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- Protomap_(proteomics) abstract "PROTOMAP is a recently developed proteomic technology for identifying changes to proteins that manifest in altered migration by one-dimensional SDS-PAGE. It is similar, conceptually, to two-dimensional gel electrophoresis and difference gel electrophoresis in that it enables global identification of proteins that undergo altered electrophoretic migration resulting from, for example, proteolysis or post-translational modification. However, it is unique in that all proteins are sequenced using mass spectrometry which provides information on the sequence coverage detected in each isoform of each protein thereby facilitating interpretation of proteolytic events. PROTOMAP is performed by resolving control and experimental samples in separate lanes of a 1D SDS-PAGE gel. Each lane is cut into evenly spaced bands (usually 15-30 bands) and proteins in these bands are sequenced using shotgun proteomics. Sequence information from all of these bands are bioinformatically integrated into a visual format called a peptograph which plots gel-migration in the vertical dimension (high- to low-molecular weight, top to bottom) and sequence coverage in the horizontal dimension (N- to C-terminus, left to right). A peptograph is generated for each protein the sample (thousands of peptographs are generated from a single experiment) and this data format enables rapid identification of proteins undergoing proteolytic cleavage by making evident changes in gel-migration that are accompanied by altered topography.PROTOMAP stands for PRotein TOpography and Migration Analysis Platform and was invented and developed by Ben Cravatt and colleagues at The Scripps Research Institute.".
- Protomap_(proteomics) wikiPageExternalLink protomap.
- Protomap_(proteomics) wikiPageExternalLink protomap.
- Protomap_(proteomics) wikiPageID "22092644".
- Protomap_(proteomics) wikiPageLength "3515".
- Protomap_(proteomics) wikiPageOutDegree "29".
- Protomap_(proteomics) wikiPageRevisionID "632342426".
- Protomap_(proteomics) wikiPageWikiLink Benjamin_Cravatt_III.
- Protomap_(proteomics) wikiPageWikiLink Bioinformatics.
- Protomap_(proteomics) wikiPageWikiLink Category:Bioinformatics.
- Protomap_(proteomics) wikiPageWikiLink Category:Bioinformatics_software.
- Protomap_(proteomics) wikiPageWikiLink Category:Electrophoresis.
- Protomap_(proteomics) wikiPageWikiLink Category:Laboratory_techniques.
- Protomap_(proteomics) wikiPageWikiLink Category:Mass_spectrometry_software.
- Protomap_(proteomics) wikiPageWikiLink Category:Proteins.
- Protomap_(proteomics) wikiPageWikiLink Category:Proteomics.
- Protomap_(proteomics) wikiPageWikiLink Category:The_Scripps_Research_Institute.
- Protomap_(proteomics) wikiPageWikiLink Difference_gel_electrophoresis.
- Protomap_(proteomics) wikiPageWikiLink Electrophoresis.
- Protomap_(proteomics) wikiPageWikiLink Mass_spectrometry.
- Protomap_(proteomics) wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Protomap_(proteomics) wikiPageWikiLink Post-translational_modification.
- Protomap_(proteomics) wikiPageWikiLink Protease.
- Protomap_(proteomics) wikiPageWikiLink Proteolysis.
- Protomap_(proteomics) wikiPageWikiLink Proteomics.
- Protomap_(proteomics) wikiPageWikiLink Scripps_Research_Institute.
- Protomap_(proteomics) wikiPageWikiLink Shotgun_proteomics.
- Protomap_(proteomics) wikiPageWikiLink Two-dimensional_gel_electrophoresis.
- Protomap_(proteomics) wikiPageWikiLinkText "PROTOMAP".
- Protomap_(proteomics) wikiPageWikiLinkText "Protomap (proteomics)".
- Protomap_(proteomics) wikiPageUsesTemplate Template:About.
- Protomap_(proteomics) wikiPageUsesTemplate Template:Reflist.
- Protomap_(proteomics) subject Category:Bioinformatics.
- Protomap_(proteomics) subject Category:Bioinformatics_software.
- Protomap_(proteomics) subject Category:Electrophoresis.
- Protomap_(proteomics) subject Category:Laboratory_techniques.
- Protomap_(proteomics) subject Category:Mass_spectrometry_software.
- Protomap_(proteomics) subject Category:Proteins.
- Protomap_(proteomics) subject Category:Proteomics.
- Protomap_(proteomics) subject Category:The_Scripps_Research_Institute.
- Protomap_(proteomics) type Biomolecule.
- Protomap_(proteomics) type Biomolecule.
- Protomap_(proteomics) type Biostatistic.
- Protomap_(proteomics) type Laboratory.
- Protomap_(proteomics) type Method.
- Protomap_(proteomics) type Nutrient.
- Protomap_(proteomics) type Omic.
- Protomap_(proteomics) type Process.
- Protomap_(proteomics) type Proteomic.
- Protomap_(proteomics) type Technique.
- Protomap_(proteomics) comment "PROTOMAP is a recently developed proteomic technology for identifying changes to proteins that manifest in altered migration by one-dimensional SDS-PAGE. It is similar, conceptually, to two-dimensional gel electrophoresis and difference gel electrophoresis in that it enables global identification of proteins that undergo altered electrophoretic migration resulting from, for example, proteolysis or post-translational modification.".
- Protomap_(proteomics) label "Protomap (proteomics)".
- Protomap_(proteomics) sameAs Q7252036.
- Protomap_(proteomics) sameAs m.05p77st.
- Protomap_(proteomics) sameAs Q7252036.
- Protomap_(proteomics) wasDerivedFrom Protomap_(proteomics)?oldid=632342426.
- Protomap_(proteomics) isPrimaryTopicOf Protomap_(proteomics).