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- Chevron_plot abstract "A chevron plot is a way of representing protein folding kinetic data in the presence of varying concentrations of denaturant that disrupts the protein's native tertiary structure. The plot is known as "chevron" plot because of the canonical v, or chevron shape observed when the logarithm of the observed relaxation rate is plotted as a function of the denaturant concentration. In a two-state system, folding and unfolding rates dominate the observed relaxation rates below and above the denaturation midpoint (Cm). This gives rise to the terminology of folding and unfolding arms for the limbs of the chevron. A priori information on the Cm of a protein can be obtained from equilibrium experiments. In fitting to a two-state model, the logarithm of the folding and unfolding rates is assumed to depend linearly on the denaturant concentration, thus resulting in the slopes mf and mu, called the folding and unfolding m-values, respectively (also called the kinetic m-values). The sum of the two rates is the observed relaxation rate. An agreement between equilibrium m-value and the absolute sum of the kinetic m-values is typically seen as a signature for two-state behavior. Most of the reported denaturation experiments have been carried out at 298 K with either urea or guanidinium chloride (GuHCl) as denaturants.".
- Chevron_plot thumbnail Chevron_plot.JPG?width=300.
- Chevron_plot wikiPageID "6736528".
- Chevron_plot wikiPageLength "7524".
- Chevron_plot wikiPageOutDegree "32".
- Chevron_plot wikiPageRevisionID "673611861".
- Chevron_plot wikiPageWikiLink 298_K.
- Chevron_plot wikiPageWikiLink Biomolecular_structure.
- Chevron_plot wikiPageWikiLink Category:Protein_methods.
- Chevron_plot wikiPageWikiLink Category:Protein_structure.
- Chevron_plot wikiPageWikiLink Chemical_kinetics.
- Chevron_plot wikiPageWikiLink Chevron_(insignia).
- Chevron_plot wikiPageWikiLink Circular_dichroism.
- Chevron_plot wikiPageWikiLink Dead_time.
- Chevron_plot wikiPageWikiLink Debye–Hückel_equation.
- Chevron_plot wikiPageWikiLink Denaturation_(biochemistry).
- Chevron_plot wikiPageWikiLink Denaturation_midpoint.
- Chevron_plot wikiPageWikiLink Downhill_folding.
- Chevron_plot wikiPageWikiLink Equilibrium_unfolding.
- Chevron_plot wikiPageWikiLink File:Chevron_plot.JPG.
- Chevron_plot wikiPageWikiLink Fluorescence.
- Chevron_plot wikiPageWikiLink Guanidinium_chloride.
- Chevron_plot wikiPageWikiLink Hammond_effect.
- Chevron_plot wikiPageWikiLink Hammonds_postulate.
- Chevron_plot wikiPageWikiLink Logarithm.
- Chevron_plot wikiPageWikiLink Phi_value_analysis.
- Chevron_plot wikiPageWikiLink Pressure_jump.
- Chevron_plot wikiPageWikiLink Protein_folding.
- Chevron_plot wikiPageWikiLink Relaxation_rate.
- Chevron_plot wikiPageWikiLink Room_temperature.
- Chevron_plot wikiPageWikiLink Slope.
- Chevron_plot wikiPageWikiLink Spectroscopic.
- Chevron_plot wikiPageWikiLink Spectroscopy.
- Chevron_plot wikiPageWikiLink Stopped_flow.
- Chevron_plot wikiPageWikiLink Temperature_jump.
- Chevron_plot wikiPageWikiLink Tertiary_structure.
- Chevron_plot wikiPageWikiLink Transition_state.
- Chevron_plot wikiPageWikiLink Urea.
- Chevron_plot wikiPageWikiLink Viscosity.
- Chevron_plot wikiPageWikiLinkText "Chevron plot".
- Chevron_plot wikiPageWikiLinkText "chevron plot".
- Chevron_plot hasPhotoCollection Chevron_plot.
- Chevron_plot subject Category:Protein_methods.
- Chevron_plot subject Category:Protein_structure.
- Chevron_plot hypernym Way.
- Chevron_plot type Biophysic.
- Chevron_plot type Method.
- Chevron_plot comment "A chevron plot is a way of representing protein folding kinetic data in the presence of varying concentrations of denaturant that disrupts the protein's native tertiary structure. The plot is known as "chevron" plot because of the canonical v, or chevron shape observed when the logarithm of the observed relaxation rate is plotted as a function of the denaturant concentration.".
- Chevron_plot label "Chevron plot".
- Chevron_plot sameAs m.0glcjk.
- Chevron_plot sameAs Q5094447.
- Chevron_plot sameAs Q5094447.
- Chevron_plot wasDerivedFrom Chevron_plot?oldid=673611861.
- Chevron_plot depiction Chevron_plot.JPG.
- Chevron_plot isPrimaryTopicOf Chevron_plot.