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- Abscription abstract "During normal transcription, RNA polymerase transcribes a number of short nonproductive oligonucleotides, and this process is called abortive transcription. The trapped RNAPs have been named abscriptases and the synthesis of specific length oligonucleotides called abscription. Artificial promoters, or abortive promoter cassettes (APCs) are used to make a different abscript of specific length and sequence which can be separated and quantified. Abscription efficiently produces trinucleotides at approximately 1,000 to 10,000 abscripts per minute.nGpA-OH + nGTP + APC encoding 5'GpApG + Abscriptase ↔ nGpApG (trinucleotide RNA) + nPPi (inorganic pyrophosphate)A uniform population of trinucleotides are produced in the presence of a dinucleotide initiator (such as GpA) and a ribonucleoside-triphosphate (such as GTP) encoded by the attached APC. In this example, the attached APC carries the DNA sequence CpTpC and would encode the reiterative syntheis of the ribonucleotide GpApG. A single polymerization step will produce a trinucleotide abscript plus inorganic pyrophosphate. Either or both the trinucleotide or the pyrophosphate produced, or the polymerization step itself, can be detected and quantified. Unmodified abscripts are detected and quantified by LC-MS (liquid chromatography-mass spectrometry or rTLC (rapid thin layer chromatography), but the use of modified nucleotides as abscription substrates allows for other types of detection (capillary electrophoresis, fluorescence). Modifications at the 5’ end of the dinucleotide initiator produces fluorescent abscripts. Attachment of biotin at the 5’ end allows abscript capture.Molecular biomarkers such as RNA, protein, and DNA (including DNA methylation, amplifications, deletions, insertions, translocations and mutations) can be detected and quantified by covalently attaching an APC to the target. Different APCs, encoding different abscripts, can be attached to different targets for multiplex detection. The abscripts produced during abscription are quantified and provide a direct measure of the amount of the biomarker to which an Abortive Promoter Cassette (APC) has been attached.".
- Abscription wikiPageID "38567459".
- Abscription wikiPageLength "4311".
- Abscription wikiPageOutDegree "16".
- Abscription wikiPageRevisionID "629431130".
- Abscription wikiPageWikiLink Abortive_initiation.
- Abscription wikiPageWikiLink Abortive_transcription.
- Abscription wikiPageWikiLink Biomarker.
- Abscription wikiPageWikiLink Biomarkers.
- Abscription wikiPageWikiLink Biotin.
- Abscription wikiPageWikiLink Category:Biotechnology.
- Abscription wikiPageWikiLink DNA.
- Abscription wikiPageWikiLink DNA_methylation.
- Abscription wikiPageWikiLink DNA_replication.
- Abscription wikiPageWikiLink Fluorescence.
- Abscription wikiPageWikiLink Fluorescent.
- Abscription wikiPageWikiLink Mutation.
- Abscription wikiPageWikiLink Mutations.
- Abscription wikiPageWikiLink Oligonucleotide.
- Abscription wikiPageWikiLink Oligonucleotides.
- Abscription wikiPageWikiLink Polymerization.
- Abscription wikiPageWikiLink Protein.
- Abscription wikiPageWikiLink Pyrophosphate.
- Abscription wikiPageWikiLink RNA.
- Abscription wikiPageWikiLink Ribonucleoside-triphosphate_reductase.
- Abscription wikiPageWikiLink Rubredoxin—NAD(P)+_reductase.
- Abscription wikiPageWikiLinkText "Abscription".
- Abscription wikiPageWikiLinkText "abortive transcription".
- Abscription wikiPageWikiLinkText "abscription".
- Abscription hasPhotoCollection Abscription.
- Abscription wikiPageUsesTemplate Template:Confusing.
- Abscription wikiPageUsesTemplate Template:Morefootnotes.
- Abscription wikiPageUsesTemplate Template:Reflist.
- Abscription wikiPageUsesTemplate Template:Technical.
- Abscription subject Category:Biotechnology.
- Abscription hypernym Transcription.
- Abscription type Article.
- Abscription type Book.
- Abscription type Article.
- Abscription comment "During normal transcription, RNA polymerase transcribes a number of short nonproductive oligonucleotides, and this process is called abortive transcription. The trapped RNAPs have been named abscriptases and the synthesis of specific length oligonucleotides called abscription. Artificial promoters, or abortive promoter cassettes (APCs) are used to make a different abscript of specific length and sequence which can be separated and quantified.".
- Abscription label "Abscription".
- Abscription sameAs عملية_النسخ.
- Abscription sameAs m.0r4k51m.
- Abscription sameAs Q13217984.
- Abscription sameAs Q13217984.
- Abscription wasDerivedFrom Abscription?oldid=629431130.
- Abscription isPrimaryTopicOf Abscription.