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- Blotto_(biology) abstract "In biology, BLOTTO is a blocking reagent made from nonfat dry milk, phosphate buffered saline, and sodium azide. Its name is an almost-acronym of Bovine Lacto Transfer Technique Optimizer. It constitutes an inexpensive source of nonspecific protein (milk casein) which blocks protein binding sites in a variety of experimental paradigms, notably Southern blots, Western blots, and ELISA. Its use was first reported in 1984 by Johnson and Elder's lab at Scripps. Prior to 1984, partially purified proteins such as bovine serum albumin, ovalbumin, or gelatin from various species had been used as blocking reagents but had the disadvantage of being expensive.".
- Blotto_(biology) wikiPageID "3800142".
- Blotto_(biology) wikiPageLength "1135".
- Blotto_(biology) wikiPageOutDegree "7".
- Blotto_(biology) wikiPageRevisionID "602189092".
- Blotto_(biology) wikiPageWikiLink Biology.
- Blotto_(biology) wikiPageWikiLink Bovine_serum_albumin.
- Blotto_(biology) wikiPageWikiLink Category:Immunology.
- Blotto_(biology) wikiPageWikiLink Milk.
- Blotto_(biology) wikiPageWikiLink Ovalbumin.
- Blotto_(biology) wikiPageWikiLink Phosphate-buffered_saline.
- Blotto_(biology) wikiPageWikiLink Phosphate_buffered_saline.
- Blotto_(biology) wikiPageWikiLink Sodium_azide.
- Blotto_(biology) wikiPageWikiLinkText "Blotto (biology)".
- Blotto_(biology) wikiPageWikiLinkText "Blotto".
- Blotto_(biology) hasPhotoCollection Blotto_(biology).
- Blotto_(biology) wikiPageUsesTemplate Template:Molecular-biology-stub.
- Blotto_(biology) wikiPageUsesTemplate Template:Reflist.
- Blotto_(biology) subject Category:Immunology.
- Blotto_(biology) hypernym Reagent.
- Blotto_(biology) type ChemicalCompound.
- Blotto_(biology) comment "In biology, BLOTTO is a blocking reagent made from nonfat dry milk, phosphate buffered saline, and sodium azide. Its name is an almost-acronym of Bovine Lacto Transfer Technique Optimizer. It constitutes an inexpensive source of nonspecific protein (milk casein) which blocks protein binding sites in a variety of experimental paradigms, notably Southern blots, Western blots, and ELISA. Its use was first reported in 1984 by Johnson and Elder's lab at Scripps.".
- Blotto_(biology) label "Blotto (biology)".
- Blotto_(biology) sameAs بلوتو_(علم_الأحياء).
- Blotto_(biology) sameAs m.0b0nn_.
- Blotto_(biology) sameAs Q4928469.
- Blotto_(biology) sameAs Q4928469.
- Blotto_(biology) wasDerivedFrom Blotto_(biology)?oldid=602189092.
- Blotto_(biology) isPrimaryTopicOf Blotto_(biology).