hasPhotoCollection qualification.
- qualification wikiPageUsesTemplate Template:Reflist.
- qualification subject Category:Laboratory_techniques.
- qualification subject Category:Molecular_biology.
- qualification subject Category:Polymerase_chain_reaction.
- qualification subject Category:Real-time_technology.
- qualification hypernym Parameter.
- qualification type Software.
- qualification comment "Cycle of quantification/qualification (Cq) is a parameter used in real-time polymerase chain reaction techniques, indicating the cycle number where a PCR amplification curve meets a predefined mathematical criterion.".
- qualification label "Cycle of quantification/qualification".
- qualification wasDerivedFrom qualification?oldid=683610818.
- qualification isPrimaryTopicOf qualification.