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- Activity-based_proteomics abstract "Activity-based proteomics, or activity-based protein profiling (ABPP) is a functional proteomic technology that uses chemical probes that react with mechanistically related classes of enzymes. The basic unit of ABPP is the probe, which typically consists of two elements: a reactive group (RG, sometimes called a "warhead") and a tag. Additionally, some probes may contain a binding group which enhances selectivity. The reactive group usually contains a specially designed electrophile that becomes covalently-linked to a nucleophilic residue in the active site of an active enzyme. An enzyme that is inhibited or post-translationally modified will not react with an activity-based probe. The tag may be either a reporter such as a fluorophore or an affinity label such as biotin or an alkyne or azide for use with the Huisgen 1,3-dipolar cycloaddition (also known as click chemistry).A major advantage of ABPP is the ability to monitor the availability of the enzyme active site directly, rather than being limited to protein or mRNA abundance. With classes of enzymes such as the serine proteases and metalloproteases that often interact with endogenous inhibitors or that exist as inactive zymogens, this technique offers a valuable advantage over traditional techniques that rely on abundance rather than activity.Finally, in recent years ABPP has been combined with tandem mass spectrometry enabling the identification of hundreds of active enzymes from a single sample. This technique, known as ABPP-MudPIT (multidimensional protein identificationtechnology) is especially useful for profiling inhibitor selectivity as the potency of an inhibitor can be tested against hundreds of targets simultaneously.ABPP were first reported in the 1990s in the study of proteases.".
- Activity-based_proteomics thumbnail Fp_rhodamine.svg?width=300.
- Activity-based_proteomics wikiPageID "10212831".
- Activity-based_proteomics wikiPageLength "4407".
- Activity-based_proteomics wikiPageOutDegree "27".
- Activity-based_proteomics wikiPageRevisionID "619628441".
- Activity-based_proteomics wikiPageWikiLink Active_site.
- Activity-based_proteomics wikiPageWikiLink Alkyne.
- Activity-based_proteomics wikiPageWikiLink Azide.
- Activity-based_proteomics wikiPageWikiLink Azide-alkyne_Huisgen_cycloaddition.
- Activity-based_proteomics wikiPageWikiLink Biotin.
- Activity-based_proteomics wikiPageWikiLink Category:Genomics.
- Activity-based_proteomics wikiPageWikiLink Category:Protein_methods.
- Activity-based_proteomics wikiPageWikiLink Category:Proteomics.
- Activity-based_proteomics wikiPageWikiLink Click_chemistry.
- Activity-based_proteomics wikiPageWikiLink Covalent_bond.
- Activity-based_proteomics wikiPageWikiLink DIFP.
- Activity-based_proteomics wikiPageWikiLink Diisopropyl_fluorophosphate.
- Activity-based_proteomics wikiPageWikiLink Electrophile.
- Activity-based_proteomics wikiPageWikiLink Enzyme.
- Activity-based_proteomics wikiPageWikiLink Enzyme_inhibitor.
- Activity-based_proteomics wikiPageWikiLink Fluorophore.
- Activity-based_proteomics wikiPageWikiLink Huisgen_1,3-dipolar_cycloaddition.
- Activity-based_proteomics wikiPageWikiLink MAFP.
- Activity-based_proteomics wikiPageWikiLink Mass_spectrometry.
- Activity-based_proteomics wikiPageWikiLink Metalloprotease.
- Activity-based_proteomics wikiPageWikiLink Metalloproteinase.
- Activity-based_proteomics wikiPageWikiLink Methoxy_arachidonyl_fluorophosphonate.
- Activity-based_proteomics wikiPageWikiLink Nucleophile.
- Activity-based_proteomics wikiPageWikiLink Post-translational_modification.
- Activity-based_proteomics wikiPageWikiLink Proteomics.
- Activity-based_proteomics wikiPageWikiLink Serine_protease.
- Activity-based_proteomics wikiPageWikiLink Tandem_mass_spectrometry.
- Activity-based_proteomics wikiPageWikiLink Zymogen.
- Activity-based_proteomics wikiPageWikiLink File:Fp_rhodamine.svg.
- Activity-based_proteomics wikiPageWikiLink File:Gel-abpp_eg.png.
- Activity-based_proteomics wikiPageWikiLinkText "Activity-based proteomics".
- Activity-based_proteomics hasPhotoCollection Activity-based_proteomics.
- Activity-based_proteomics wikiPageUsesTemplate Template:Genomics-footer.
- Activity-based_proteomics wikiPageUsesTemplate Template:Reflist.
- Activity-based_proteomics wikiPageUsesTemplate Template:Sections.
- Activity-based_proteomics wikiPageUsesTemplate Template:Technical.
- Activity-based_proteomics subject Category:Genomics.
- Activity-based_proteomics subject Category:Protein_methods.
- Activity-based_proteomics subject Category:Proteomics.
- Activity-based_proteomics hypernym Technology.
- Activity-based_proteomics type Company.
- Activity-based_proteomics type Genomic.
- Activity-based_proteomics type Method.
- Activity-based_proteomics type Omic.
- Activity-based_proteomics type Proteomic.
- Activity-based_proteomics comment "Activity-based proteomics, or activity-based protein profiling (ABPP) is a functional proteomic technology that uses chemical probes that react with mechanistically related classes of enzymes. The basic unit of ABPP is the probe, which typically consists of two elements: a reactive group (RG, sometimes called a "warhead") and a tag. Additionally, some probes may contain a binding group which enhances selectivity.".
- Activity-based_proteomics label "Activity-based proteomics".
- Activity-based_proteomics sameAs m.02q5clf.
- Activity-based_proteomics sameAs Q4677620.
- Activity-based_proteomics sameAs Q4677620.
- Activity-based_proteomics wasDerivedFrom Activity-based_proteomics?oldid=619628441.
- Activity-based_proteomics depiction Fp_rhodamine.svg.
- Activity-based_proteomics isPrimaryTopicOf Activity-based_proteomics.